Identification, characterization and expression of sex-related genes in testes of the giant tiger shrimp Penaeus monodon

Leelatanawit, Rungnapa; Sittikankeaw, Kanchana; Yocawibun, Patchari; Klinbunga, Sirawut; Roytrakul, Sittiruk; Aoki, Takashi; Hirono, Ikuo; Menasveta, Piamsak

doi:10.1016/j.cbpa.2008.09.004

Cited by 90 publications

(54 citation statements)

References 31 publications

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“…SUMOylation is also involved in adult Drosophila gonad development, where genes involved in SUMOylation were expressed in the germline during oogenesis and spermatogenesis, which suggested that SUMOylation is highly active in the germline (Hashiyama et al, 2009). In crustaceans, SUMO cDNAs, have been identified in the shrimp, Litopeanaeus vannamei (Laoong-U-Thai et al, 2009) and testis of the giant tiger shrimp, Penaeus monodon (Leelatanawit et al, 2009); however, to our knowledge, the function of SUMO-1 and Ubc9 in regulation of E. sinensis testis and ovary development has not been studied.…”

Section: Introductionmentioning

confidence: 97%

Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis

Wang

Chen

et al. 2012

Gene

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Section: Introductionmentioning

confidence: 97%

Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis

Wang

Chen

et al. 2012

Gene

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“…The Drosophila species control their sex differentiation process through alternative splicing of pre-mRNA of a series of genes engaged in the sex determination pathway, including sxl , tra , tra-2 , and dsx . In crustacean species, a tra-2 homologue was cloned in P. monodon , but no sex dimorphism on the expression and alternative splicing patterns was discovered [Leelatanawit et al, 2009]. The tra homologue cloned in Daphnia magna also displayed the same mode [Kato et al, 2010].…”

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confidence: 99%

Identification and Characterization of the Sex-Determiner Transformer-2 Homologue in Chinese Shrimp, Fenneropenaeus chinensis

Wen

et al. 2012

Sex Dev

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The transformer-2 gene, encoding a protein (Tra-2) which directs sex-specifically alternative splicing of doublesex (dsx) pre-mRNA in combination with the transformer (Tra) protein, has been proved to play important roles on sex differentiation and sex development in Drosophila melanogaster. In the present study, a tra-2 homologue (FcTra-2) was cloned and characterized in the Chinese shrimp, Fenneropenaeus chinensis. A FcTra-2 genomic DNA sequence with a length of 8,871 bp was obtained and verified to consist of 7 exons and 6 introns. Three alternatively spliced mRNA transcripts, designated as FcTra-2a, FcTra-2b and FcTra-2c, were isolated and characterized. Sequence analysis showed that FcTra-2 included a RNA recognition motif and a linker region, which shared high sequence identities with Tra-2 from other species and 2 arginine/serine rich regions. Further studies were performed on the isoform FcTra-2c, since it exhibited a significantly higher expression level in ovary than in other tissues. In early developmental stages of the shrimp, FcTra-2c was detected to suddenly increase its expression level at the mysis stage. In juvenile stage, FcTra-2c displayed a significantly higher expression level in female Chinese shrimp than in males. These data indicated that FcTra-2 might be involved in female sex determination in Chinese shrimp.

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“…To this end, several studies have applied transcriptomics in the context of sexual development in the commercial Decapods, but the majority have done so through the isolated analyses of certain tissues, namely the gonads [Leelatanawit et al, 2009[Leelatanawit et al, , 2011Jung et al, 2011;Zhang et al, 2011;He et al, 2012;Gao et al, 2014;Jiang et al, 2014;Powell et al, 2015].…”

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confidence: 99%

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster, Sagmariasus verreauxi

Chandler

Aizen²,

Elizur³

et al. 2015

Sex Dev

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The Eastern spiny lobster, Sagmariasus verreauxi, is commercially important in fisheries, with growing aquaculture potential, driving an interest to better understand male sexual differentiation. Amongst the Decapoda, the androgenic gland (AG) and the insulin-like androgenic gland hormone (IAG) have a well-defined function in male sexual differentiation. However, IAG is not a sex determinant and therefore must be considered as part of a broader, integrated pathway. This work uses a transcriptomic, multi-tissue approach to provide an integrated description of male-biased expression as mediated through the AG. Transcriptomic analyses demonstrate that IAG expression is stage- and eyestalk-regulated (low in immature, high in mature and 6-times higher in hypertrophied glands), with IAG being the predominant AG-specific factor. The low expression of this key factor in immature males suggests the involvement of other tissues in male sexual differentiation. Across tissues, the gonad (87.8%) and antennal gland (73.5%) show the highest male-biased differential expression of transcripts and also express 4 sex-determination regulators, known as Dmrts, with broader expression of Sv-Sxl and Sv-TRA-2. In order to better understand male sexual differentiation, tissues other than the AG must also be considered. This research highlights the gonad and antennal gland as showing significant male-biased expression patterns, including the Sv-Dmrts.

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Identification, characterization and expression of sex-related genes in testes of the giant tiger shrimp Penaeus monodon

Cited by 90 publications

References 31 publications

Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis

Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9 in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis

Identification and Characterization of the Sex-Determiner <b><i>Transformer-2</i></b> Homologue in Chinese Shrimp, <b><i>Fenneropenaeus chinensis</i></b>

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

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