Identification, characterization, and function of GRP94 and HSP90β in cold stress response in cold water fish Phoxinus lagowskii

Wang, Sihan; Jiang, Yanzhang; Zhou, Haishui; Sun, Menghong; Ning, Zhaoyang; Mu, Weijie

doi:10.1016/j.aqrep.2022.101443

Cited by 3 publications

(1 citation statement)

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“…In this study, we used a cold acclimation period of 15 days to detect transcriptomic and metabolomic changes induced by a cold environment. The same experimental period or a similar duration, such as two weeks, is often selected [ 102 ] because it has been reported that a rapid establishment of the new steady state from previous homeostasis in critical physiological and biochemical processes requires approximately two weeks in fish after low temperature stress [ 103 – 105 ]. The expression patterns of genes involved in stress response, fatty acid metabolism, glycometabolism, and antioxidant activity by qRT‒PCR in this study indicated that an establishment of new homeostasis at the transcriptional level occurred in the ten days after the cold challenge.…”

Section: Discussionmentioning

confidence: 99%

Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure

Liu,

Tian,

et al. 2023

BMC Genomics

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Teleost fish have evolved various adaptations that allow them to tolerate cold water conditions. However, the underlying mechanism of this adaptation is poorly understood in Tibetan Plateau fish. RNA-seq combined with liquid chromatography‒mass spectrometry (LC‒MS/MS) metabolomics was used to investigate the physiological responses of a Tibetan Plateau-specific teleost, Gymnocypris przewalskii, under cold conditions. The 8-month G. przewalskii juvenile fish were exposed to cold (4 ℃, cold acclimation, CA) and warm (17 ℃, normal temperature, NT) temperature water for 15 days. Then, the transcript profiles of eight tissues, including the brain, gill, heart, intestine, hepatopancreas, kidney, muscle, and skin, were evaluated by transcriptome sequencing. The metabolites of the intestine, hepatopancreas, and muscle were identified by LC‒MS/MS. A total of 5,745 differentially expressed genes (DEGs) were obtained in the CA group. The key DEGs were annotated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. The DEGs from the eight tissues were significantly enriched in spliceosome pathways, indicating that activated alternative splicing is a critical biological process that occurs in the tissues to help fish cope with cold stress. Additionally, 82, 97, and 66 differentially expressed metabolites were identified in the intestine, hepatopancreas, and muscle, respectively. Glutathione metabolism was the only overlapping significant pathway between the transcriptome and metabolome analyses in these three tissues, indicating that an activated antioxidative process was triggered during cold stress. In combination with the multitissue transcriptome and metabolome, we established a physiology-gene‒metabolite interaction network related to energy metabolism during cold stress and found that gluconeogenesis and long-chain fatty acid metabolism played critical roles in glucose homeostasis and energy supply.

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Section: Discussionmentioning

confidence: 99%

Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure

Liu,

Tian,

et al. 2023

BMC Genomics

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Exploring Endoplasmic Reticulum Stress Pathways in Apostichopus japonicus Under Heat Stress Based on Transcriptome Analysis

Yang,

Lu,

et al. 2023

Preprint

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Background High summer temperatures caused by changes in the global environment have significant impacts on marine organisms. The sea cucumber Apostichopus japonicus is a representative marine benthic organism and an economically important shallow-sea aquaculture species in China, and thus it is necessary to explore its response to environmental heat stress. Results Endoplasmic reticulum stress is an important regulatory strategy for organisms to respond to changes in the environment, and thus we analyzed the histology, ultrastructure, and transcriptome of the digestive tract of A. japonicus at three temperatures: normal (18°C), summer dormant (25°C), and lethal (32°C). Histological and ultrastructural results showed that heat stress caused morphological changes in the digestive tract of A. japonicus and inflicted alterations and damage to the endoplasmic reticulum of the enterocytes. Totals of 603 and 4615 differentially expressed genes were identified by transcriptome sequencing in the T25-vs-T18 and the T32-vs-T25 group comparisons, respectively. The GO results showed that DEGs in both group comparisons were significantly enriched in protein folding, chaperonin-mediated protein folding, and other protein-related GO terms. Also, KEGG enrichment analysis showed that both groups activated the pathway of protein processing in the endoplasmic reticulum and induced the endoplasmic reticulum stress response. The endoplasmic reticulum molecular chaperones, including BiP, GRP94, and Hsp70, were all up-regulated in expression. At 32°C, in addition to the above endoplasmic reticulum molecular chaperones, the endoplasmic reticulum molecular receptors PERK, TRAF2, and XBP, which are downstream of endoplasmic reticulum stress in the unfolded protein response, were also up-regulated. Conclusions This study has characterized the internal molecular regulatory process of A. japonicus under heat stress from the perspective of endoplasmic reticulum stress and provides clues regarding the mechanisms behind homeostatic regulation in invertebrates under stress from the environment.

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Characterization of HSP70 and HSP90 Gene Family in Takifugu fasciatus and Their Expression Profiles on Biotic and Abiotic Stresses Response

Zhang,

Qian,

et al. 2024

Genes

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Background: Heat shock proteins (HSPs) play crucial roles in response to temperature changes and biotic stresses. However, the HSP gene family in the pufferfish (Takifugu fasciatus) herring has not been comprehensively investigated. Methods and Results: This study presents a systematic analysis of the HSP70 and HSP90 gene families in T. fasciatus, focusing on gene characterization, conserved structural domains, molecular evolutionary history, and expression patterns of the HSP gene family under stress conditions. The findings reveal that 16 HSP genes are evolutionarily conserved, while hspa4 and hsp90aa appear specific to teleost fish. HSP genes exhibit widespread expression across 11 examined tissues, with most demonstrating high expression levels in the heart, brain, and liver. Furthermore, T. fasciatus was subjected to cryogenic and biotic stresses, revealing distinct expression patterns of HSPs under various stress conditions. The response of HSPs to cold stress and Aeromonas hydrophila infection was sustained. In contrast, gene expression of HSPs significantly changed only in the pre-infection period following Ichthyophthirius multifiliis infection, gradually returning to normal levels in the later stages. Conclusions: These experimental results provide a foundation for further in-depth investigations into the characteristics and functions of HSPs in T. fasciatus.

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Identification, characterization, and function of GRP94 and HSP90β in cold stress response in cold water fish Phoxinus lagowskii

Cited by 3 publications

References 56 publications

Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure

Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure

Exploring Endoplasmic Reticulum Stress Pathways in Apostichopus japonicus Under Heat Stress Based on Transcriptome Analysis

Characterization of HSP70 and HSP90 Gene Family in Takifugu fasciatus and Their Expression Profiles on Biotic and Abiotic Stresses Response

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