Identification, characterization and selection of autochthonous lactic acid bacteria as probiotic for feedlot cattle

Maldonado, Natalia C.; Ficoseco, Cecilia Aristimuño; Mansilla, Flavia I.; Melián, Constanza; Hébert, Elvira María; Vignolo, Graciela; Nader‐Macías, María Elena Fátima

doi:10.1016/j.livsci.2018.04.003

Cited by 36 publications

(35 citation statements)

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“…However, safety concerns have been raised by the use of LAB strains carrying AR genes themselves, as they can potentially transfer them to other commensal and/or pathogenic bacteria through horizontal gene mechanisms ( Imperial and Ibana, 2016 ). Thus, to use as probiotics, safety traits of forty LAB strains previously isolated and identified from feedlot cattle environment ( Maldonado et al, 2018 ), were investigated. The MIC of eight antimicrobial agents for 40 LAB strains involving Lactobacillus (27), Pediococcus (2), Enterococcus (11) strains, was determined.…”

Section: Resultsmentioning

confidence: 99%

“…Nonetheless, as reported by Danielsen et al (2007) , pediococci are intrinsically resistant to TET in addition to VAN. On the other hand, enterococci as commensal inhabitants of the GIT of warm-blooded animals were dominant in feedlot environment ( Maldonado et al, 2018 ). Since this genus emerged as important human and veterinary pathogen/opportunist, the incidence of antimicrobial resistance and virulence determinants were also investigated.…”

Section: Resultsmentioning

confidence: 99%

“…The unexpected high percentage of feedlot enterococci resistant to CLI (82%), ERY (100%) and TET (73%) are concordant with that reported for dairy/bison cattle and pet feces ( Anderson et al, 2008 ; Jackson et al, 2010 ; Iseppi et al, 2015 ; Beukers et al, 2015 ). Among the recovered enterococci from feedlot steers feces, E. hirae was revealed to predominate ( Maldonado et al, 2018 ) and was also described among the highest antibiotic resistant enterococci species ( Beukers et al, 2015 ). In addition, multi-resistance to at least three antimicrobial agents were found for 30% of feedlot strains in which CLI was mostly involved for enterococci strains ( Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Forty LAB previously isolated from feedlot cattle environment and selected for their beneficial characteristics ( Maldonado et al, 2018 ) were used, including Lactobacillus (L.) acidophilus (3), L. amylovorus (4), L. casei (1), L. fermentum (1), L. mucosae (14), L. plantarum (3), L. rhamnosus (1), Pediococcus (P.) acidilactici (2), Enterococcus (E.) durans (3), E. faecium (2) and E. hirae (6). Inoculum of strains were prepared by transferring glycerol stock culture to MRS broth (Merck, Darmstadt, Germany) and sub-cultured twice in the same media at 37°C for 16 h.…”

Section: Methodsmentioning

confidence: 99%

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Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design

et al. 2018

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In order to eliminate the widespread use of antibiotics in livestock production, the research for alternatives has increased lately. This study examined the safety of 40 lactic acid bacteria (LAB) isolated from bovine feedlot environment and previously selected as potential probiotics. A high sensitivity prevalence to ampicillin (AMP, 100%), gentamicin (GEN, 96.3%), kanamycin (KAN, 96.3%), clindamycin (CLI, 85.2%), chloramphenicol (CHL, 92.6%) and streptomycin (STR, 88.9%) while moderate and high resistance against erythromycin (ERY, 48%) and tetracycline (TET, 79%) respectively, were determined. Feedlot enterococci and pediococci displayed high resistance to CLI, ERY, GEN and TET (73, 100, 54.5, and 73%, respectively). Among fifteen resistance genes investigated, seven were identified in lactobacilli; their presence not always was correlated with phenotypic resistance. STR resistance genes, aadA and ant(6) were observed in 7.4 and 3.7% of isolates, respectively; genes responsible for aminoglycosides resistance, such as bla (7.4%), and aph(3”)-III (3.7%) were also recognized. In addition, resistance cat and tetS genes (3.7 and 7.4%, respectively) were harbored by feedlot lactobacilli strains. The presence of ermB gene in 22.3% of isolates, including two of the six strains phenotypically resistant to ERY, exhibited the highest prevalence among the assessed antibiotics. None of the feedlot lactobacilli harbored virulence factors genes, while positive PCR amplification for ace, agg, fsrA, and atpA genes was found for enterococci. With the objective of producing large cell biomass for probiotic delivery, growth media without peptone but containing glucose and skim milk powder (Mgl and Mlac) were selected as optimal. Lactobacillus acidophilus CRL2074, L. amylovorus CRL2115, L. mucosae CRL2069, and L. rhamnosus CRL2084 were strains selected as free of antibiotic resistance and virulence determinants, able to reach high cell numbers in non-expensive culture media and being compatible among them.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design

et al. 2018

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“…Yet, microorganisms are equally as critical for the normal function of numerous body systems [25][26][27][28]. Studies have continued to demonstrate the mutual, commensal and parasitic potential microorganisms impart on these ruminant systems [29][30][31], and until the turn of the century, little knowledge had been gained regarding the microbial impact on ruminant production. Through the advent of modern nucleotide sequencing technologies, novel microbial methods and tools have emerged that have enabled researchers and producers to investigate biological systems with further resolution, specifically with regard to the components contributing to the variation guiding such production efficiencies.…”

Section: Introductionmentioning

confidence: 99%

Microbiomes in ruminant protein production and food security.

Myer¹,

Clemmons²,

Schneider³

et al. 2019

CABI Reviews

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The global population is rapidly increasing and will surpass 10 billion people within the next 20 years. As diminishing resources continue to impact agriculture, and with the necessity to feed the world by 2050, the agricultural sector must be able to sustainably and efficiently produce high-quality sources of food that are both attainable to the global population and contribute to healthy, balanced nutrition. Ruminants are a unique contributor towards a sustainable and food secure world, as they are available and utilized across all economic and social demographics, and can produce high-quality protein from otherwise inedible plants from land that is typically unsuitable for crop production or cultivation. Thus, developing tools, methodologies, and systems for optimizing the production of protein from ruminants stands to make great impacts on food security. Breeding and genetics have played a role in this development, but cannot be a singular solution. Microbes are present at abundances that equal or exceed host cell counts, are ubiquitous throughout all mammalian systems and are required for regular host-physiological functions. Optimizing these host-microbe-symbioses in ruminants permits the opportunity to augment the utility and efficiency of microbiomes and their functions to produce production-specific phenotypes and outputs. This review, therefore, examines the role of microbiomes in ruminants to efficiently and sustainably produce high-quality protein for human consumption to aid in efforts to achieve global food security.

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