Identification, Charecterization and Quantification of Process Related Impurities in Azimsulfuron Technical

Abstract

This manuscript describes identification, isolation and characterization of azimsulfuron impurities. Six impurities ranging from 0.05 to 0.15 % w/w in azimsulfuron technical were detected by a simple reverse phase HPLC method. These impurities were isolated by prep-HPLC and were characterized using IR, 1 H NMR, 13 C NMR and LC-MS/MS. Based on the results obtained from different spectroscopic experiments, these impurities have been characterized as impurity A (2-amino-4, 6-dimethoxy pyrimidine), impurity B (1-methyl-4-(2-methyl-2H-tetrazol-5-yl)-1H-pyrazole-5sulfonamide) impurity C (phenol), impurity D (N-[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]-1methyl-4-(1-methyl-1H-tetrazol-5-yl)-1H-pyrazole-5-sulfonamide), impurity E (1,3-bis(4,6dimethoxypyrimidin-2-yl)urea) and impurity F (phenyl (4,6-dimethoxypyrimidin-2-yl) carbamate).