Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress

Chen, Shuangshuang; Jiang, Jing; Han, Xiaojiao; Zhang, Yun-Xing; Zhuo, Renying

doi:10.3390/ijms19041216

Cited by 35 publications

(22 citation statements)

References 55 publications

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“…Activities of SOD, APX and CAT in transgenic chrysanthemum overexpressing CmHsfA4, were higher than those of WT lines under salt stress . In addition, Hsf activates the expression of downstream genes (including Hsps) and shows a similar pattern of expression to protect plants from external stress (Snyman and Cronjé, 2008;Xue et al, 2014;Xue et al, 2015;Chen et al, 2018). In the present study, we also observed that transgenic Arabidopsis and NHE S. alfredii expressing SaHsfA4c showed higher CAT, POD, and APX activities than those of the WT after Cd stress, and were able to induce the expression of ROS-scavenging system related genes (POD, CAT and APX) and Hsps.…”

Section: Discussionsupporting

confidence: 77%

“…We previously observed that 18 SaHsfs showed a high expression level among the 22 members of the Hsf gene family identified in hyperaccumulation ecotype (HE) S. alfredii after Cd treatment. Furthermore, SaHsfA4c improves the Cd tolerance and accumulation in transgenic yeast (Chen et al, 2018). The function of SaHsfA4c in the regulation of Cd tolerance, however, remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Chen

et al. 2020

Front. Plant Sci.

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The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup from Sedum alfredii. Designated as SaHsfA4c, the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression of SaHsfA4c in yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcript InSaHsfA4c which contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function of SaHsfA4c under Cd stress was characterized in transgenic Arabidopsis and nonhyperaccumulation ecotype S. alfredii. SaHsfA4c was able to rescue the Cd sensitivity of the Arabidopsis athsfa4c mutant. SaHsfA4c reduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes and Hsps in transgenic lines. The present results suggest that SaHsfA4c increases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression of Hsps.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Chen

et al. 2020

Front. Plant Sci.

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“…Previous studies revealed 78 HSFs in wheat (Zhou et al, 2019), 35 in carrot (Huang et al, 2015), and 25 in tea (Zhang et al, 2020c). Based on the relationship between protein sequence, structure, and evolution, there are three types of members in the HSF family in plants: A, B, and C. All types of HSFs in various plants have great similarity (Zhu et al, 2017b;Chen et al, 2018). HSF in class A is mainly responsible for the regulation of heat shock gene expression; while HSF in class B has DNA-binding activity but has no heat shock-induced transcriptional activation activity, which may be a coregulator or inhibitor of genes in class A, and the functions of HSF in class C are unclear (Hu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the Heat Shock Transcription Factor Gene Family in Brassica juncea: Structure, Evolution, and Expression Profiles

Xie

et al. 2020

DNA and Cell Biology

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Heat shock transcription factor (HSF) is ubiquitous in the whole biological world and plays an important role in regulating growth and development and responses to environment stress. In this study, a total of 60 HSF transcription factors in Brassica juncea genome were identified and analyzed. Phylogenetic analysis showed that HSF genes were divided into three groups namely: A, B, and C, of which group A was further divided into nine subgroups (A1-A9). The analysis of gene structure and conserved motifs showed that some homologous genes are highly conserved. There was strong conservative microcollinearity among Brassica rapa, B. juncea, and Brassica oleracea, which provides a basis for studying the replication of gene families. Moreover, the results revealed that the promoter regions of BjuHSF genes were rich in cis-elements related to growth and development, hormone signal, and stress response. The prediction of protein interaction results showed that HSFs could interact with multiple transcription factors and proteins in the genome, while functional annotation revealed that BjuHSF genes were involved in many biological processes. The expression patterns of BjuHSF genes were analyzed by qPCR, and the results showed that these genes were closely linked to stress response, hormones, and development process. These results are a foundation for further analysis of the regulation mechanism of HSF gene family.

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“…The OrthoMCL algorithm (Li et al 2003) (Finn et al 2011) was conducted to identify and screen for possible SaGELPs containing Lipase_GDSL (Pfam: PF00657) domains in S. alfredii (Chen et al 2018). The GELP homologous sequences in A. thaliana were obtained from TAIR (https://www.arabidopsis.org/index.jsp).…”

Section: Identification Of Sagelps In S Alfredii Hancementioning

confidence: 99%

“…Quantitative Real-Time PCR (qRT-PCR) reactions were carried out using the SYBR ® Green premix Ex Taq TM (TaKaRa) reagent on the thermal circulator of an Applied Biosystems 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) (Chen et al 2018). Sequences of primers used in qRT-PCR are shown in Table S1.…”

Section: Total Rna Isolation and Expression Analysismentioning

confidence: 99%

Peer Review #2 of "Identification and expression analysis of the GDSL esterase/lipase family genes, and the characterization of SaGLIP8 in Sedum alfredii Hance under cadmium stress (v0.1)"

2019

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The herb Sedum alfredii (S. alfredii) Hance is a hyper-accumulator of heavy metals [cadmium (Cd), zinc (Zn) and lead (Pb)]; therefore, it could be a candidate plant for efficient phytoremediation. The GDSL esterase/lipase protein (GELP) family plays important roles in plant defense and growth. Although the GELP family members in a variety of plants have been cloned and analyzed, there are limited studies on the family's responses to heavy metal-stress conditions. Methods: Multiple sequence alignments and phylogenetic analyses were performed according to the criteria described. A WGCNA was used to construct co-expression regulatory networks. The roots of S. alfredii seedlings were treated with 100 µM CdCl 2 for qRT-PCR to analyze expression levels in different tissues. SaGLIP8 was transformed into the Cd sensitive mutant strain yeast Δycf1 to investigate its role in resistance and accumulation to Cd. Results: We analyzed GELP family members from genomic data of S. alfredii. A phylogenetic tree divided the 80 identified family members into three clades. The promoters of the 80 genes contained certain elements related to abiotic stress, such as TC-rich repeats (defense and stress responsiveness), heat shock elements (heat stress) and MYB-binding sites (drought-inducibility). In addition, 66 members had tissue-specific expression patterns and significant responses to Cd stress. In total, 13 hub genes were obtained, based on an existing S. alfredii transcriptome database, that control 459 edge genes, which were classified into five classes of functions in a co-expression subnetwork: cell wall and defense function, lipid and esterase, stress and tolerance, transport and transcription factor activity. Among the hub genes, Sa13F.102 (SaGLIP8), with a high expression level in all tissues, could increase Cd tolerance and accumulation in yeast when overexressed. Conclusion: Based on genomic data of S. alfredii, we conducted phylogenetic analyses, as well as conserved domain, motif and expression profiling of the GELP family under Cd-stress conditions. SaGLIP8 could increase Cd tolerance and accumulation in yeast. These results indicated the roles of GELPs in plant responses to heavy metal exposure and provides a theoretical basis for further studies of the SaGELP family's functions.

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Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress

Cited by 35 publications

References 55 publications

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Genome-Wide Analysis of the Heat Shock Transcription Factor Gene Family in Brassica juncea: Structure, Evolution, and Expression Profiles

Peer Review #2 of "Identification and expression analysis of the GDSL esterase/lipase family genes, and the characterization of SaGLIP8 in Sedum alfredii Hance under cadmium stress (v0.1)"

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