Identification, genomic organization and expression pattern of glutathione transferase in Pardosa pseudoannulata

Liu, Wei; Tian, Jing; Hou, Nannan; Yu, Na; Zhang, Yixi; Liu, Zewen

doi:10.1016/j.cbd.2019.100626

Cited by 7 publications

(3 citation statements)

References 56 publications

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“…Allergenic delta-GSTs have been identified in cockroaches ( 32 , 33 ). Their presence in other arthropods different from insects has not been fully investigated, although there are reports of delta-GSTs in Arachnida, including the Acari subclass ( 34 , 35 ). Dougall et al identified a delta-like GST in Dermatophagoides pteronyssinus , but this sequence also showed similarity with epsilon-GSTs ( 20 ).…”

Section: Biological Aspects Of Gstsmentioning

confidence: 99%

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Allergological Importance of Invertebrate Glutathione Transferases in Tropical Environments

2021

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Glutathione-S transferases (GSTs) are part of a ubiquitous family of dimeric proteins that participate in detoxification reactions. It has been demonstrated that various GSTs induce allergic reactions in humans: those originating from house dust mites (HDM), cockroaches, and helminths being the best characterized. Evaluation of their allergenic activity suggests that they have a clinical impact. GST allergens belong to different classes: mu (Blo t 8, Der p 8, Der f 8, and Tyr p 8), sigma (Bla g 5 and Asc s 13), or delta (Per a 5). Also, IgE-binding molecules belonging to the pi-class have been discovered in helminths, but they are not officially recognized as allergens. In this review, we describe some aspects of the biology of GST, analyze their allergenic activity, and explore the structural aspects and clinical impact of their cross-reactivity.

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Section: Biological Aspects Of Gstsmentioning

confidence: 99%

“…In this case, studies of the gene locus, substrate-specificity experiments, and evidence of cross-reactivity with a specific class may help to better define its classification. Insects tend to have numerous delta-GST paralogous genes ( 34 ). For example, delta class represents 39% of all GST genes in A. gambiae ( 31 ).…”

Section: Biological Aspects Of Gstsmentioning

confidence: 99%

Allergological Importance of Invertebrate Glutathione Transferases in Tropical Environments

2021

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“…GSTs, depending on their protein sequence and structure, are known as different superfamilies: cytosolic (cGSTs), mitochondrial (mGSTs), and microsomal (MGSTs) [6]. In mammals, cytosolic GSTs are further categorized, based on sequence similarity, into seven classes: alpha (a), zeta (Z), theta (T), mu (M), pi (P), sigma (S), and omega (O) [7].…”

Section: Introductionmentioning

confidence: 99%

Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1

Perperopoulou

Poudel

Papageorgiou

et al. 2022

Life

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Glutathione transferases (GSTs; EC. 2.5.1.18) are a large family of multifunctional enzymes that play crucial roles in the metabolism and inactivation of a broad range of xenobiotic compounds. In the present work, we report the kinetic and structural characterization of the isoenzyme GSTM1-1 from Camelus dromedarius (CdGSTM1-1). The CdGSΤM1-1 was expressed in E. coli BL21 (DE3) and was purified by affinity chromatography. Kinetics analysis showed that the enzyme displays a relative narrow substrate specificity and restricted ability to bind xenobiotic compounds. The crystal structures of CdGSΤM1-1 were determined by X-ray crystallography in complex with the substrate (GSH) or the reaction product (S-p-nitrobenzyl-GSH), providing snapshots of the induced-fit catalytic mechanism. The thermodynamic stability of CdGSTM1-1 was investigated using differential scanning fluorimetry (DSF) in the absence and in presence of GSH and S-p-nitrobenzyl-GSH and revealed that the enzyme’s structure is significantly stabilized by its ligands. The results of the present study advance the understanding of camelid GST detoxification mechanisms and their contribution to abiotic stress adaptation in harsh desert conditions.

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Transcriptome analysis of wolf spider Pardosa laura exposed to beta-cypermethrin

Ning

Wang

et al. 2021

Int J Trop Insect Sci

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Identification, genomic organization and expression pattern of glutathione transferase in Pardosa pseudoannulata

Cited by 7 publications

References 56 publications

Allergological Importance of Invertebrate Glutathione Transferases in Tropical Environments

Allergological Importance of Invertebrate Glutathione Transferases in Tropical Environments

Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1

Transcriptome analysis of wolf spider Pardosa laura exposed to beta-cypermethrin

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