Identification, growth, and pigment content of a spontaneous green mutant of Pyropia kinositae (Bangiales, Rhodophyta)

Sano, Fumina; Murata, Koki; Niwa, Kyosuke

doi:10.1007/s10811-020-02085-5

Cited by 15 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The chloroplast RUBISCO spacer region, the rbc L gene, and the nuclear V9 region of 18S rRNA were amplified with primer pairs from Sano et al . (2020), Hanyuda et al . (2004) and Yang et al .…”

Section: Methodsmentioning

confidence: 99%

“…Total DNA was extracted using the ISOPLANT II kit (Nippon Gene, Tokyo, Japan) following the method of Niwa et al (2005). The chloroplast RUBISCO spacer region, the rbcL gene, and the nuclear V9 region of 18S rRNA were amplified with primer pairs from Sano et al (2020), Hanyuda et al (2004) and Yang et al (2020), respectively. For sequence analysis of the rbcL gene, two additional primers, Rh1-2 and LS1-2, were also used, as previously described (Niwa et al 2008).…”

Section: Molecular Analyses and Phylogenetic Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Confirmation of Neoporphyra cf. dentata on Shikinejima, Izu Islands, southcentral Japan, and comparison with co‐occurring Neoporphyra haitanensis

Okamoto

Ikeura

Tamaki

et al. 2023

Phycological Research

Self Cite

View full text Add to dashboard Cite

SUMMARYThe Izu Islands of southcentral Japan are thought to fall within the distribution range of Neoporphyra dentata. However, the gametophytic blades of Bangiales collected from Shikinejima and Hachijojima, Izu Islands, were identified as Neoporphyra haitanensis in our previous study. Thus, it became uncertain whether N. dentata is distributed in the Izu Islands, including Shikinejima. To clarify whether N. dentata grows on Shikinejima, we conducted a further distribution survey of N. dentata on the island. The morphological features of the blade samples collected from an additional sampling site on Shikinejima were more similar to those of N. dentata than to those of N. haitanensis: the blade thickness and the division formula of spermatangia resembled those of the former species rather than the latter species. However, the division formula of zygotosporangia was different from those of either species. The phylogenetic analyses of the rbcL gene indicated that the samples were resolved in a clade including N. dentata collected from Shirahama, Chiba Prefecture, and Enoshima, Kanagawa Prefecture, Honshu, Japan. The p‐distances of the chloroplast rbcL gene and nuclear 18S rRNA also supported identification of the samples as N. dentata. The results demonstrated that N. dentata is also distributed on Shikinejima with co‐occurring N. haitanensis, and that the island materials of the two species are genetically different from other materials of the two species, respectively.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Molecular Analyses and Phylogenetic Analysesmentioning

confidence: 99%

Confirmation of Neoporphyra cf. dentata on Shikinejima, Izu Islands, southcentral Japan, and comparison with co‐occurring Neoporphyra haitanensis

Okamoto

Ikeura

Tamaki

et al. 2023

Phycological Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Common solvents applied and tested among researchers, to retrieve PE from red seaweeds, are phosphate buffer [35,46,50,58,69,70,73,78,79,89,90,93] and sodium phosphate buffer [53,103], featured prominently in Table 1. However, solvents such as potassium phosphate buffer [96], distilled water [45,77,83,91], acetic acid-sodium acetate [81,102], citrate buffer [57,74], diethyl ether [74], and EDTA [84], have also been found to be effective.…”

Section: Phycoerythrinmentioning

confidence: 99%

Red Seaweed Pigments from a Biotechnological Perspective

et al. 2021

View full text Add to dashboard Cite

Algae taxa are notably diverse regarding pigment diversity and composition, red seaweeds (Rhodophyta) being a valuable source of phycobiliproteins (phycoerythrins, phycocyanin, and allophycocyanin), carotenes (carotenoids and xanthophylls), and chlorophyll a. These pigments have a considerable biotechnological potential, which has been translated into several registered patents and commercial applications. However, challenges remain regarding the optimization and subsequent scale-up of extraction and purification methodologies, especially when considering the quality and quantity needs, from an industrial and commercial point of view. This review aims to provide the state-of-the-art information on each of the aforementioned groups of pigments that can be found within Rhodophyta. An outline of the chemical biodiversity within pigment groups, current extraction and purification methodologies and challenges, and an overview of commercially available products and registered patents, will be provided. Thus, the current biotechnological applications of red seaweeds pigments will be highlighted, from a sustainable and economical perspective, as well as their integration in the Blue Economy.

show abstract

“…PCR amplifications of the chloroplast RUBISCO spacer region, the rbcL gene, and the nuclear V9 region of the gene encoding ribosomal small subunit 18S rRNA (18S) were conducted using AmpliTaq Gold™ DNA Polymerase (Applied Biosystems™, California, USA) with a T100™ Thermal Cycler (BioRad Laboratories, Inc., California, USA). For sequence analyses of the RUBISCO spacer region, the rbcL gene, and the V9 region of 18S, PCRs were performed by the same procedures as described in Sano et al (2020), Niwa et al (2008), and Yang et al (2018), respectively. The sequences were determined by a DNA sequencing service (FASMAC, Atsugi, Japan).…”

Section: Molecular Analysesmentioning

confidence: 99%

First record of Neoporphyra haitanensis (T.J.Chang & B.F.Zheng) J.Brodie & L.‐E.Yang (Bangiales, Rhodophyta) from Shikinejima Island and Hachijojima Island of the Izu Islands, southern central Japan

Sano

Ikeura

Takasugi

et al. 2021

Phycological Research

Self Cite

View full text Add to dashboard Cite

We conducted a morphological observation and molecular phylogenetic analyses on bladed Bangiales collected from natural populations on Shikinejima Island and Hachijojima Island of the Izu Islands, southern central Japan. The morphological features of the gametophytic blades such as the blade shape, blade margin and reproductive cell divisions were extremely similar to those parameters of Neoporphyra haitanensis, a species cultivated in southern China, and Neoporphyra dentata. However, the blades collected from the two islands were thicker than the blades of N. dentata; this feature was the same as that of N. haitanensis. The chloroplast RUBISCO spacer sequences of present samples were identical to the sequences of N. haitanensis from China. Moreover, phylogenetic positions of three conchocelis strains isolated from the blades on the two islands were examined based on concatenated sequences of the chloroplast rbcL gene and nuclear 18S rRNA. The results showed that the strains were resolved in a clade including N. haitanensis; however, the strains were separated from N. haitanensis in China. Therefore, the bladed Bangiales from the two islands were identified as N. haitanensis. The present study is the first record of N. haitanensis in natural populations in Japan.

show abstract

Identification, growth, and pigment content of a spontaneous green mutant of Pyropia kinositae (Bangiales, Rhodophyta)

Cited by 15 publications

References 23 publications

Confirmation of Neoporphyra cf. dentata on Shikinejima, Izu Islands, southcentral Japan, and comparison with co‐occurring Neoporphyra haitanensis

Confirmation of Neoporphyra cf. dentata on Shikinejima, Izu Islands, southcentral Japan, and comparison with co‐occurring Neoporphyra haitanensis

Red Seaweed Pigments from a Biotechnological Perspective

First record of Neoporphyra haitanensis (T.J.Chang & B.F.Zheng) J.Brodie & L.‐E.Yang (Bangiales, Rhodophyta) from Shikinejima Island and Hachijojima Island of the Izu Islands, southern central Japan

Contact Info

Product

Resources

About