Identification, Molecular Cloning, Expression and Chromosome Mapping of a Family of Transformation Upregulated hnRNP-K Proteins Derived by Alternative Splicing

Dejgaard, Kurt; Leffers, Henrik; Rasmussen, Hanne H.; Madsen, Peder; Kruse, Torben A.; Gesser, Borbala; Nielsen, Henry; Celis, Julio E.

doi:10.1006/jmbi.1994.1116

Cited by 87 publications

(77 citation statements)

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“…K protein is upregulated in malignant cells (Dejgaard et al, 1994;Mandal et al, 2001;Li et al, 2004), and it regulates the expression of genes involved in mitogenic responses, indicating that it has a direct role in cancerogenesis (Michelotti et al, 1995(Michelotti et al, , 1996Ostrowski et al, 2004). We have shown that following mitogenic stimulation, K protein is recruited to gene loci known to be upregulated in malignant states, including c-myc .…”

mentioning

confidence: 83%

“…As K protein is upregulated in tumour cells (Dejgaard et al, 1994;Mandal et al, 2001;Li et al, 2004) and modulates the expression of genes involved in mitogenic responses (Michelotti et al, 1995(Michelotti et al, , 1996Mandal et al, 2001;Ostrowski et al, 2004), altering its properties and introduction of a new phosphorylation site could make K protein either more or less conducive for the cellular processes that lead to tumorigenic state. If so, identification of this novel tumour-associated K protein isoform may prove to be a useful diagnostic, prognostic and/or therapeutic indicator.…”

Section: P<005mentioning

confidence: 99%

“…As a result of alternative splicing, there are several known isoforms of K protein (Dejgaard et al, 1994;Makeyev and Liebhaber, 2002). To explore the possibility that there are isoforms of K protein enriched in cancer, we sequenced cDNA encoding K protein from colorectal adenocarcinoma and compared it to the cDNAs from the surrounding normal mucosa.…”

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confidence: 99%

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Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa

et al. 2006

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The heterogeneous nuclear ribonucleoprotein K (hnRNP K) protein is an RNA-binding protein involved in many processes that compose gene expression. K protein is upregulated in the malignant processes and has been shown to modulate the expression of genes involved in mitogenic responses and tumorigenesis. To explore the possibility that there are alternative isoforms of K protein expressed in colon cancer, we amplified and sequenced K protein mRNA that was isolated from colorectal cancers as well as from normal tissues surrounding the tumours. Sequencing revealed a single G-to-A base substitution at position 274 that was found in tumours and surrounding mucosa, but not in individuals that had no colorectal tumour. This substitution most likely reflects an RNA editing event because it was not found in the corresponding genomic DNAs. Sequencing of RNA from normal colonic mucosa of patients with prior resection of colorectal cancer revealed only the wild-type K protein transcript, indicating that G274A isoform is tumour related. To our knowledge, this is the first example of an RNA editing event in cancer and its surrounding tissue, a finding that may offer a new diagnostic and treatment marker.

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confidence: 83%

Section: P<005mentioning

confidence: 99%

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Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa

et al. 2006

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“…The heterogeneous nuclear ribonucleoprotein K (hnRNP K) protein is an abundant and ubiquitous factor that interacts with a diverse types of molecules including RNA (Dejgaard et al, 1994), DNA (Ostrowski et al, 1994), factors involved in chromatin remodelling Shnyreva et al, 2000), transcription (Du et al, 1993), RNA splicing (Shnyreva et al, 2000) and translation . K protein also interacts with factors involved in signal transduction, including inducible kinases (Weng et al, 1994;Bustelo et al, 1995;Van Seuningen et al, 1995).…”

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confidence: 99%

Nuclear shift of hnRNP K protein in neoplasms and other states of enhanced cell proliferation

Ostrowski

Bomsztyk

2003

Br J Cancer

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The heterogeneous nuclear ribonucleoprotein K (hnRNP K), is a ubiquitously expressed protein that interacts with signal transducers, proteins that modulate gene expression and selective RNA and DNA motifs. K protein is modified in response to extracellular signals and directly regulates rates of transcription and translation. We used serum-treated hepatocyte culture, liver after partial hepatectomy and hepatic neoplasms as systems to compare expression, subcellular distribution and tyrosine phosphorylation of K protein in quiescent and dividing cells. The results show that expression of K protein mRNA was increased in states of enhanced proliferation. Levels of nuclear K protein were also higher in proliferating compared to resting cells. In contrast, levels of cytoplasmic K protein were the same or lower in dividing compared to quiescent cells. States of enhanced proliferation were also associated with increased levels of K protein tyrosine phosphorylation. Nuclear shift of K protein in dividing cells may reflect involvement of K protein in signalling multiple events that regulate expression of genes in proliferating cells.

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“…KH modules have been found in single or multiple copies in a wide variety of quite diverse proteins, from bacteria to human, indicating an early origin in cellular evolution. RNA-binding properties have been demonstrated for several of these proteins, [7][8][9][10][11] suggesting that KH-containing proteins play major roles in regulating cellular RNA metabolism. The presence of the KH motif as the only sequence similarity among these proteins, which are present in diverse cellular locations, strongly suggests that the module itself functions as an RNA-binding domain.…”

Section: Introductionmentioning

confidence: 99%

Exploring protein interiors: The role of a buried histidine in the KH module fold

Fraternali¹,

Amodeo²,

Musco³

et al. 1999

Proteins

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Identification, Molecular Cloning, Expression and Chromosome Mapping of a Family of Transformation Upregulated hnRNP-K Proteins Derived by Alternative Splicing

Cited by 87 publications

References 0 publications

Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa

Editing of hnRNP K protein mRNA in colorectal adenocarcinoma and surrounding mucosa

Nuclear shift of hnRNP K protein in neoplasms and other states of enhanced cell proliferation

Exploring protein interiors: The role of a buried histidine in the KH module fold

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