Identification of 1′-methylascorbigen in broccoli

Potential role of formaldehyde in the mechanism of action of ascorbigens on the basis of BioArena studies

“…The evidence for 'synergism' between copper and HCHO is easily explainable assuming that Cu(II) ions generate (mobilize) HCHO molecules from microbial cells and bind them, forming the [Cu(CH 2 OH) 4 (H 2 O) 2 ] complex (Tyihák et al, 2006). Potential HCHO pools comprise its labile bound forms, mainly as -CH 2 OH groups .…”

“…Furthermore, 1 O 2 can oxidize water, generating, among others, the very reactive O 3 (Wentworth et al, 2002). Therefore, it is proper to speak about the antibacterial (inhibiting/killing) effect of HCHO and its reaction products (Tyihák et al, 2006(Tyihák et al, , 2008a. These reactive molecules are normal components of biological systems.…”

“…4′-Hydroxybenzylascorbigen is the enzymatic degradation product of sinalbin identified in seeds of Sinapis alba (Buskov et al, 2000a, b). Very recent analytical results have proved the presence of 1′-methylascorbigen (MeAG) in samples prepared from fresh broccoli (c < 100 ng × kg −1 fresh weight; Kátay et al, 2006).…”

“…As co-factors one can use compounds which influence the level of the chemically labile, bound HCHO of the cell culture; e.g. L-arginine (Arg) and glutathione (Glut), known as endogenous HCHO capture compounds (Heck et al, 1990;Trézl et al, 1998); dimedone (Dim), an exogenous formaldehyde reagent (Spencer and Henshall, 1955); and Cu(II), an essential trace element which enhances the antibacterial activity of HCHO (Sondossi et al, 1990) and-according to recent observationscan generate HCHO formation from living cells (Tyihák et al, 2006). Moreover, BioArena gives the possibility of extending the monitoring time of processes for several days (Tyihák et al, 2003b(Tyihák et al, , 2005.…”

Simultaneous preparation and characterization of three high-purity type B fumonisins from maize culture

“…In order to obtain high-purity FBs, the extracts need to be further puried by different preparative approaches. The earliest one is thin-layer chromatography (TLC), 22,23 while tedious performances and complicated solvent systems, as well as poor selectivity and low efficiency, limit its application. Centrifugal partition chromatography (CPC) has been utilized for the purication of FB 1 , FB 2 and FB 3 .…”