Identification of 5’ upstream sequence involved in HSPBP1 gene transcription and its downregulation during HIV-1 infection

Iyer, Kruthika; Mitra, Alapani; Mitra, Debashis

doi:10.1016/j.virusres.2022.199034

Cited by 4 publications

(2 citation statements)

References 58 publications

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“…These effects are accomplished through TAR-like or non-TAR promoter regions that bind transcription regulators such as NF-κB and SP1 [ 14 , 67 , 68 , 69 ]. By binding to SP1, Tat can decrease the recruitment of SP1 to the promoter of the heat shock protein (HSP)70 binding protein (HSPBP)1 gene [ 70 ]. This decreases its expression and attenuates HSPBP1-mediated inhibition of viral replication [ 71 ].…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2

Patarca,

Haseltine

2024

IJMS

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Viruses provide vital insights into gene expression control. Viral transactivators, with other viral and cellular proteins, regulate expression of self, other viruses, and host genes with profound effects on infected cells, underlying inflammation, control of immune responses, and pathogenesis. The multifunctional Tat proteins of lentiviruses (HIV-1, HIV-2, and SIV) transactivate gene expression by recruiting host proteins and binding to transacting responsive regions (TARs) in viral and host RNAs. SARS-CoV-2 nucleocapsid participates in early viral transcription, recruits similar cellular proteins, and shares intracellular, surface, and extracellular distribution with Tat. SARS-CoV-2 nucleocapsid interacting with the replication–transcription complex might, therefore, transactivate viral and cellular RNAs in the transcription and reactivation of self and other viruses, acute and chronic pathogenesis, immune evasion, and viral evolution. Here, we show, by using primary and secondary structural comparisons, that the leaders of SARS-CoV-2 and other coronaviruses contain TAR-like sequences in stem-loops 2 and 3. The coronaviral nucleocapsid C-terminal domains harbor a region of similarity to TAR-binding regions of lentiviral Tat proteins, and coronaviral nonstructural protein 12 has a cysteine-rich metal binding, dimerization domain, as do lentiviral Tat proteins. Although SARS-CoV-1 nucleocapsid transactivated gene expression in a replicon-based study, further experimental evidence for coronaviral transactivation and its possible implications is warranted.

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Section: Introductionmentioning

confidence: 99%

Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2

Patarca,

Haseltine

2024

IJMS

View full text Add to dashboard Cite

show abstract

“…These effects are accomplished through TAR-like or non-TAR promoter regions that bind transcription regulators such as NF-κB and SP1 [14,[67][68][69]. By binding to SP1, Tat can decrease the recruitment of SP1 to the promoter of the heat shock protein (HSP)70 binding protein (HSPBP)1 gene [70]. This decreases its expression and attenuates HSPBP1mediated inhibition of viral replication [71].…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2

Patarca,

Haseltine

2024

Preprint

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Viruses continue to provide vital insights into the field of control of gene expression. Viral transactivators, in concert with other viral and cellular proteins, regulate expression of self, other viruses, and host genes with profound effects on infected cells, underlying inflammation, control of immune responses, and pathogenesis. The multifunctional Tat proteins of lentiviruses (HIV-1, HIV-2, and SIV) transactivate gene expression by recruiting host proteins and binding to trans-acting responsive regions (TARs) in viral and host RNAs. SARS-CoV-2 nucleocapsid participates in early viral transcription, recruits similar cellular proteins, and shares intracellular, surface, and extracellular distribution with Tat. SARS-CoV-2 nucleocapsid interacting with the Replication-Transcription complex might, therefore, transactivate viral and cellular RNAs in transcription and reactivation of self and other viruses, acute and chronic pathogenesis, immune evasion, and viral evolution, constituting a potential pan-coronaviral therapeutic target. Here, we show by using primary and secondary structural comparisons that the leaders of SARS-CoV-2 and other coronaviruses contain TAR-like sequences in stem-loops 2 and 3, the coronaviral nucleocapsid C-terminal domains (N-CTDs) harbor a region of similarity to TAR-binding regions of lentiviral Tat proteins, and the coronaviral nonstructural protein 12 has a cysteine-rich metal binding, dimerization domain similar to that in lentiviral Tat proteins.

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Cellular HSF1 expression is induced during HIV-1 infection by activation of its promoter mediated through the cooperative interaction of HSF1 and viral Nef protein

Mitra,

Dasgupta,

Mitra

2024

Archives of Biochemistry and Biophysics

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Identification of 5’ upstream sequence involved in HSPBP1 gene transcription and its downregulation during HIV-1 infection

Cited by 4 publications

References 58 publications

Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2

Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2

Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2

Cellular HSF1 expression is induced during HIV-1 infection by activation of its promoter mediated through the cooperative interaction of HSF1 and viral Nef protein

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