2002
DOI: 10.1677/joe.0.1740395
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Identification of a cDNA encoding a novel amphibian growth hormone-releasing peptide and localization of its transcript

Abstract: Recently, we identified in the bullfrog brain a novel neuropeptide with a C-terminal Leu-Pro-Leu-Arg-Phe-NH 2 sequence. This amphibian neuropeptide was shown to stimulate growth hormone (GH) release in vitro and in vivo and so was designated frog GH-releasing peptide (fGRP). In this study, we cloned a cDNA encoding fGRP from the bullfrog brain by a combination of 3 and 5 rapid amplification of cDNA ends (RACE). The deduced fGRP precursor consisted of 221 amino acid residues, encoding one fGRP and three putativ… Show more

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Cited by 68 publications
(49 citation statements)
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“…Moreover, a series of mass spectrometric analyses verified the expression of nLPXRFa-1 (SVPNLPQRFa), nLPXRFa-2 (MPHASANLPLRFa), nLPXRFa-3 (SIQPLANLPQRFa) and nLPXRFa-4 (APSAGQFIQTLANLPQRFa) as mature endogenous ligands. From the previous (Tsutsui et al 2000, Fukusumi et al 2001, Satake et al 2001, Koda et al 2002, Sawada et al 2002a,b, Ukena et al 2002, Yoshida et al 2003, Ubuka et al 2008, 2009a and present findings (see Fig. 2 and Table 2), it may be stated that the presence of LPXRFa peptides is conserved in vertebrate brains.…”
supporting
confidence: 65%
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“…Moreover, a series of mass spectrometric analyses verified the expression of nLPXRFa-1 (SVPNLPQRFa), nLPXRFa-2 (MPHASANLPLRFa), nLPXRFa-3 (SIQPLANLPQRFa) and nLPXRFa-4 (APSAGQFIQTLANLPQRFa) as mature endogenous ligands. From the previous (Tsutsui et al 2000, Fukusumi et al 2001, Satake et al 2001, Koda et al 2002, Sawada et al 2002a,b, Ukena et al 2002, Yoshida et al 2003, Ubuka et al 2008, 2009a and present findings (see Fig. 2 and Table 2), it may be stated that the presence of LPXRFa peptides is conserved in vertebrate brains.…”
supporting
confidence: 65%
“…Recently, a novel neuropeptide with a C-terminal LPLRFa sequence was identified in the bullfrog (fGRP; Koda et al 2002) and European green frog brain (R-RFa; Chartrel et al 2002). Molecular cloning of cDNA encoding the precursor of fGRP further exhibited that it encodes fGRP and its related peptides (fGRP-RPs; Sawada et al 2002a). fGRP and fGRP-RPs were invariably equipped with -LPXRF (XZL or Q) at their C-termini (Koda et al 2002, Sawada et al 2002a, Ukena et al 2003b.…”
Section: Introductionmentioning
confidence: 99%
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“…The brains were embedded in OCT compound (Miles Inc., Elkhart, IN, USA) and frozen-sectioned frontally at 10 µm thickness on a cryostat at 20 C. The sections were placed onto 3-aminopropyltriethoxysilane-coated slides. In situ hybridization was carried out according to our previous method (Ukena et al 1999, Sawada et al 2002a using the digoxigenin (DIG)-labeled antisense RNA probe. The DIG-labeled antisense RNA probe was produced with RNA labeling kit (Roche Diagnostics) from a part of the peptide precursor cDNA (complementary to nt 313-525).…”
Section: In Situ Hybridization Of Sparrow Gnih Mrnamentioning
confidence: 99%