Identification of a Conserved, Linear Epitope on VP3 of Enterovirus A Species Recognized by a Broad-Spectrum Monoclonal Antibody

Lie, FU; Zhang, Xiaoyu; Jin, Wei-Ping; Wang, Chen; Qian, Shasha; Wang, Mengjun; Wang, Wenhui; Shao, Meng; Guo, Jing; Wang, Zejun; Chen, Xiaoqi; Shen, Shuo

doi:10.3390/v15041028

Cited by 1 publication

(1 citation statement)

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“…They are responsible for very frequent infections, since they are estimated at nearly 1 billion each year worldwide [ 1 , 2 , 3 ]. The genus Enterovirus (EV), of the family Picornaviridae, consists of small, non-enveloped, single-stranded ribonucleic acid (RNA) viruses (27 to 30 nm particles) of positive polarity [ 4 , 5 , 6 , 7 , 8 ] HEVs, including four species (HEV A, B, C and D) and grouping more than 120 serotypes [ 5 , 9 , 10 , 11 ]. EVs’ viral particles are known to be resistant outside the host.…”

Section: Introductionmentioning

confidence: 99%

Molecular Amplification and Cell Culturing Efficiency for Enteroviruses’ Detection in Cerebrospinal Fluids of Algerian Patients Suffering from Meningitis

Rai,

Ammi,

Anes-Boulahbal

et al. 2024

Viruses

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Enteroviruses (EVs) represent a major cause of viral meningitis, being responsible for nearly 1 billion infections each year worldwide. Several techniques were developed to obtain better diagnostic results of EV infections. Herein, we evaluated the efficiency of EV detection through isolation on both Rhabdomyosarcoma (RD) and Vero cell line cultures, conventional reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR. Thus, 50 cerebrospinal fluid (CSF) samples belonging to patients suspected to have viral meningitis in northern Algeria were collected, anonymously numbered from 1 to 50 and subjected to the above-mentioned techniques for EV detection. Using real-time RT-PCR, 34 CSF samples were revealed to be positive for viral origin of meningitis (68%). Thirteen of them were positive when the conventional RT-PCR was used (26%), and only three samples gave positive results when the cell culture technique was used (6%). Surprisingly, two cell culture-positive CSF samples, namely, 31 and 39, were negative using RT-PCR directly on the original samples. However, they turned to be positive when amplification was carried out on their corresponding cell culture supernatant. The cell-cultured viral isolates were then identified by sequencing their viral genome’s VP1 regions. All of them were revealed to belong to the echovirus 27 strain. This investigation demonstrates that RT-PCR techniques are often more sensitive, accurate and much faster, providing reliable results within a clinically acceptable timeframe. However, viral isolation on cell cultures remains crucial to obtain enough viral load for serological tests or even to avoid the rare, but existing, false negative PCR.

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