Identification of a new self-incompatibility allele (S31) in a Hungarian almond cultivar and its reliable detection

Halász, Júlia; Fodor, Agota; Hegedűs, Attila; Pedryc, A.

doi:10.1016/j.scienta.2008.02.009

Cited by 21 publications

(22 citation statements)

References 16 publications

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“…Furthermore, the information obtained in this way, enabled to update the table of crossincompatibility groups (Ortega et al 2006). However, as expected from their consensus nature, these primers are not be able to discriminate between alleles with a second intron of very similar size, as is the case of S 9 and S 31 almond S-RNase alleles (Halász et al 2008). …”

Section: Introductionmentioning

confidence: 94%

Molecular and phenotypic characterization of the S-locus and determination of flowering time in new ‘Marcona’ and ‘Desmayo Largueta’-type almond (Prunus dulcis) selections

et al. 2010

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Marcona' and 'Desmayo Largueta' are the most widely cultivated almonds in Spain, representing around 27% of the total production and 34% of the entire almond growing surface. The excellent quality of their kernels makes them highly appreciated and demanded worldwide. However, due to their selfincompatibility, they should be grown along with cross-compatible cultivars, whose lower kernel quality often reduces the economic benefits of the plantation. In addition, although they are cross-compatible, are not good candidates to share the orchard since 'Desmayo Largueta' usually flowers earlier than 'Marcona'. Therefore, to optimize orchard yield, genotypes with overlapping flowering times, cross-compatible with these cultivars and of similar fruit and ripening characteristics are desirable. In order to find suitable pollinators of these cultivars, five 'Marcona' and four 'Desmayo Largueta'-type selections from ''Instituto Técnico Agronómico Provincial (ITAP) de Albacete'' (Spain) were characterized for flowering time and for self and cross-incompatibility. The results obtained showed that the nine ITAP selections were self-incompatible, and that three and one were promising candidates as pollinators of 'Marcona' and 'Desmayo Largueta', respectively. The S-haplotypes of two ITAP selections were characterized by cloning and sequencing their pistil S (S-RNase) and pollen S (SFB) genes. The results also showed that S f RNase does not have a mutated histidine in C2 region, and detected differences with other previously published sequences for S 23 RNase and SFB 23 allele. Moreover, the sequence for almond SFB 27 allele is reported here for the first time.

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Section: Introductionmentioning

confidence: 94%

Molecular and phenotypic characterization of the S-locus and determination of flowering time in new ‘Marcona’ and ‘Desmayo Largueta’-type almond (Prunus dulcis) selections

et al. 2010

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“…Aquellas secuencias que presentan el mayor porcentaje de identidad con secuencias reportadas en el GenBank son: CAR007A (97 %) con el alelo S7 de P. dulcis reportado por Halász et al [23], H014A (96 %) con el alelo S6 de P. dulcis reportado por Ortega et al, [19]. A continuación se encuentran IMB011B y CAN009A con un 93 % de identidad compartida con el alelo S3 de P. webbii reportado por Banovic et al, [24].…”

Section: Similitudes Con Intrones De S-rnasas De Otras Especies Del Gunclassified

Identificación de alelos S asociados con autoincompatibilidad en individuos de capulí (<em>Prunus serótina</em> subsp. <em>capulí</em>) mediante la amplificación del Intrón I del gen de la S-RNasa

Gordillo

Tobar

Arahana

et al. 2015

Av. Cienc. Ing. (Quito)

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AVANCES Identificación de alelos S asociados con autoincompatibilidad en individuos de capulí (Prunus serotina subsp. capulí) mediante la amplificación del Intrón I del gen de la S-RNasaIdentification of S alleles associated with self-incompatibility in capuli (Prunus serotina subsp. capulí) samples by amplification of the Intron I of the S-RNasa gene AbstractIn plants, gametophytic self-incompatibility is a genetic mechanism regulated by the S locus, which has evolved to prevent self-fertilization. In fruit crops, information regarding the allelic composition of the S locus is essential for the establishment of productive orchards, as this allelic composition defines compatible combinations between individuals. The identification and cloning of S-RNase genes in Prunus species has allowed the development of molecular techniques for the characterization of S genotypes in wild and less-studied species of the genus. In this study we evaluated 80 individuals of capulí (Prunus serotina subsp. capulí) collected from 8 provinces of the Ecuadorian highlands to determinate the degree of allelic diversity of the S locus in this species. The molecular characterization of S loci was performed using degenerate primers designed from conserved regions of the S-RNase gene of several Prunus species. PCR products were separated on agarose gels, classified based on band size and sequenced. Our results reveal the presence of 11 alleles across sampled individuals. Generally, identified alleles showed a high percentage of identity with S-locus sequences reported for other species of the genus and it can be speculated that these derive from a common ancestor. By contrast, sequences with a lower percentage of identity may have originated independently following the diversification of Prunus species. The results obtained in this study should be complemented with field tests to confirm the phenotypic behavior of the capuli individuals analyzed.

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“…(2007) from Italian cultivars. Later, Halász et al. (2008) identified the S 31 ‐allele in a Hungarian cultivar (its label is modified in this study as S 31H ), while at the same time, Kodad et al.…”

mentioning

confidence: 97%

“…2005). To identify the S 31 ‐allele, allele‐specific primer pair was used according to Halász et al. (2008).…”

mentioning

confidence: 99%

S‐genotyping of Eastern European almond cultivars: identification and characterization of new (S₃₆–S₃₉) self‐incompatibility ribonuclease alleles

Halász¹,

Fodor²,

Pedryc³

et al. 2010

Plant Breeding

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Consensus polymerase chain reaction (PCR) primers amplifying the first and second intron regions of the S-RNase gene and DNA sequencing as well as fruit set analysis after controlled pollinations were used for S-genotyping of Eastern European almond cultivars. Complete S-genotypes have been proposed for 19 accessions, and partial S-genotypes have been proposed for three additional accessions. Based on DNA sequences and fruit set analysis, two novel crossincompatibility groups have been proposed: group XXI (S 11 S 31H ) including ÔSzigetcse´pi 55Õ (3/9) and ÔErianeÕ and group XXII (S 36 S 37 ) for ÔNikitskyiÕ and ÔÓ ria´s kagylo´Õ (4/4). Four new alleles were identified, S 36 (FJ529211), S 37 (FJ529212), S 38 (FJ529213) and S 39 (FJ529214). The combination of consensus PCR primers amplifying the first and second intron regions could reliably discriminate the S-RNase alleles identified in this study from those described previously. Our results supply long-awaited information on almond S-allele diversity from regions between the main cultivation centres and the centre of origin of this species.

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Identification of a new self-incompatibility allele (S31) in a Hungarian almond cultivar and its reliable detection

Cited by 21 publications

References 16 publications

Molecular and phenotypic characterization of the S-locus and determination of flowering time in new ‘Marcona’ and ‘Desmayo Largueta’-type almond (Prunus dulcis) selections

Molecular and phenotypic characterization of the S-locus and determination of flowering time in new ‘Marcona’ and ‘Desmayo Largueta’-type almond (Prunus dulcis) selections

Identificación de alelos S asociados con autoincompatibilidad en individuos de capulí (<em>Prunus serótina</em> subsp. <em>capulí</em>) mediante la amplificación del Intrón I del gen de la S-RNasa

S‐genotyping of Eastern European almond cultivars: identification and characterization of new (S₃₆–S₃₉) self‐incompatibility ribonuclease alleles

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Identification of a new self-incompatibility allele (S31) in a Hungarian almond cultivar and its reliable detection

Cited by 21 publications

References 16 publications

Molecular and phenotypic characterization of the S-locus and determination of flowering time in new ‘Marcona’ and ‘Desmayo Largueta’-type almond (Prunus dulcis) selections

Molecular and phenotypic characterization of the S-locus and determination of flowering time in new ‘Marcona’ and ‘Desmayo Largueta’-type almond (Prunus dulcis) selections

Identificación de alelos S asociados con autoincompatibilidad en individuos de capulí (<em>Prunus serótina</em> subsp. <em>capulí</em>) mediante la amplificación del Intrón I del gen de la S-RNasa

S‐genotyping of Eastern European almond cultivars: identification and characterization of new (S36–S39) self‐incompatibility ribonuclease alleles

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S‐genotyping of Eastern European almond cultivars: identification and characterization of new (S₃₆–S₃₉) self‐incompatibility ribonuclease alleles