Identification of a novel selenium metabolite, Se-methyl-N-acetylselenohexosamine, in rat urine by high-performance liquid chromatography–inductively coupled plasma mass spectrometry and –electrospray ionization tandem mass spectrometry

Ogra, Yasumitsu; Ishiwata, Kazuya; Takayama, Hiromitsu; Aimi, Norio; Suzuki, Kazuo

doi:10.1016/s1570-0232(01)00581-5

Cited by 124 publications

(114 citation statements)

References 35 publications

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“…4,5 Further elucidation of the efficacy or toxicity of selenium in human and plant metabolic processes will rely heavily on speciation, since the effect of selenium is dependent on which chemical form is ingested (primarily elemental and selenite), and subsequently expressed in the form of a number of selenoproteins. 6 Selenoand selenosulfur-amino acids appear to be ubiquitous metabolites, 7,8 with selenosugars among the products of excretion. 9 The identification of selenometabolites has come to rely principally on either HPLC-ICP-MS, ESI-MS-MS, 10 or their combination.…”

Section: Introductionmentioning

confidence: 99%

Influence of Speciation on the Response from Selenium to UV-Photochemical Vapor Generation

et al. 2012

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Section: Introductionmentioning

confidence: 99%

Influence of Speciation on the Response from Selenium to UV-Photochemical Vapor Generation

et al. 2012

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“…Currently, four selenosugars were identified in liver, serum and urine of quail [34], turtle [35], rat [36], porcine [37], and human [38], including methyl-2-acetamido-2-deoxy-1-seleno-β-D-galactopyranoside (selenosugar 1) [39,40], methyl-2-acetamido-2-deoxy-1-seleno-β-D-glucosopyranoside (selenosugar 2) [40], and methyl-2-amino-2-deoxy-1-seleno-β-D-galactopyranoside (selenosugar 3) [40] and selenosugar A [41]. In urine samples, selenosugar 1 is the major metabolite.…”

Section: Speciation Of Sementioning

confidence: 99%

Recent advances in speciation analysis of mercury, arsenic and selenium

2012

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Mercury (Hg), arsenic (As) and selenium (Se) are ubiquitous in the environment and exist in a variety of species, which have great influence on their transport, bioaccumulation and toxicity. This review presents the recent research progress in speciation analysis of Hg, As, and Se, with emphasis on enhanced cold vapor generation as interface for liquid chromatography and atomic spectrometry, speciation of volatile species in gas phase, and isotope dilution technique to improve the precision and accuracy of speciation. Hyphenated techniques to characterize the complexes of Hg and As with phytochelatins and chromatographic separation coupled with multi-collector-inductively coupled plasma mass spectrometry to measure species-specific isotopic ratios, are also briefly discussed. Mercury (Hg) and arsenic (As) are among the most highly toxic elements for humans and ecosystems and can cause serious environmental and human health problems even at low concentrations [1,2]. Selenium (Se) is an "essential toxin" for human health. It has been recognized as an important antioxidant and anti-cancer element. However, Se can also pose negative impact such as defective skin and hair loss [3]. The adsorption/uptake, transport, bioaccumulation, metabolism and toxicity of these elements strongly depend on their chemical species present in environmental and biological samples. In the environment, one species can transform to another through chemical or biological processes. For example, in anaerobic sediments, sulfur-reducing or iron-reducing bacteria can methylate inorganic mercury into highly toxic methylmercury (MeHg) [4,5]. Therefore, speciation analysis of Hg, As and Se is of great importance in order to elucidate the biogeochemcal cycle and toxicology of these elements. Speciation analysis mainly involves chromatographic separation (gas chromatography (GC) or high performance liquid chromatography (HPLC)) hyphenated with element-specific spectrometry or molecular mass spectrometry. This review presents the recent advances in speciation analysis of Hg, As, and Se based on chromatographic separation, with emphasis on enhanced cold vapor generation (CVG) as interface for LC and atomic spectrometry, speciation of volatile Hg, As and Se in gas phase, and isotope dilution technique to improve the precision and accuracy of speciation. Hyphenated techniques to characterize the complexes of Hg and As with phytochelatins and chromatographic separation coupled with multi-collector-inductively coupled plasma mass spectrometry (MC-ICP-MS) to measure species-specific isotopic ratios, are also briefly introduced.

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“…As a complementary technique to LC-ICP-MS, electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI)-mass spectrometry was used for the identification of unknown Se-containing metabolites in biological samples. [10][11][12][13][14][15] Since ESI is a softer technique than ICP, it can provide molecular information. 16 Hence, ICP-MS and ESI/APCI-MS are occasionally called inorganic and organic mass spectrometry, respectively.…”

Section: Introductionmentioning

confidence: 99%

Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium Speciation

2015

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Experimental ReagentsSodium selenate and ammonium acetate were purchased from Wako (Osaka, Japan).L-Selenomethionine (SeMet) and trimethylselenonium iodide (TMSe) were purchased from Sigma (St. Louis, MO) and Tri Chemical Laboratories, Inc. 2015 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. E-mail: ogra@chiba-u.jp Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium SpeciationYasumi ANAN,* Genki NAKAJIMA,* and Yasumitsu OGRA* , ** † We demonstrated the complementary use of inductively coupled plasma-mass spectrometry (ICP-MS) and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) for the analysis of Se-containing compounds, such as selenate, selenomethionine (SeMet), and trimethylselenonium ion (TMSe), found in biological samples. The sensitivity of ESI-Q-TOF-MS for Se-containing compounds was strongly dependent on the chemical species. ICP-MS exhibited higher sensitivity than ESI-Q-TOF-MS, and had no species dependency. On the other hand, ESI-Q-TOF-MS enabled easy and robust identification of Se-containing compounds.

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Identification of a novel selenium metabolite, Se-methyl-N-acetylselenohexosamine, in rat urine by high-performance liquid chromatography–inductively coupled plasma mass spectrometry and –electrospray ionization tandem mass spectrometry

Cited by 124 publications

References 35 publications

Influence of Speciation on the Response from Selenium to UV-Photochemical Vapor Generation

Influence of Speciation on the Response from Selenium to UV-Photochemical Vapor Generation

Recent advances in speciation analysis of mercury, arsenic and selenium

Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium Speciation

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