Identification of a Primitive Brain–Derived Neural Stem Cell Population Based on Aldehyde Dehydrogenase Activity

Corti, Stefania; Locatelli, Federica; Papadimitriou, Dimitra; Donadoni, Chiara; Salani, Sabrina; Bo, Roberto Del; Strazzer, Sandra; Bresolin, Nereo; Comi, Giacomo P.

doi:10.1634/stemcells.2005-0217

Cited by 246 publications

(175 citation statements)

References 27 publications

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“…In the hippocampus, these astrocytes are located in the subgranular zone (SGZ). Putative murine neural stem cells, capable of neurosphere formation and with trilineage potential, have been isolated based on either CD133 expression [162] or high ALDH activity combined with low side scatter [163]. A transcriptional regulator, Hmga2, functions to suppress p16 Ink 4a and p19 Arf in the neural stem cells of young mice, but its down-regulation in older mice is thought to contribute to loss of stem cell renewal and a consequent reduction in stem cell numbers [164].…”

Section: Central Nervous Systemmentioning

confidence: 99%

Attributes of adult stem cells

Alison

Islam

2008

The Journal of Pathology

153

115

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While cultured embryonic stem (ES) cells can be harvested in abundance and appear to be the most versatile of cells for regenerative medicine, adult stem cells also hold promise, but the identity and subsequent isolation of these comparatively rare cells remains problematic in most tissues, perhaps with the notable exception of the bone marrow. The ability to continuously self-renew and produce the differentiated progeny of the tissue of their location are their defining properties. Identifying surface molecules (markers) that would aid in stem cell isolation is a major goal. Considerable overlap exists between different putative organspecific stem cells in their repertoire of gene expression, often related to self-renewal, cell survival and cell adhesion. More robust tests of 'stemness' are now being employed, using lineage-specific genetic marking and tracking to show production of long-lived clones and multipotentiality in vivo. Moreover, the characterization of normal stem cells in specific tissues may provide a dividend for the treatment of cancer. The successful treatment of neoplastic disease may well require the specific targeting of neoplastic stem cells, cells that may well have many of the characteristics of their normal counterparts.

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Section: Central Nervous Systemmentioning

confidence: 99%

Attributes of adult stem cells

Alison

Islam

2008

The Journal of Pathology

153

115

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“…On the experiment day, the cultured cells were withdrawn from the flask, following the standard protocol, to apportion on analysis samples 3 .…”

Section: Aldefluor ®mentioning

confidence: 99%

“…When keratinocyte culture is performed, the cells that adhere to the flask proliferate to form large colonies that at confluence fuse to form a sheet of keratinocytes 3 . Cultured epidermal sheets have been extensively used as autografts to treat burn victims, establishing that stem cells survive in culture 3 .…”

Section: ■ Introductionmentioning

confidence: 99%

“…Cultured epidermal sheets have been extensively used as autografts to treat burn victims, establishing that stem cells survive in culture 3 .…”

Section: ■ Introductionmentioning

confidence: 99%

“…Nowadays, stem cells can be isolated from almost all types of tissues 3 . There are some techniques used to separate them after their isolation and growth.…”

Section: ■ Introductionmentioning

confidence: 99%

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Aldefluor protocol to sort keratinocytes stem cells from skin

et al. 2017

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Purpose: To investigate the use Aldefluor ® and N, N -Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor ® aliquots were prepared and maintained at temperature between 2 to 8°C, or stored at -20°C. Next, the cells were collected following the standard protocol of sample preparation. Results: Best results were obtained with Aldefluor ® 1.5µl and DEAB 15 µl for 1 x 10 6 cells, incubated at 37°C for 15 minutes. Flow cytometer range for keratinocyte stem cells separation was evaluated. There were 14.8% of stem cells separated in one sample of keratinocyte culture used to pattern the protocol. After being defined the ideal concentration, the same test pattern was performed in other keratinocyte samples. We observed a final mean of 10.8%. Conclusion: Aldefluor ® has been shown as a favorable marking of epidermal keratinocyte stem cells for subsequent separation on a flow cytometer, with detection of 10.8% of epidermal keratinocyte stem cells, in this protocol.

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Isolation and Identification of Neural Stem/Progenitor Cells

Deleyrolle

2015

Principles of Stem Cell Biology and Cancer

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Identification of a Primitive Brain–Derived Neural Stem Cell Population Based on Aldehyde Dehydrogenase Activity

Abstract: STEM CELLS 2006; 24:975-985

Cited by 246 publications

References 27 publications

Attributes of adult stem cells

Attributes of adult stem cells

Aldefluor protocol to sort keratinocytes stem cells from skin

Isolation and Identification of Neural Stem/Progenitor Cells

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