Identification of a RAPD marker for palmitic-acid concentration in the seed oil of spring turnip rape (Brassica rapa ssp. oleifera)

Tanhuanpää, Pirjo; Vilkki, Juha; Vilkki, H. J.

doi:10.1007/bf00222976

Cited by 24 publications

(8 citation statements)

References 10 publications

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“…Further analyses showed that four (Valine, Alanine, Leucine, Valine) of the eight amino acids (Threonine, Histidine, Valine, Alanine, Histidine, Histidine, Leucine, Valine) belonged to hydrophobic residues in the third histidine cluster and the neighboring amino acid sequences, which implied that these hydrophobic residues may be located in the interior of the active site on the spatial structure. The top ten amino acids were all found to be leucine in the second histidine cluster, which is consistent with the findings of a previous study (Tanhuanpää et al, 1995). This structure might be one of the active sites of the FAD2 enzyme.…”

Section: Discussionsupporting

confidence: 91%

Sequence variations in the FAD2 gene in seeded pumpkins

Chang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. The fatty acid desaturase-2 (FAD2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. However, little is known about sequence variations in FAD2 in seeded pumpkins. Twenty-seven FAD2 clones from 27 accessions of Cucurbita moschata, Cucurbita maxima, Cucurbita pepo, and Cucurbita ficifolia were obtained (totally 1152 bp; a single gene without introns). More than 90% nucleotide identities were detected among the 27 FAD2 clones. Nucleotide substitution, rather than nucleotide insertion and deletion, led to sequence polymorphism in the 27 FAD2 clones. Furthermore, the 27 FAD2 selected clones all encoded the FAD2 enzyme (delta-12 desaturase) with amino acid sequence identities from 91.7 to 100% for 384 amino acids. The same main-function domain between 47 and 329 amino acids was identified. The four species clustered separately based on differences in the sequences that were identified using the unweighted pair group 17482-17488 (2015) method with arithmetic mean. Geographic origin and species were found to be closely related to sequence variation in FAD2.

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Section: Discussionsupporting

confidence: 91%

Sequence variations in the FAD2 gene in seeded pumpkins

Chang

et al. 2015

Genet. Mol. Res.

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“…3). The linkage group containing the SLG6a locus, LG 9 was compared to the corresponding linkage group developed by Tanhuanpaa et al (1995) using some probes common to the present study. This led to the detection of homology of three loci detected by wg2a11, SLG6 and wg5b9 in the same order (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…containing SLG6a locus was compared to the corresponding linkage group developed by Tanhuanpaa et al (1995). Three marker loci wg2a11, SLG (SLG8) and wg5b9 in their map were homologous to wg2a11a, SLG6a and wg5b9 in the present map, the order being the same.…”

Section: Discussionmentioning

confidence: 99%

“…The SI genes have already been mapped in B. oleracea (Kianian and Quiros 1992a,b, Camargo et al 1997, Cheung et al 1997 and B. napus (Cheung et al 1997, Lombard andDelourme 2001). Loci controlling SI (SLG) has been mapped in B. campestris using F 2 mapping populations by Tanhuanpaa et al (1995) andNozaki et al (1997). Mapping of SI controlling locus/loci in permanent mapping populations such as recombinant inbred (RI) population in B. campestris could facilitate precise genetic monitoring of the gene(s) through markerassisted breeding (MAB).…”

Section: Introductionmentioning

confidence: 97%

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RFLP mapping of loci controlling self-incompatibility in Brassica campestris and their comparative mapping with B. napus and B. oleracea

Panigrahi¹,

Kole²,

Kole³

2011

Biologia plant.

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RFLP analysis of a cDNA probe SLG6, governing self incompatibility (SI) in Brassica oleracea, using a recombinant inbred population of Brassica campestris followed by genetic linkage analysis led to the detection of two marker loci, SLG6a and SLG6b controlling SI. SLG6a was mapped in linkage group (LG) 9 and was flanked by the RFLP markers ec4f10 (6.4 cM) and wg5b9 (4.2 cM). SLG6b positioned in LG 2 and was flanked by the RFLP markers wg2d11 (9.9 cM) and ec4e7 (26.9 cM). These results indicated the scope of marker-aided introgression of these genes into selfcompatible genotypes for production of SI lines suitable for hybridization in B. campestris. Comparative mapping of LG 9 containing SLG6b with corresponding linkage groups of B. oleracea (BO 2) and B. napus (BN 16) led to the detection of small homologous regions with SLG6 locus linked with another RFLP locus. This evidenced for homology of the SLG genes across Brassica species and possibility of using any single cloned SLG gene for development of SI lines in any Brassica species.

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“…These include SITL for erucic acid, seed colour, leaf pubescence, and resistance to white rust caused by the fungus Albugo candida (Teutonico and Osborn, 1994;Kole et al, 1996Kole et al, , 1997aKole et al, , 2002a. Several QTL controlling vernalization requirement, fl owering time, winter survival, freezing tolerance, degree of white rust resistance, and contents of palmitic, oleic and linolenic acid have been detected (Tanhuanpaa et al, 1995a(Tanhuanpaa et al, , b, 1996Osborn et al, 1997;Kole et al, 2001Kole et al, , 2002a. A major QTL controlling vernalization responsive fl owering time has already been Mendelized (Kole et al, 1997b(Kole et al, , 2001.…”

Section: Introductionmentioning

confidence: 99%

Addition of Restriction Fragment Length Polymorphism Markers to the Genetic Linkage Map of Brassica rapa L. (syn. campestris)

Panigrahi

Patnaik²,

Kole³

et al. 2009

Zeitschrift Für Naturforschung C

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Genetic linkage analysis of 151 restriction fragment length polymorphism (RFLP) loci, that included eight new loci, detected by the six probes in the present study, and four trait loci including seed colour, leaf pubescence, resistance to white rust caused by Albugo candida race-2 (AC-2) and race-7 (AC-7) employing the MAPMAKER/EXP 3.0 programme led to the development of 10 linkage groups (LGs) spanning over 44.4 centiMorgan (cM) to 130.4 cM containing 9 to 22 loci and two short LGs with two or three marker loci in Brassica rapa. The enriched map covers 993.1 cM of B. rapa genome with an average marker interval of 6.41. Eight new RFLP loci occupied new map positions on fi ve linkage groups, LG 2, 3, 6, 8 and 9. Addition of these RFLP loci led to appreciable changes in the corresponding linkage groups and resulted in an increase of the total map length by 102.8 cM and of the marker interval by 0.35 cM. Interval mapping by using the computer programme MAPMAKER/ QTL 1.1 for scanning the genetic map led to the detection of one major quantitative trait locus (QTL) in LG 4 and one minor QTL in LG 8 governing resistance to AC-7. Both QTLs contributed 7.89 to the interaction phenotype (IP) score with 96.3% genetic variation. The multi-locus model suggested additive gene action with 96.8% genetic variation.

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Identification of a RAPD marker for palmitic-acid concentration in the seed oil of spring turnip rape (Brassica rapa ssp. oleifera)

Cited by 24 publications

References 10 publications

Sequence variations in the FAD2 gene in seeded pumpkins

Sequence variations in the FAD2 gene in seeded pumpkins

RFLP mapping of loci controlling self-incompatibility in Brassica campestris and their comparative mapping with B. napus and B. oleracea

Addition of Restriction Fragment Length Polymorphism Markers to the Genetic Linkage Map of Brassica rapa L. (syn. campestris)

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