Identification of a sequence characterized amplified region (SCAR) marker linked to the Puccinia psidii resistance gene 1 (Ppr1) in Eucalyptus grandis

Laia, Marcelo Luiz de; Alfenas, Acelino C.; Brommonschenkel, Sérgio Hermínio; Oda, Shinitiro; Mello, Eduardo; Silva, Inae Marie de Araujo; Gonçalves, Janaína Fernandes; Ferreira, Marcele dos Santos

doi:10.5897/ajar2013.8016

“…Rust, caused by the biotrophic fungus Austropuccinia psidii (A. psidii), is one of the most harmful diseases affecting Eucalyptus plantations, and consequently, considerably reduces their productivity, particularly in the first 2 years of plant development (Takahashi, 2002). Since E. grandis is commonly susceptible to pathogens, geneticists and breeders have used genomic tools applied to conventional breeding to select new rust-resistant genotypes and enhance the development of the next generation trees (Zamprogno et al, 2008;Boava et al, 2010;Silva et al, 2013;Laia et al, 2015;Butler et al, 2016). However, the antagonist bottlenecks have led researchers to question which genes are expressed and translated into functional proteins (Walley et al, 2013;Jafari et al, 2017;Yewdell and Chaudhuri, 2017).…”

Section: Introductionmentioning

confidence: 99%

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

Sekiya

¹

,

Marques

²

,

Leite

³

et al. 2021

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Eucalyptus rust is caused by the biotrophic fungus, Austropuccinia psidii, which affects commercial plantations of Eucalyptus, a major raw material for the pulp and paper industry in Brazil. In this manuscript we aimed to uncover the molecular mechanisms involved in rust resistance and susceptibility in Eucalyptus grandis. Epifluorescence microscopy was used to follow the fungus development inside the leaves of two contrasting half-sibling genotypes (rust-resistance and rust-susceptible), and also determine the comparative time-course of changes in metabolites and proteins in plants inoculated with rust. Within 24 h of complete fungal invasion, the analysis of 709 metabolomic features showed the suppression of many metabolites 6 h after inoculation (hai) in the rust-resistant genotype, with responses being induced after 12 hai. In contrast, the rust-susceptible genotype displayed more induced metabolites from 0 to 18 hai time-points, but a strong suppression occurred at 24 hai. Multivariate analyses of genotypes and time points were used to select 16 differential metabolites mostly classified as phenylpropanoid-related compounds. Applying the Weighted Gene Co-Expression Network Analysis (WGCNA), rust-resistant and rust-susceptible genotypes had, respectively, 871 and 852 proteins grouped into 5 and 6 modules, of which 5 and 4 of them were significantly correlated to the selected metabolites. Functional analyses revealed roles for photosynthesis and oxidative-dependent responses leading to temporal activity of metabolites and related enzymes after 12 hai in rust-resistance; while the initial over-accumulation of those molecules and suppression of supporting mechanisms at 12 hai caused a lack of progressive metabolite-enzyme responses after 12 hai in rust-susceptible genotype. This study provides some insights on how E. grandis plants are functionally modulated to integrate secondary metabolites and related enzymes from phenylpropanoid pathway and lead to temporal divergences of resistance and susceptibility responses to rust.

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“…Rust, caused by the biotrophic fungus Austropuccinia psidii (A. psidii), is one of the most harmful diseases affecting Eucalyptus plantations, and consequently, considerably reduces their productivity, particularly in the first 2 years of plant development (Takahashi, 2002). Since E. grandis is commonly susceptible to pathogens, geneticists and breeders have used genomic tools applied to conventional breeding to select new rust-resistant genotypes and enhance the development of the next generation trees (Zamprogno et al, 2008;Boava et al, 2010;Silva et al, 2013;Laia et al, 2015;Butler et al, 2016). However, the antagonist bottlenecks have led researchers to question which genes are expressed and translated into functional proteins (Walley et al, 2013;Jafari et al, 2017;Yewdell and Chaudhuri, 2017).…”

Section: Introductionmentioning

confidence: 99%

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“…Rust, caused by the biotrophic fungus Austropuccinia psidii (A. psidii), is one of the most harmful diseases affecting Eucalyptus plantations, and consequently, considerably reduces their productivity, particularly in the first 2 years of plant development (Takahashi, 2002). Since E. grandis is commonly susceptible to pathogens, geneticists and breeders have used genomic tools applied to conventional breeding to select new rust-resistant genotypes and enhance the development of the next generation trees (Zamprogno et al, 2008;Boava et al, 2010;Silva et al, 2013;Laia et al, 2015;Butler et al, 2016). However, the antagonist bottlenecks have led researchers to question which genes are expressed and translated into functional proteins (Walley et al, 2013;Jafari et al, 2017;Yewdell and Chaudhuri, 2017).…”

Section: Introductionmentioning

confidence: 99%

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0

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Identification of a sequence characterized amplified region (SCAR) marker linked to the Puccinia psidii resistance gene 1 (Ppr1) in Eucalyptus grandis

Cited by 3 publications

References 26 publications

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

Network Analysis Combining Proteomics and Metabolomics Reveals New Insights Into Early Responses of Eucalyptus grandis During Rust Infection

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Table_9.XLSX

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