Identification of a Transferable Linear Plasmid Carrying the Macrolide-Clindamycin Resistance Gene erm (X) in a Cutibacterium acnes Isolate from a Patient with Acne Vulgaris in Japan

Abstract

Cutibacterium acnes , one of the common skin bacteria, is known to exacerbate acne vulgaris. Macrolide-clindamycin-resistant C. acnes strains have been reported worldwide. In this study, we found a transferable linear plasmid carrying the macrolide-clindamycin resistance gene erm (X) in a C. acnes strain isolated from a patient with acne vulgaris.

First case of Cutibacterium avidum-infected pelvic lymphocele post-lymphadenectomy for endometrial cancer: A case report

“…However, two recent studies have reported the antimicrobial resistance of C. avidum . One study reported that erm(X) can be transmitted between C. acnes and C. avidum via Tn5432, which leads to both macrolide and clindamycin resistance [ 25 ]. Another study reported that a C. avidum strain acquired resistance to rifampin and levofloxacin during the treatment of orthopedic device-related infection [ 26 ].…”

Multidrug Resistance Plasmid pTZC1 Could Be Pooled among Cutibacterium Strains on the Skin Surface

“…qPCR was performed to determine the copy number of pTZC1. Genomic DNA was extracted as previously described ( 15 ). The copy number was analyzed using real-time PCR and Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan).…”

“…Mutations in 23S rRNA and acquisition of ribosomal methylase coding genes erm (X) and erm (50) are well-known mechanisms of resistance against the macrolides-clindamycin ( 12 , 13 ). erm (X) is found on chromosomes or linear plasmids and is transmitted between C. acnes strains ( 14 , 15 ). In contrast, erm (50) and the tetracycline resistance gene tet (W) are only found in C. acnes and are located on pTZC1.…”

Emergence of multidrug-resistant Pandoraea sputorum in Japan

“…As a breakpoint is not listed for Pandoraea species, it’s for B. cepacia were used. Genomic DNA was extracted from a single colony on MHA using phenol-chloroform-isoamyl alcohol (25:24:1) as previously described ( 5 ). Whole-genome sequencing was performed using Sequel IIe (Pacific Biosciences [PacBio], USA).…”