Identification of Active Components and Antifungal Activities of Extracts from Carpesium abrotanoides L. (Asteraceae)

Abstract

The target-directed efficient extraction and antifungal activities of extracts against seven phytopathogenic fungi from flowers, leaves and seeds of Carpesium abrotanoides L. (Asteraceae) were evaluated. In the seven tested fungi, the extracts of three parts of this plant produced by petroleum ether and chloroform, as well as extracts of flower and seed produced by ethyl acetate, had strong activities against Gibberella zeae, Phytophthora capsica and Rhizoctonia cerealis. The seed extract produced by chloroform was further fractionated to seven fractions [SS2(1–7)] using bioautography-guided TLC and its high active fraction (SS4) was identified using ultra-performance liquid chromatography–mass spectrometry. Eight compounds with higher contents in SS2-4 were identified. Among them, dehydrocostus lactone, isoalantolactone, parthenolide and alantolactone showed different concentration-dependent antifungal effects on R. cerealis, resulting in EC50s of 7.05, 18.22, 21.22 and 125.10 mg/L, respectively.