Identification of alternatively spliced isoforms of interleukin-2/15 receptor β chain in ducks

Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection

“…Fifty two-week-old ducks were infected intramuscularly with 5 Â 10 7 CFU of R. anatipestifer serotype 7 in 200 ml phosphate buffer saline (PBS). The bacterium used in this study (Jeong et al, 2014) was isolated from a commercial duck farm in Changwon, Gyeongnam, South Korea and serotyped in Chonbuk National University. The isolate was grown in sheep blood agar plates (Asan Pharmaceutical, Korea) at 37 C in 5% CO 2 .…”

Untitled

“…The bacterium used in this study, R. anatipestifer serotype 7, was isolated from a commercial duck farm in Changwon, Gyeongnam Province, Korea, and serotyped at Chonbuk National University [40]. The isolate was grown on blood agar plates with 5% sheep blood (Asan Pharmaceutical, Korea), and a single colony was then cultivated in tryptic soy broth (BD Difco, USA) at 37˚C with vigorous shaking, as previously described [15,40]. Viable bacterial counts for the final challenge concentrations were determined by plating serial dilutions (10-fold) onto 5% sheep blood agar plates.…”

Untitled

“…The birds were randomly assigned to four groups (n = 25/group) and housed in separate buildings: one group consisted of non-infected and untreated control birds; one group consisted of non-infected/DIM-treated birds; one group consisted of infected and untreated birds and one group consisted of infected/DIMtreated birds. The bacterium used in this study, R. anatipestifer serotype 7, was isolated from a commercial duck farm in Changwon, Gyeongnam Province, Korea, and serotyped at Chonbuk National University [40]. The isolate was grown on blood agar plates with 5% sheep blood (Asan Pharmaceutical, Korea), and a single colony was then cultivated in tryptic soy broth (BD Difco, USA) at 37˚C with vigorous shaking, as previously described [15,40].…”