Identification of an S 5 n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

Wei, ChangMin; Wang, Lan; Yang, Youxin; Chen, Zhixiong; Shahid, Muhammad Qasim; Li, Jinquan; Liu, Xiangdong; Lu, Yong-gen

doi:10.1007/s11434-010-0154-y

Cited by 13 publications

(13 citation statements)

References 17 publications

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“…S 5 n distribution rates in indica and japonica subspecies are almost identical [10]. S 5 n was also found in common wild rice (O. rufipogon) indigenous to China, including those in Guangdong, Guangxi, Jiangxi, Hainan and Hunan provinces or Autonomous region [11,[18][19][20]. Moreover, S 5 n was also found in O. nivara, which is closely related to O. rufipogon.…”

Section: S 5 N Evolutionmentioning

confidence: 99%

“…More than 300 different rice varieties (lines) carrying S 5 n have been identified, including indica and japonica, since Ikehashi and Araki proposed the "wide compatibility gene (S 5 n ) theory" in 1986 [10,11,17]. S 5 n distribution rates in indica and japonica subspecies are almost identical [10].…”

Section: S 5 N Evolutionmentioning

confidence: 99%

“…We designed S 5 n functional molecular markers based on the sequence of S 5 n and identified 48 new germplasms with S 5 n from the Chinese rice core collection and wild rice [10,11]. In the present study, DNA of the 48 lines containing S 5 n was fully sequenced.…”

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confidence: 99%

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Molecular evolution of rice S 5 n and functional comparison among different sequences

Tong

Yang

et al. 2011

Chin. Sci. Bull.

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The wide compatibility gene, S 5 n , can overcome embryo sac sterility between indica and japonica subspecies of rice. Therefore, it is very important to characterize the features of the S 5 n sequence to reveal the origin and evolution of S 5 n . In this paper, 26 cultivated rice haplotypes and 22 wild rice accessions harboring S 5 n were used to sequence S 5 n . The results showed that 15 genotypes among the 48 materials were fully consistent with control cultivar 02428 (CK). The other 33 accessions had different degrees of variation in the S 5 n sequence. Variations in the coding region mainly occurred in the second exon and eight materials showed a 10-bp deletion at 1710-1719 bp, including wild (O. nivara) and cultivated rice, such as IRW501 and Yuetai B. S 5 n sequences were not biased and evolved neutrally. The 48 materials could be divided into 4 categories using a phylogenetic tree of the amino acid sequences. Most of the wild rice clustered together, and the cultivated rice clustered into another group. Eight cultivated rice and O. nivara (wild rice) clustered in another group, which were found to lack 10 consecutive bases in exon 2. Eight rice varieties with high numbers of differences in their S 5 n coding regions were crossed with testers (typically indica and japonica) to produced test cross F 1 populations. The F 1 s were examined for their ability to overcome indica-japonica hybrid sterility. The result showed that the embryo sac fertility of S 5 n -containing hybrids increased significantly compared with control hybrids, but there were no differences among the materials with divergent sequences, indirectly proving that S 5 n is a non-functional gene. rice (Oryza sativa L.), hybrid sterility, S 5 n , wide compatibility gene, O. ruvfipogon, O. nivara Citation: Tong J F, Li Y H, Yang Y X, et al. Molecular evolution of rice S 5 n and functional comparison among different sequences.

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Section: S 5 N Evolutionmentioning

confidence: 99%

Section: S 5 N Evolutionmentioning

confidence: 99%

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Molecular evolution of rice S 5 n and functional comparison among different sequences

Tong

Yang

et al. 2011

Chin. Sci. Bull.

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“…The genus Oryza contains 20 wild rice species, which are classified into 10 distinct genome types (AA, BB, CC, EE, FF, GG, BBCC, CCDD, HHJJ, and HHKK). Wild rice has many valuable features, including tolerance to different biotic and abiotic stressors; therefore, wild rice is considered a good genetic resource for rice improvement (Zhong et al, 1995;Wei et al, 2010;Liu et al, 2011;Tong et al, 2011). O. officinalis (2n = 24, CC) was originally distributed throughout South China, and is a perennial species with resistance to bacterial blight, rice blast, the brown planthopper (Nilaparvata lugens), and leaf rollers, is salt-and cold-tolerant, and has a high protein content, so is an important germplasm for rice breeding (Zhong et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone

Liu¹,

Zhang²,

Chen³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was selected as a probe to screen the O. officinalis genomic transformation-competent artificial chromosome library by Southern blot; 11 positive transformation-competent artificial chromosome clones were subsequently detected. By Agrobacterium-mediated transformation, an indica rice variety, Huajingxian 74 (HJX74), was transformed with a TAC clone harboring a NAC gene-positive genomic fragment from O. officinalis. Molecular analysis revealed that the O. officinalis genomic fragment was integrated into the genome of HJX74. The transgenic lines exhibited high tolerance to drought stress. Our results demonstrate that the introduction of stress-related transformation-competent artificial chromosome clones, coupled with a transgenic validation approach, is an 13667-13678 (2015) effective method of transferring agronomically important genes from O. officinalis to cultivated rice.

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“…Our lab designed S 5 n functional markers S5‐t1 and S5‐t2 according to the deletion of S 5 n sequence and revealed that 8 genotypes containing the S 5 n gene (Yang et al, 2009a) and 18 accessions of Oryza rufipogon carried the S 5 n allele in the heterozygous state ( S 5 n S 5 i/j ) (Wei et al, 2010). Yang et al (2009b) also developed the S 5 n functional marker S5136 based on the deletion of the DNA sequence of S 5 n and identified 13 rice germplasm with the S 5 n gene.…”

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confidence: 99%

Wide‐Compatibility Gene S₅ⁿ Exploited by Functional Molecular Markers and Its Effect on Fertility of Intersubspecific Rice Hybrids

Yang

Tong

et al. 2012

Crop Science

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Embryo-sac abortion is one of the nnost important reasons for hybrid sterility between indica and japónica subspecies. Wide compatibility gene Sg" can overcome the embryo-sac sterility between indica-japonica hybrids caused by the Sj locus located on chromosome 6. In this paper, key sequence analysis and functional molecular markers were used to identify Sj" gene-harboring genotypes from 646 rice {Oryza sativa L.) genotypes, including 323 cultivated and 323 wild rice genotypes. Whole-mount eosin Bstaining confocal laser scanning microscopy was employed to observe the embryo-sac fertility of hybrids. A total of 28 genotypes, including 16 cultivated, 9 Oryza rufipogon accessions, and 3 Oryza nivara accessions, were found to have the S^" gene. These results suggested that the Sj" gene differentiated and evolved from wild rice. Different indica-japonica hybrids were developed using O. sativa genotypes carrying the Sj" gene and without the S^" gene. Embryosac fertility was more than 93% in the Sg" geneharboring hybrids, whereas embryo-sac fertility was relatively low in control hybrids between typical indica and japónica cultivars without the Sj" gene, suggesting that Sg" can overcome the sterility between indica-japonica hybrids. Furthermore, it verified the reliability of molecular functional markers to detect Sg" gene-harboring genotypes. Functional molecular markers used in the present study will be very useful to identify Sg" gene-harboring genotypes, and genotypes detected with the Sg" gene in the present study are very important germplasm for overcoming intersubspecific hybrid sterility.

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Identification of an S 5 n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

Cited by 13 publications

References 17 publications

Molecular evolution of rice S 5 n and functional comparison among different sequences

Molecular evolution of rice S 5 n and functional comparison among different sequences

Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone

Wide‐Compatibility Gene S₅ⁿ Exploited by Functional Molecular Markers and Its Effect on Fertility of Intersubspecific Rice Hybrids

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Identification of an S 5 n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

Cited by 13 publications

References 17 publications

Molecular evolution of rice S 5 n and functional comparison among different sequences

Molecular evolution of rice S 5 n and functional comparison among different sequences

Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone

Wide‐Compatibility Gene S5n Exploited by Functional Molecular Markers and Its Effect on Fertility of Intersubspecific Rice Hybrids

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Wide‐Compatibility Gene S₅ⁿ Exploited by Functional Molecular Markers and Its Effect on Fertility of Intersubspecific Rice Hybrids