Identification of B-cell Epitopes on the S4 Subunit of Pertussis Toxin

Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine

“…The plates were washed, and samples diluted in PBS-Brij 35 plus 0.02% bovine serum albumin were added to the fetuin-coated plates and incubated for 1 h at RT. After incubation, plates were washed and reacted with a monoclonal antibody to Ptx (19,20,44) for 2 h at RT. The plates were washed and the secondary antibodies, alkaline phosphatase-conjugated goat anti-mouse immunoglobulin G (IgG) and IgM (Tago, Burlingame, Calif.) diluted 1:3,000 in PBSBrij, were added to the plate and incubated for 2 h at RT.…”