Identification of B-cell epitopes on structural proteins VP1 and VP2 of Senecavirus A and development of a multi-epitope recombinant protein vaccine

Zhang, Zhongwang; Yao, Fei; Lv, Jianliang; Ding, Yongsheng; Liu, Xinsheng; Zhang, Liping; Ma, Zhongyuan; Zhou, Peng; Wang, Yonglu; Guo, Huichen; Pan, Li

doi:10.1016/j.virol.2023.03.015

Cited by 6 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secondly, by targeting the production of the epitope directly within the host cells, gene therapy circumvents the need for external administration of epitopes, making it a potentially more convenient and controlled approach. The recognition of antigenic epitopes holds the utmost significance in the advancement of vaccine development [ 64 , 65 , 66 ]. These epitopes serve as the building blocks for designing vaccines that activate the immune system to generate antibodies capable of recognizing and neutralizing specific pathogens.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens

Palma¹

2023

Vaccines

View full text Add to dashboard Cite

Traditional vaccines use inactivated or weakened forms of pathogens which could have side effects and inadequate immune responses. To overcome these challenges, phage display has emerged as a valuable tool for identifying specific epitopes that could be used in vaccines. This review emphasizes the direct connection between epitope identification and vaccine development, filling a crucial gap in the field. This technique allows vaccines to be engineered to effectively stimulate the immune system by presenting carefully selected epitopes. Phage display involves screening libraries of random peptides or gene/genome fragments using serum samples from infected, convalescent, or vaccinated individuals. This method has been used to identify epitopes from various pathogens including SARS-CoV-2, Mycobacterium tuberculosis, hepatitis viruses, H5N1, HIV-1, Human T-lymphotropic virus 1, Plasmodium falciparum, Trypanosoma cruzi, and Dirofilaria repens. Bacteriophages offer advantages such as being immunogenic carriers, low production costs, and customization options, making them a promising alternative to traditional vaccines. The purpose of this study has been to highlight an approach that encompasses the entire process from epitope identification to vaccine production using a single technique, without requiring additional manipulation. Unlike conventional methods, phage display demonstrates exceptional efficiency and speed, which could provide significant advantages in critical scenarios such as pandemics.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The recognition of antigenic epitopes holds the utmost significance in the advancement of vaccine development [ 64 , 65 , 66 ]. These epitopes serve as the building blocks for designing vaccines that activate the immune system to generate antibodies capable of recognizing and neutralizing specific pathogens.…”

Section: Resultsmentioning

confidence: 99%

Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens

Palma¹

2023

Vaccines

View full text Add to dashboard Cite

show abstract

“…To date, several antigenic epitopes of SVA VP2 have been identified through bioinformatics prediction and synthetic polypeptide therapy using mAbs or porcine serum (Fan et al, 2020 ; Ru et al, 2023 ; Zhang et al, 2023 ). The 141 LDVRPDGKAKSLEELNEEQW 160 region is a dominant epitope region with relatively abundant epitopes in the VP2 protein (Fan et al, 2020 ; Wen et al, 2022 ; Ru et al, 2023 ; Zhang et al, 2023 ). In addition, Wen et al obtained five neutralizing mAbs by immunizing mice with ultra-purified SVA, and the linear epitope 153 QELNEE 158 recognized them.…”

Section: Discussionmentioning

confidence: 99%

“…In prior studies, several B-cell epitopes (BCEs) of SVA VP2 were identified by bioinformatics prediction (Ru et al, 2023 ; Zhang et al, 2023 ), overlapping synthesis of polypeptides (Ma et al, 2022 ) and monoclonal antibodies (mAbs) (Fan et al, 2020 ; Wen et al, 2022 ). It has been confirmed that 153 QELNEE 158 is a conserved antigenic epitope of the VP2 protein and has virus-neutralizing activity (Wen et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of a linear B-cell epitope on the “puff” loop of the Senecavirus A VP2 protein involved in receptor binding

Zhou,

Sun,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Senecavirus A (SVA) is an important emerging swine pathogen that causes vesicular lesions in swine and acute death in newborn piglets. VP2 plays a significant role in the production of antibodies, which can be used in development of diagnostic tools and vaccines. Herein, the aim of the current study was to identify B-cell epitopes (BCEs) of SVA for generation of epitope-based SVA marker vaccine. Three monoclonal antibodies (mAbs), named 2E4, 1B8, and 2C7, against the SVA VP2 protein were obtained, and two novel linear BCEs, 177SLGTYYR183 and 266SPYFNGL272, were identified by peptide scanning. The epitope 177SLGTYYR183 was recognized by the mAb 1B8 and was fully exposed on the VP2 surface, and alanine scanning analysis revealed that it contained a high continuity of key amino acids. Importantly, we confirmed that 177SLGTYYR183 locates on “the puff” region within the VP2 EF loop, and contains three key amino acid residues involved in receptor binding. Moreover, a single mutation, Y182A, blocked the interaction of the mutant virus with the mAb 1B8, indicating that this mutation is the pivotal point for antibody recognition. In summary, the BCEs that identified in this study could be used to develop diagnostic tools and an epitope-based SVA marker vaccine.

show abstract

A nanoparticle vaccine based on the VP121–26 and VP2 structural proteins of Senecavirus A induces robust protective immune responses

Cao,

Li,

Zhang

et al. 2024

Veterinary Microbiology

View full text Add to dashboard Cite

Identification of B-cell epitopes on structural proteins VP1 and VP2 of Senecavirus A and development of a multi-epitope recombinant protein vaccine

Cited by 6 publications

References 27 publications

Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens

Epitopes and Mimotopes Identification Using Phage Display for Vaccine Development against Infectious Pathogens

Identification of a linear B-cell epitope on the “puff” loop of the Senecavirus A VP2 protein involved in receptor binding

A nanoparticle vaccine based on the VP121–26 and VP2 structural proteins of Senecavirus A induces robust protective immune responses

Contact Info

Product

Resources

About