Identification of Bacillus Strains Using the API System

Logan, Niall A.; Berkeley, R. C. W.

doi:10.1099/00221287-130-7-1871

Cited by 289 publications

(289 citation statements)

References 17 publications

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“…Isolate Dac521, a Gram-positive, sporulating, nonmotile, rod-shaped organism (2.0 x 0.7 pm) which grew between 35 and 70 "C on solid media and up to 73 "C in liquid media, was identified as a thermophilic Bacillus species using the API 5OCH and 20E identification test strips (Logan & Berkeley, 1984). On nutrient agar plates, Dac521 colonies were of non-uniform size, circular form, convex elevation, with an entire margin and a yellow/grey colour.…”

Section: Morphology and Taxonomymentioning

confidence: 99%

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Novel thermophilic bacteria producing nitrile-degrading enzymes

1997

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Summary: The first known report of the isolation of thermophilic bacteria which produce nitrile-degrading enzymes is presented. One of the strains isolated was studied in detail. Strain Dac521, classified as Bacillus pallidus, was capable of growth on acetonitrile, benzonitrile, propionitrile, acetamide, benzamide and propionamide as the sole carbon and nitrogen source in minimal nutrient media. The strain produced separate aliphatic-nitrile (e.g. acetonitrile)- and aromatic-nitrile (e.g. benzonitrile)-degrading activities. Acetonitrile-degrading activity was produced constitutively and enzyme production was not enhanced by the addition of substrate. Under conditions where benzonitrile was the sole carbon and nitrogen source in minimal nutrient media, acetonitrile-degrading enzyme activity was completely inhibited and benzonitrile-degrading activity was induced. Growth on substrates as sole carbon and nitrogen sources, together with the substrate specificity of cell-free extracts, suggested that acetonitrile and benzonitrile degradation may have occurred via nitrile hydratase and nitrilase pathways, respectively. Both the acetonitrile- and benzonitrile-degrading enzyme systems were significantly more thermostable in whole-cell preparations and cell-free extracts compared to their mesophilic counterparts.

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Section: Morphology and Taxonomymentioning

confidence: 99%

“…Analytical profile index (API) tests 5OCH and 20E (Logan & Berkeley, 1984) were used to aid identification. API 50 CHB defined medium and distilled water were used to inoculate the test kits, respectively.…”

Section: Introductionmentioning

confidence: 99%

Novel thermophilic bacteria producing nitrile-degrading enzymes

1997

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“…controls. API tests (bioMérieux) were carried out as in Logan & Berkeley (1984), except that, with API 50CHB tests, results were also recorded after 4 days. All tests were replicated at least twice.…”

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confidence: 99%

Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.

Enright

McInerney

Griffin

2003

International Journal of Systematic and Evolutionary Microbiology

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NoteCharacterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov. Endospore-forming bacteria were isolated from insect-pathogenic nematodes, Heterorhabditis spp., from three diverse geographical locations. Spindle-shaped sporangia of these bacteria adhere to the free-living infective stage of the nematode, which carries them to new insect hosts, where the bacterium reproduces. These isolates were characterized based on phenotypic and chemotaxonomic properties and 16S rRNA gene sequences. Analysis of the 16S rRNA gene placed the isolates within the genus Paenibacillus. The isolates shared higher sequence similarities with each other (95?1-100 %) than they did with any other named species within the genus (89?2-94 %). Paenibacillus macquariensis, Paenibacillus azoreducens, Paenibacillus amylolyticus and Paenibacillus durus were among the species with highest sequence similarity to these isolates. The isolates shared a high degree of phenotypic similarity and were easily distinguished from closely related members of the genus. Anteiso-C 15 : 0 and C 16 : 0 were among the major fatty acid types and the DNA G+C content was approximately 44 mol% in all isolates. DNA-DNA similarity studies revealed genomic heterogeneity among the isolates, such that they are likely to represent more than one species. Two of the isolates (both from a Heterorhabditis megidis isolate from Estonia) are phenotypically distinguishable from the others and are proposed as a single species, Paenibacillus nematophilus sp. nov. The type strain for this novel species is NEM1a T (=DSM 13559 T =NCIMB 13845 T ). The other isolates, although closely related to the proposed species, are likely to represent at least one, but most likely two, novel species.The genus Paenibacillus includes several species that are associated with dead insects in one way or another. Amongst these are obligate insect pathogens such as Paenibacillus popilliae, Paenibacillus lentimorbus and Paenibacillus larvae (Pettersson et al., 1999), as well as non-pathogens/facultative pathogens such as Paenibacillus apiarus (Nakamura, 1996) and Paenibacillus alvei (Krieg, 1981). Enright et al. (2001) found a Paenibacillus sp. associated with an insectpathogenic nematode, Heterorhabditis megidis EU17. This nematode was isolated during a survey conducted in Estonia (Griffin et al., 1999).Heterorhabditis spp. nematodes are lethal pathogens of insects. Infective juveniles (IJs), the only infective stage of these nematodes, carry in their intestine a symbiotic bacterium belonging to the genus Photorhabdus (Forst et al., 1997). Upon infection of a suitable host insect, the symbiont is released into the haemocoel and causes the death of the insect within 48 h (Poinar, 1990). The Photorhabdus sp. then completely colonizes the dead insect cadaver, assisting in the breakdown of the insect tissues by secretion of an array of enzymes (Forst & Nealson, 1996). It is upon this bacterium and the ...

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“…The API 20E, API 20NE, API Staph and API 50CH were designed for identifying members of the Enterobacteriaceae, Nonenteric Gram-negative rods; Staphylococcus and Bacillus respectively. APIWEB software was used for identification and was considered acceptable when given a probability of 80% or greater (BioMerieux, France) (Awong-Taylor et al, 2008;Logan and Berkeley, 1984).…”

Section: Characterization Of Isolatesmentioning

confidence: 99%

Diversity of culturable aerobic bacteria colonizing four petroleum by-products storage reservoirs

Saiuml¹,

Hamed²,

Gam³

et al. 2013

Afr. J. Microbiol. Res.

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The petroleum reservoirs are commonly exposed to bacterial contamination. The proliferation of several bacterial species, could modify the product quality, change viscosity, help other sulfatereducing, iron reducing bacteria to grow, and accelerate reservoir biocorrosion. Thus, companies of storage and transport of petroleum frequently use biocides to prohibit bacterial proliferation. However, the use of the appropriate biocide, need an obvious knowledge of bacterial population harbouring the reservoir. Thus, in this study, we intended to isolate and identify the aerobic bacterial population existing in different reservoirs of petroleum derivatives in the refinery of Bizerte, Tunisia. The isolation and identification of these microorganisms may be of great interest in bioremediation of polluted sites. Besides, it could inform about hydrocarbon specificity of these microorganisms and therefore lead to the use of suitable biocides to clean these storage reservoirs. 25 different bacterial strains were isolated from water collected from reservoirs of crude oil, gasoline, diesel oil and kerosene. The different strains were identified using morphological and biochemical characterization, as well as sequencing of 16S rRNA. The systematic study of these strains showed that they belong to six different genera. The highest strain number was affiliated to the genera of Pseudomonas and Bacillus followed by genera of Staphylococcus, Enterobacter Comamonas and Paenibacillus. The results of this study are discussed in terms of the ecological significance of these microorganisms in relation with physicochemical conditions of reservoirs and hydrocarbon composition.

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Identification of Bacillus Strains Using the API System

Cited by 289 publications

References 17 publications

Novel thermophilic bacteria producing nitrile-degrading enzymes

Novel thermophilic bacteria producing nitrile-degrading enzymes

Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.

Diversity of culturable aerobic bacteria colonizing four petroleum by-products storage reservoirs

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