Identification of Candida spp. isolated from vaginal swab by phenotypic methods and multiplex PCR in Duhok, Iraq

Mohammed, Ahmed Basheer; Ali, Jumma Hussein; Abdullah, Samir K.

doi:10.18203/2320-6012.ijrms20151165

Cited by 5 publications

(6 citation statements)

References 16 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies have reported lower rates of C. glabrata compared to C. krusei [26,32], C. tropicalis [3,5] and C. parapsilosis [12], while our results and other studies in Iraq and Egypt [15,17] demonstrated C. glabrata as the most prevalent species in diabetic and non-diabetic women. High prevalence of C. glabrata was also reported in other countries among diabetic [16,29,30] and non-diabetic women [25,27,32], which indicate an increasing trend in the prevalence of infections caused by this species.…”

Section: Discussionsupporting

confidence: 53%

“…However, in a similar study in Sari (Iran), the frequency of NAC species in VVC group was higher than RVVC group [12]. Other reports from Nigeria [13,15], India [3,25,26], Iraq [17], and Burkina Faso of Africa [27] also revealed a higher prevalence of NAC species compare to C. albicans among diabetic and non-diabetic women. On the contrary, in diabetic patients of Yemen [29], Kermanshah (Iran) [30], and some non-diabetic patients with VVC in Iran and other countries, the prevalence of NAC species was less than C. albicans [10,20,25,31,32].…”

Section: Discussionmentioning

confidence: 68%

“…increase. Some studies have estimated that %15 to %47 of RVVC cases were caused by C. glabrata, which is a major cause of treatment-resistant severe infections worldwide [11,12,17].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Investigating the Frequency of Candida glabrata in Diabetic Women of Tehran with Recurrent and Non-recurrent Vulvovaginal Candidiasis Using PCR-RFLP Assay

Paknejadi

Bayat

Razavilar

2019

JoMMID

View full text Add to dashboard Cite

INTRODUCTION Vaginitis is one of the most prevalent reproductive tract infections (RTIs) among sexually active women. Annually, about 340 million cases of curable sexually transmitted infections (STIs) occur worldwide, mostly in developing countries [1, 2]. After bacterial infections, vulvovaginal candidiasis is believed to be responsible for approximately one-third of vaginitis cases [3, 4]. However, some studies indicate a higher prevalence of Candida infection compared to bacterial vaginosis and trichomoniasis [5, 6, 7]. About %75 of healthy women within the age range of 25-50 years have experienced non-recurrent vulvovaginal candidiasis (VVC) at least once in their lifetime, but 5% to 20% are prone to recurrent vulvovaginal candidiasis (RVVC) at least four times during a year [7-9]. Candida species are part of normal flora in the skin, mucous membranes, and gastrointestinal tract; however, in the presence of predisposing factors, they can appear as an opportunistic agent causing severe infections. The most critical predisposing factors include immunosuppressive diseases, uncontrolled diabetes, high carbohydrate diet, pregnancy, lack of personal hygiene, estrogen contraceptives, high sexual activity or high-risk behaviors, prescription of improper drug, self-treatment and prolonged use of broad-spectrum antibiotics and corticosteroids, incomplete or extended use of antimycotics, and resistance of Candida species to azoles [10-13]. Diabetes mellitus (DM) is one of the major risk factors for severe infections caused by different Candida species. Women with VVC and DM, particularly those with poorly controlled hyperglycemia, are prone to develop RVVC caused by nonalbicans Candida (NAC) species [14-16]. Non-recurrent vulvovaginal candidiasis is an uncomplicated and mild disease which is mainly prevalent among healthy women infected with Candida albicans, while recurrent VVC is a complicated and more severe disease often caused by drugresistant NAC species in immunocompromised women [14-16]. Candida albicans is known as the primary cause of vulvovaginal candidiasis, however; today, NAC species, especially C. glabrata, which is intrinsically resistant to a vast range of azoles and antifungal compounds are on the Introduction: Vulvovaginal Candidiasis (VVC) is one of the most common genital tract infections among women, especially in diabetic patients. The increasing prevalence of recurrent infections caused by drug-resistant non-albicans species necessitates further studies on diabetic patients and the identification of causative agents by reliable molecular techniques. The obtained results can assist in adopting proper treatment procedures and prevention of recurrent vulvovaginitis (RVVC). Methods: In a cross-sectional study, 150 vaginal discharge samples were collected from diabetic women suspected of candidiasis referring to health centers in Tehran province. Following the culture of samples on SDA, CHROMagar Candida and PCR-RFLP were used for presumptive and definitive identification of Candida species, respecti...

show abstract

Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 68%

See 1 more Smart Citation

Investigating the Frequency of Candida glabrata in Diabetic Women of Tehran with Recurrent and Non-recurrent Vulvovaginal Candidiasis Using PCR-RFLP Assay

Paknejadi

Bayat

Razavilar

2019

JoMMID

View full text Add to dashboard Cite

show abstract

“…4,20 For presumptive identification, Candida isolates were sub cultured onto chromogenic candida agar (Rapid labs Ltd,Essex,UK) which differentiate Candida species on the basis of colour change. 21 According to the manufacturer's instructions the colors of the colonies are as follows: Candida albicans (Emerald, with metallic shine), C. dublinensis (Dark green, matt), C. glabrata (White, shiny), C. krusei (Light pink), C. tropicalis (Red purple).…”

Section: Methodsmentioning

confidence: 99%

Multiplex polymerase chain reaction identification of Candida species colonized sputum of patients suffering from various respiratory tract disorders in Duhok, Iraq

Mohammed

Ali²,

Abdullah

2016

Int J Res Med Sci

Self Cite

View full text Add to dashboard Cite

“…The data about such a condition is sparse in our region. One study estimated that recurrent genital infection occurs in about 5% of women of reproductive age [ 21 ]. It was previously found that patient with genital infection is at high risk to contract other sexually transmitted diseases including HPV infection [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Human papillomavirus (HPV) genotype prevalence and impact of COVID-19 on the HPV prevention program in Duhok city

Daniel

Mohammed²,

Ibrahim³

et al. 2022

Dialogues in Health

View full text Add to dashboard Cite

Identification of Candida spp. isolated from vaginal swab by phenotypic methods and multiplex PCR in Duhok, Iraq

Cited by 5 publications

References 16 publications

Investigating the Frequency of Candida glabrata in Diabetic Women of Tehran with Recurrent and Non-recurrent Vulvovaginal Candidiasis Using PCR-RFLP Assay

Investigating the Frequency of Candida glabrata in Diabetic Women of Tehran with Recurrent and Non-recurrent Vulvovaginal Candidiasis Using PCR-RFLP Assay

Multiplex polymerase chain reaction identification of Candida species colonized sputum of patients suffering from various respiratory tract disorders in Duhok, Iraq

Human papillomavirus (HPV) genotype prevalence and impact of COVID-19 on the HPV prevention program in Duhok city

Contact Info

Product

Resources

About