Identification of CD4+ and CD8+ T cell epitopes of woodchuck hepatitis virus core and surface antigens in BALB/c mice

Ochoa-Callejero, Laura; Otano, Itziar; Vales, África; Olagüe, Cristina; Sarobe, Pablo; Lasarte, Juan José; Prieto, Jesús; Menne, Stephan; González‐Aseguinolaza, Gloria

doi:10.1016/j.vaccine.2010.05.043

Cited by 4 publications

(2 citation statements)

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“…Similar to our results for the HBcAg 64-72 peptide, a HBcAg peptide consisting of amino acid residues 63-71 (GELMTLATW) has been previously identified by Townsend et al as an epitope which could be recognized by rhesus monkey CTLs [55]. Additionally, another similar CTL epitope derived from woodchuck hepatitis virus (WHV) core antigen (WHV 61-69) has been identified recently by Ochoa-Callejero et al [56]. These results suggested that C 64-72 may be an immunodominant epitope of HBV core antigen capable of eliciting specific CTL responses.…”

Section: Discussionsupporting

confidence: 89%

Identification of HLA-A*0201-restricted CD8+ T-cell epitope C64–72 from hepatitis B virus core protein

Liu

Zheng

et al. 2012

International Immunopharmacology

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Section: Discussionsupporting

confidence: 89%

Identification of HLA-A*0201-restricted CD8+ T-cell epitope C64–72 from hepatitis B virus core protein

Liu

Zheng

et al. 2012

International Immunopharmacology

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“…4 It is worth noting that the antigenicity of preS2 is stronger than that of HBsAg, with a robust T and B cell recognition cluster. 5 During HBV infection, it can induce the host's immune response and produce preS2 antibodies, which provide an important defense function for eliminating HBV and preventing virus from invading normal liver cells. 6 Accordingly, preS2 antibody may be useful to inhibit the infection and duplication of HBV and exhibit antitumor activity in cancerous liver cells that persistently express HBx and preS2.…”

Section: Introductionmentioning

confidence: 99%

The Strategy of Conditionally Replicating Adenovirus-Mediated PreS2 Mini-Antibody Expression Has Dual Effects of Inhibiting HBV Infection and Preventing Hepatocellular Carcinoma

Zeng

et al. 2021

CMAR

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Aim To investigate the inhibitory effect of hepatitis B virus (HBV) preS2 mini-antibody (mPreS2) against HBV infection, HBV-associated liver injury and HBV-associated hepatic carcinogenesis. Methods A recombinant adenovirus vector with the human survivin promoter and mPreS2 gene, Ad5SVP-mPreS2, was constructed. Fluorescence microscopy examination and TCID 50 analysis were utilized to determine the specific proliferation of recombinant adenovirus in liver cancer cells. Western blot analysis was used to determine the mPreS2 expression levels. Enzyme-linked immunosorbent assay (ELISA) was used to examine HBsAg levels to evaluate the inhibitory effect of mPreS2 against HBV infection. The protective effects on hepatic function and preventive effects against hepatic carcinogenesis of Ad5SVP-mPreS2 were studied in diethylnitrosamine (DEN)-treated HBV transgenic Imprinting Control Region mice. Results The recombinant adenovirus regulated by the human survivin promoter proliferated exclusively in liver cancer cells rather than normal liver cells. The expression levels of mPreS2 were increased in liver cancer cells compared with normal liver cells, and mPreS2 could be used to recognize liver cells from HBV transgenic mice. ELISA showed that HBsAg levels were decreased in the group treated with Ad5SVP-mPreS2. Ad5SVP-mPreS2 had a protective effect on hepatic function in a DEN-induced liver injury model because of lower serum levels of alanine transaminase and aspartate transaminase. Additionally, HBV transgenic mice treated with Ad5SVP-mPreS2 had fewer and smaller cancerous nodes after induction with DEN than untreated mice. Conclusion Conditionally replicating adenovirus-mediated mPreS2 expression inhibited HBV infection and had an inhibitory effect on liver injury and hepatocellular carcinogenesis in HBV transgenic mice.

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Identification of H-2d Restricted T Cell Epitope of Foot-and-mouth Disease Virus Structural Protein VP1

Liu

Wang

Zhang

et al. 2011

Virol J

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BackgroundFoot-and-mouth disease (FMD) is a highly contagious and devastating disease affecting livestock that causes significant financial losses. Therefore, safer and more effective vaccines are required against Foot-and-mouth disease virus(FMDV). The purpose of this study is to screen and identify an H-2d restricted T cell epitope from the virus structural protein VP1, which is present with FMD. We therefore provide a method and basis for studying a specific FMDV T cell epitope.ResultsA codon-optimized expression method was adopted for effective expression of VP1 protein in colon bacillus. We used foot-and-mouth disease standard positive serum was used for Western blot detection of its immunogenicity. The VP1 protein was used for immunizing BALB/c mice, and spleen lymphocytes were isolated. Then, a common in vitro training stimulus was conducted for potential H-2Dd, H-2Kd and H-2Ld restricted T cell epitope on VP1 proteins that were predicted and synthesized by using a bioinformatics method. The H-2Kd restricted T cell epitope pK1 (AYHKGPFTRL) and the H-2Dd restricted T cell epitope pD7 (GFIMDRFVKI) were identified using lymphocyte proliferation assays and IFN-γ ELISPOT experiments.ConclusionsThe results of this study lay foundation for studying the FMDV immune process, vaccine development, among other things. These results also showed that, to identify viral T cell epitopes, the combined application of bioinformatics and molecular biology methods is effective.

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Identification of CD4+ and CD8+ T cell epitopes of woodchuck hepatitis virus core and surface antigens in BALB/c mice

Cited by 4 publications

References 38 publications

Identification of HLA-A*0201-restricted CD8+ T-cell epitope C64–72 from hepatitis B virus core protein

Identification of HLA-A*0201-restricted CD8+ T-cell epitope C64–72 from hepatitis B virus core protein

The Strategy of Conditionally Replicating Adenovirus-Mediated PreS2 Mini-Antibody Expression Has Dual Effects of Inhibiting HBV Infection and Preventing Hepatocellular Carcinoma

Identification of H-2d Restricted T Cell Epitope of Foot-and-mouth Disease Virus Structural Protein VP1

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