Identification of coding region SNPs from specific and sensitive mRNA biomarkers for the deconvolution of the semen donor in a body fluid mixture

Liu, Jinding; Cheng, Xiaojuan; Liu, Feng; Tang, Hao; Wang, Jiaqi; Guo, Jiangling; Li, Jintao; Liu, Zidong; Li, Wenyan; Shi, Jie; Zhang, Xiuying; Li, Jing; Yan, Jiangwei; Zhang, Gengqian

doi:10.1016/j.fsigen.2021.102483

Cited by 15 publications

(2 citation statements)

References 44 publications

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“…A number of approaches are being taken to improve the sensitivity and specificity of BFID in recent years including DNA methylation [ [148] , [149] , [150] , [151] , [152] , [153] , [154] , [155] , [156] , [157] , [158] , [159] , [160] , [161] ], messenger RNA (mRNA) [ [162] , [163] , [164] , [165] , [166] ], microRNA (miRNA) [ 167 ], protein mass spectrometry for seminal fluid detection [ 168 ], and microbiome analysis [ 169 , 170 ]. Although many new techniques are being described in the scientific literature, traditional methods for semen identification are still widely used in regular forensic casework [ 171 ].…”

Section: Advancements In Current Practicesmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Section: Advancements In Current Practicesmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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“…The advantage of MPS is that, in addition to detecting mRNA markers, the nucleotide sequence of the RNA transcript is also achieved. The detection of coding region SNPs (cSNPs) within the mRNA transcript has been introduced as a promising approach to associate a body fluid with a donor [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Comparison of cSNP genotypes within a body fluid-specific transcript to the corresponding genomic cSNP genotypes in reference samples from donors, may be used to associate a donor with a given body fluid.…”

Section: Introductionmentioning

confidence: 99%

Body Fluid Identification in Samples Collected after Intimate and Social Contact: A Comparison of Two mRNA Profiling Methods and the Additional Information Gained by cSNP Genotypes

Johannessen

Hanson

Gill

et al. 2023

Genes

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The ability to associate a contributor with a specific body fluid in a crime stain can aid casework investigation. The detection of body fluids combined with DNA analyses may supply essential information, but as the two tests are independent, they may not be associated. Recently, the analysis of coding region SNPs (cSNPs) within the RNA transcript has been proven to be a promising method to face this challenge. In this study, we performed targeted RNA sequencing of 158 samples (boxershorts, fingernail swabs and penile swabs) collected from 12 couples at different time points post-intimate contact and after non-intimate contact, using the Ion S5™ System and BFID-cSNP-6F assay. The aim of the study was to compare the performance of the MPS and CE methods in the detection of mRNA markers, and to associate body fluids with contributors by their cSNP genotypes. The results of the study show a lower success rate in the detection of vaginal mucosa by the MPS compared to the CE method. However, the additional information obtained with the cSNP genotypes could successfully associate body fluids with contributors in most cases.

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mRNA for Body Fluid and Individual Identification

Liu,

Wang,

et al. 2024

Electrophoresis

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Biological stains are one of the most important pieces of evidence, playing a multifaceted role in forensic investigations. An integral facet of forensic practice involves the identification of body fluids, typically achieved through chemical and enzymatic reactions. In recent decades, the introduction of mRNA markers has been posited as a pivotal advancement to augment the capabilities of body fluid identification (BFID). The mRNA coding region single‐nucleotide polymorphisms (cSNPs) also present notable advantages, particularly in the task of individual identification. Here, we review the specificity and stability of mRNA markers in the context of BFID and the prowess of mRNA polymorphism in individual identification. Additionally, innovative methods for mRNA detection are discussed.

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Identification of coding region SNPs from specific and sensitive mRNA biomarkers for the deconvolution of the semen donor in a body fluid mixture

Cited by 15 publications

References 44 publications

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Body Fluid Identification in Samples Collected after Intimate and Social Contact: A Comparison of Two mRNA Profiling Methods and the Additional Information Gained by cSNP Genotypes

mRNA for Body Fluid and Individual Identification

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