Identification of Comamonas testosteroni as an androgen degrader in sewage

Chen, Yi Lung; Wang, Chia Hsiang; Yang, Fu Chun; Ismail, Wael; Wang, Po Hsiang; Shih, Chao Jen; Wu, Yajing; Chiang, Yin-Ru

doi:10.1038/srep35386

Cited by 35 publications

(19 citation statements)

References 61 publications

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“…testosteroni [49][50][51], S. xenophaga [52,53] and N. oligotropha [54,55] were identified. These bacteria would be the indigenous bacteria in irrigation water and then colonized in the nutrient solution, and would aid in the self-purification of the recirculating nutrient solution.…”

Section: Discussionmentioning

confidence: 99%

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

Chen¹,

Li²,

Wang³

et al. 2019

Preprint

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Background: The ebb-and-flow system has ability to recirculate water and nutrients, and offers a good method to control nutrient leaching from greenhouses into the environment. However, the potential for the rapid spread of bacterial and fungal pathogens is the main hindrance for its adoption in vegetable seedlings production. Natural microflora has often shown a certain ability to suppress diseases.Results: Here, through 16S rRNA- and ITS1-targeted Illumina sequencing, the dynamic changes in bacterial and fungal communities in the recirculating nutrient solution were characterized for tomato plug seedlings cultivated in an ebb-and-flow system in summer and winter. Both bacterial number and microbial diversity in the nutrient solution increased with recirculating irrigation, and these changes differed between summer and winter. Pseudomonas was among the most predominant bacterial genera in the nutrient solution; its relative abundance gradually increased with recycling in summer but decreased dramatically in winter. In summer, the predominant bacteria also included Comamonas and Sediminibacterium , whose relative abundances in the nutrient solution decreased and increased, respectively. In winter, the nutrient solution was also colonized by Sphingomonas , predominantly at the late stage of irrigation. Among fungi, Amanita and Trichoderma were predominant in both summer and winter. Amanita gradually increased in summer but decreased rapidly after the first irrigation cycle in winter, whereas Trichoderma accumulated rapidly at the late stage of irrigation in both summer and winter. Alternaria and Fusarium were predominant in the nutrient solution only in summer, with the former decreased and the latter increased with recirculating irrigation. Moreover, Pseudomonas mediterranea , a potentially pathogenic bacterium, and Fusarium oxysporum , a fungal pathogen were present predominantly in winter and summer, respectively. Some potentially beneficial microbes functioning in plant-growth promotion and water self-purification, such as Sphingobium xenophagum , Trichoderma harzianum , and T. virens , were identified to be increased in the recirculating nutrient solution.Conclusions: The present data elucidate the bacterial and fungal dynamics in an ebb-and-flow system and provide useful information for pathogen control during tomato seedling production.

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Section: Discussionmentioning

confidence: 99%

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

Chen¹,

Li²,

Wang³

et al. 2019

Preprint

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“…One of the most commonly identified androgens in the environment is testosterone, its presence in the environment may cause a high percentage of males in the aquatic environment, appearance of male secondary sex characteristics in female fish, inhibition of vitellogenin induction, reduction of reproductive capacity and masculinization in mammals [ 26 ]. For example, defeminisation has been observed in fathead minnows that have been exposed to wastewater from cattle farms [ 27 ]. Moreover, it has been showed that male Atlantic salmon exhibit an odorant response to testosterone at extremely low concentration (0.003 ng/L) [ 28 ].…”

Section: Endocrine Disrupting Compounds (Edcs) In the Environment mentioning

confidence: 99%

“…As a result of the B ring cleavage and aromatization of the A ring of this compound, a secosteroid (3-hydroxy-9,10-seco-androsta-1,3,5(10)-triene-9,17-dione) is formed. Further degradation steps include hydroxylation of the A ring in position 4 and its cleavage by 3,4-dioxygenase to 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DHSA) [ 27 ]. These compounds may undergo decomposition to…”

Section: Microbiological Degradation Of Edcsmentioning

confidence: 99%

Suitability of Immobilized Systems for Microbiological Degradation of Endocrine Disrupting Compounds

Wojcieszyńska

Marchlewicz

2020

Molecules

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The rising pollution of the environment with endocrine disrupting compounds has increased interest in searching for new, effective bioremediation methods. Particular attention is paid to the search for microorganisms with high degradation potential and the possibility of their use in the degradation of endocrine disrupting compounds. Increasingly, immobilized microorganisms or enzymes are used in biodegradation systems. This review presents the main sources of endocrine disrupting compounds and identifies the risks associated with their presence in the environment. The main pathways of degradation of these compounds by microorganisms are also presented. The last part is devoted to an overview of the immobilization methods used for the purposes of enabling the use of biocatalysts in environmental bioremediation.

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“…Comamonas is a genus of aerobic proteobacteria, generally considered as environmental bacteria with less pathogenicity. It is reported that one of the Comamonas species, C. testosterone, aerobically degrades testosterone when cultured in testosteronecontaining media [67]. In the STN, the enrichment of Comamonas in sewage suggests the possibility of its role in decontamination of medical compounds.…”

Section: Metagenomic Analysis Of Tank Ora In Each Buildingmentioning

confidence: 99%

Metagenomic survey and whole genome sequencing of antimicrobial resistant bacteria in the sewage of a Japanese hospital

Katagiri

Kuroda

Sekizuka

et al. 2020

Preprint

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Background The dissemination of antimicrobial-resistant bacteria (ARB) and the transfer of antimicrobial resistance genes (ARGs) are a threat to public health. Antibiotics are indispensable therapeutic agents essential for the treatment of infectious diseases; however, inappropriate use of antibiotics leads to the emergence of ARB. It is established that hospitals are closely involved in the spread of antimicrobial resistance (AMR), which impedes antibiotic treatment and subsequently increases mortality. In addition, excrement of patients or healthy carriers of ARB are discharged from the hospital sewage through the wastewater treatment plant (WWTP) into the rivers, causing an AMR burden on the environment.Method Metagenomic analysis was performed on the hospital sewage samples, followed by whole genome sequencing of the extended spectrum β-lactamase (ESBL)-producing organisms (EPOs). A comparative genome analysis was also performed between EPO isolates from sewage and clinical isolates.Results Metagenomic analysis showed that the hospital sewage tanks had bacterial flora corresponding to the human gut. During the study period, the hospital was relocated to a newly constructed building with new sewage tanks; however, the presence of ARB/ARGs in the new hospital sewage tanks became markedly equivalent to that of the old hospital within one month. The ESBL blaCTX−M and carbapenemase blaIMP genes were not much detected in the original hospital sewage samples by metagenome analysis, but selection on CHROMagar ESBL increased the sensitivity to detect those β-lactamase genes. Comparative genome analysis between sewage and clinical EPO isolates revealed partial similarity; however, most EPO isolates exhibited a notable difference (≥ 50) in single nucleotide variations based on core-genome phylogeny. This result suggests that only some of the sewage EPO isolates were originated from the clinical patient. Therapeutic agents in the hospital sewage were analyzed and the concentration of levofloxacin and clarithromycin was 0.0325 and 0.0135 µg/mL, respectively.Conclusions Whole genome analysis between sewage and clinical isolates suggested that healthy or asymptomatic carriers may be involved in the contamination of hospital sewage. Moreover, the hospital sewage tank may serve as a hotspot for the horizontal transfer of ARGs under the selective pressure of antimicrobial agents. Therefore, ARB monitoring in hospital sewage is expected to detect the presence of carriers prior to nosocomial ARB outbreaks. In addition, hospital wastewater should be treated suitably to bring ARB below detectable levels to reduce the environmental AMR burden.

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Identification of Comamonas testosteroni as an androgen degrader in sewage

Cited by 35 publications

References 61 publications

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

Suitability of Immobilized Systems for Microbiological Degradation of Endocrine Disrupting Compounds

Metagenomic survey and whole genome sequencing of antimicrobial resistant bacteria in the sewage of a Japanese hospital

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