Identification of conserved peptides containing B‐cell epitopes of
            <i>Babesia bovis</i>
            <scp>AMA</scp>
            ‐1 and their potential as diagnostics candidates

Barreda, Dante; Hidalgo-Ruiz, Mario; Hernández-Ortíz, Rubén; Ramos, Jonathan Hernández; Galindo-Velasco, Edelmira; Mosqueda, Juan

doi:10.1111/tbed.13213

Cited by 7 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the fact that the immune system recognizes conserved B-epitopes and generates antibodies against them in most cattle in the endemic areas tested supports the hypothesis that these conserved B-cell epitopes are immunogenic. These results are similar to those observed in other studies analyzing conserved B-cell epitope immunogenicity in other membrane-bound or organelle-secreted antigens in both B. bigemina and B. bovis [ 14 , 27 , 28 , 35 , 36 , 37 ]. This similarity suggests that those antigens are exposed to the immune system.…”

Section: Discussionsupporting

confidence: 91%

Spherical Body Protein 4 from Babesia bigemina: A Novel Gene That Contains Conserved B-Cell Epitopes and Induces Cross-Reactive Neutralizing Antibodies in Babesia ovata

et al. 2023

Self Cite

View full text Add to dashboard Cite

Bovine babesiosis is a tick-transmitted disease caused by intraerythrocytic protozoan parasites of the genus Babesia. Its main causative agents in the Americas are Babesia bigemina and Babesia bovis, while Babesia ovata affects cattle in Asia. All Babesia species secrete proteins stored in organelles of the apical complex, which are involved in all steps of the invasion process of vertebrate host cells. Unlike other apicomplexans, which have dense granules, babesia parasites instead have large, round intracellular organelles called spherical bodies. Evidence suggests that proteins from these organelles are released during the process of invading red blood cells, where spherical body proteins (SBPs) play an important role in cytoskeleton reorganization. In this study, we characterized the gene that encodes SBP4 in B. bigemina. This gene is transcribed and expressed in the erythrocytic stages of B. bigemina. The sbp4 gene consists of 834 nucleotides without introns that encode a protein of 277 amino acids. In silico analysis predicted a signal peptide that is cleaved at residue 20, producing a 28.88-kDa protein. The presence of a signal peptide and the absence of transmembrane domains suggest that this protein is secreted. Importantly, when cattle were immunized with recombinant B. bigemina SBP4, antibodies identified B. bigemina and B. ovata merozoites according to confocal microscopy observations and were able to neutralize parasite multiplication in vitro for both species. Four peptides with predicted B-cell epitopes were identified to be conserved in 17 different isolates from six countries. Compared with the pre-immunization sera, antibodies against these conserved peptides reduced parasite invasion in vitro by 57%, 44%, 42%, and 38% for peptides 1, 2, 3, and 4, respectively (p < 0.05). Moreover, sera from cattle infected with B. bigemina cattle contained antibodies that recognized the individual peptides. All these results support the concept of spb4 as a new gene in B. bigemina that should be considered a candidate for a vaccine to control bovine babesiosis.

show abstract

Section: Discussionsupporting

confidence: 91%

Spherical Body Protein 4 from Babesia bigemina: A Novel Gene That Contains Conserved B-Cell Epitopes and Induces Cross-Reactive Neutralizing Antibodies in Babesia ovata

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…De acuerdo a los resultados obtenidos, posiblemente la región caracterizada en esta investigación podría generar una respuesta inmune eficaz debido al elevado grado de conservación que presenta, sugiriendo ser un candidato potencial si se utiliza como vacuna contra garrapatas R. microplus en diferentes regiones geográficas. Recientemente, se han realizado trabajos utilizando genes de vital importancia para Babesia spp., demostrando que la selección bioinformática de regiones hidrofílicas, inmunogénicas y conservadas permiten identificar péptidos con elevado potencial para el desarrollo de vacunas (Hernández-Silva et al, 2018;Mosqueda et al, 2019;Barreda et al, 2020). Por otro lado, existen investigaciones realizadas previamente donde se ha evidenciado que la inmunización de péptidos derivados del antígeno Bm86 son capaces de generar una respuesta inmune protectora, que se refleja en un mejor reconocimiento por los anticuerpos anti-Bm86 en el sitio blanco (intestino medio) de la garrapata R. microplus (Patarroyo et al, 2002(Patarroyo et al, , 2009.…”

Section: Discussionunclassified

Characterization of an immunogenic region of the Bm86 antigen as an improved vaccine against the Rhipicephalus microplus tick

Valdez-Espinoza¹,

Gómez‐Romero²,

Hernández-Ortíz³

et al. 2021

Mexjbiotechnol

View full text Add to dashboard Cite

One of the main health problems in cattle farming is infestations caused by ticks. The most important species is Rhipicephalus microplus, which affects 80% of bovine population worldwide. Its control is mainly based on the use of ixodicides. However, excessive and inappropriate use has generated multi-resistant strains of ticks. Alternatively, immune control has been proposed as an important method to reduce tick populations. The recombinant Bm86 vaccine is the only commercially available and reports variations in its efficacy due to polymorphisms in the gene's coding sequence. Therefore, this work aimed to characterize an immunogenic region of Bm86 strain R. microplus "Media Joya" to propose a vaccine candidate that is conserved and with minimal variability in its efficacy. Computational tools, molecular biology techniques, and phylogenetic analysis were used. As a result, an immunogenic region consisting of 178 amino acids and 98-100% identity/similarity were obtained concerning strains of R. microplus ticks from the Americas. Furthermore, is necessary to evaluate the immunoprotective potential of the Bm86 region characterized in this work as an anti-tick vaccine.

show abstract

“…Nevertheless, reactivity against serum from animals in different regions of the country was noted. Modifications such as the use of several peptides from one or more proteins or changing the format of ELISA assays were suggested to upgrade the diagnosis system [ 50 ].…”

Section: Peptides In Diagnosismentioning

confidence: 99%

The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review

Montes-de-Oca-Jiménez²,

Vázquez-Chagoyán³

et al. 2022

Veterinary Sciences

View full text Add to dashboard Cite

Peptides constitute an alternative and interesting option to develop treatments, vaccines, and diagnostic tools as they demonstrate their scope in several health aspects; as proof of this, commercial peptides for humans and animals are available on the market and used daily. This review aimed to know the role of peptides in the field of veterinary diagnosis, and include peptide-based enzyme-linked immunosorbent assay (pELISA), lateral flow devices, and peptide latex agglutination tests that have been developed to detect several pathogens including viruses and bacteria of health and production relevance in domestic animals. Studies in cattle, small ruminants, dogs, cats, poultry, horses, and even aquatic organisms were reviewed. Different studies showed good levels of sensitivity and specificity against their target, moreover, comparisons with commercial kits and official tests were performed which allowed appraising their performance. Chemical synthesis, recombinant DNA technology, and enzymatic synthesis were reviewed as well as their advantages and drawbacks. In addition, we discussed the intrinsic limitations such as the small size or affinity to polystyrene membrane and mention several strategies to overcome these problems. The use of peptides will increase in the coming years and their utility for diagnostic purposes in animals must be evaluated.

show abstract

Identification of conserved peptides containing B‐cell epitopes of Babesia bovis AMA ‐1 and their potential as diagnostics candidates

Cited by 7 publications

References 39 publications

Spherical Body Protein 4 from Babesia bigemina: A Novel Gene That Contains Conserved B-Cell Epitopes and Induces Cross-Reactive Neutralizing Antibodies in Babesia ovata

Spherical Body Protein 4 from Babesia bigemina: A Novel Gene That Contains Conserved B-Cell Epitopes and Induces Cross-Reactive Neutralizing Antibodies in Babesia ovata

Characterization of an immunogenic region of the Bm86 antigen as an improved vaccine against the Rhipicephalus microplus tick

The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review

Contact Info

Product

Resources

About