Identification of eae genes in <i>Escherichia coli</i> strains isolated from pigs with postweaning diarrhoea

Osek, Jacek

doi:10.1136/vr.148.8.241

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…In the present study, we found only one O45:H- isolate (0.9%) positive for the eae gene. In accordance with our results, Frydendahl [ 7 ] and Osek [ 26 ] also found only 1% (3 of 219) and 3% (6 of 224) of Danish and Polish PWD isolates carrying the eae gene, respectively. F17-producing E. coli are commonly isolated from calves with or without diarrhoea [ 27 ].…”

Section: Discussionsupporting

confidence: 92%

Serotypes, virulence genes, and PFGE profiles of Escherichia coliisolated from pigs with postweaning diarrhoea in Slovakia

et al. 2006

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BackgroundPostweaning diarrhoea (PWD) in pigs is usually the main infectious problem of large-scale farms and is responsible for significant losses worldwide. The disease is caused mainly by enterotoxigenic E. coli (ETEC) and Shiga-toxin producing E. coli (STEC). In this study a total of 101 E. coli isolated from pigs with PWD in Slovakia were characterized using phenotypic and genotypic methods.ResultsThese 101 isolates belonged to 40 O:H serotypes. However, 57% of the isolates belonged to only six serotypes (O9:H51, O147:H-, O149:H10, O163:H-, ONT:H-, and ONT:H4), including two new serotypes (O163:H- and ONT:H4) not previously found among porcine ETEC and STEC isolated in other countries. Genes for EAST1, STb, STa, LT and Stx2e toxins were identified in 64%, 46%, 26%, 20%, and 5% of isolates, respectively. PCR showed that 35% of isolates carried genes for F18 colonization factor, and further analyzed by restriction endonuclease revealed that all of them were F18ac. Genes for F4 (K88), F6 (P987), F17, F5 (K99), F41, and intimin (eae gene) adhesins were detected in 19 %, 5%, 3%, 0.9%, 0.9%, and 0.9% of the isolates, respectively. The study of genetic diversity, carried out by PFGE of 46 representative ETEC and STEC isolates, revealed 36 distinct restriction profiles clustered in eight groups. Isolates of the same serotype were placed together in the dendrogram, but high degree of polymorphism among certain serotypes was detected.ConclusionSeropathotype O149:H10 LT/STb/EAST1/F4 (14 isolates) was the most commonly detected followed by O163:H- EAST1/F18 (six isolates), and ONT:H4 STa/STb/Stx2e/F18 (five isolates). Interestingly, this study shows that two new serotypes (O163:H- and ONT:H4) have emerged as pig pathogens in Slovakia. Furthermore, our results show that there is a high genetic variation mainly among ETEC of O149:H10 serotype.

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Section: Discussionsupporting

confidence: 92%

Serotypes, virulence genes, and PFGE profiles of Escherichia coliisolated from pigs with postweaning diarrhoea in Slovakia

et al. 2006

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“…On the other hand, four stx 2‐positive isolates did not possess the eaeA virulence trait. The prevalence of the intimin marker among non‐bovine E coli strains, including strains isolated from pigs, has been reported (Zhu and others 1994, Holland and others 2000, Kim and others 2001, Osek 2001), but the y intimin variant identified in the present study was not described. The eae‐γ subtype has previously been found among several O157:H7 – STEC isolated from cattle and from people with haemolytic uraemic syndrome in many countries (Adu‐Bobie and others 1998, Batchelor and others 1999, Schmidt and others 1999, Oswald and others 2000).…”

Section: Discussioncontrasting

confidence: 53%

Identification of Escherichia coli 01 57:H7‐ strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR

Osek

2002

Veterinary Record

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Escherichia coli isolated from pigs with postweaning diarrhoea were examined by PCR for the presence of the O157 rfb gene responsible for the biosynthesis of E coli O157 lipopolysaccharide. Among the 372 isolates tested, 38 (10.2 per cent) were of the O157 serogroup, but none of these possessed the H7 determinant. Further analysis of the E coli O157 isolates revealed that seven of them had the genes responsible for the production of Shiga toxin 1 and eaeA intimin, four other strains had genes responsible for the production of Shiga toxin 2, and four other strains were positive for the enterohaemolysin gene.

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“…Further studies have proven that the eae gene of porcine EPEC prototype E. coli 1930 (O45) strain was a member of the beta intimin group and showed the highest similarity with the rabbit EPEC strains . Such strains may be present in small numbers in the pig population not only in North America but also in Europe as well (Osek, 2001). However, the overall significance of porcine EPEC strains cannot be judged on the basis of the available data.…”

Section: Epec (And Ehec) In Pigs and Calvesmentioning

confidence: 99%

Preface

Holzapfel¹,

Naughton²

2005

Microbial Ecology in Growing Animals

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Enteric diseases of pigs and calves owing to Escherichia coli typically appear during the first few days (and weeks) of life. The so far recognized pathotypes of E. coli involved are the enterotoxic E. coli (ETEC), verotoxic E. coli (VTEC), enteropathogenic E. coli (EPEC), and necrotoxic E. coli (NTEC). The first step in the pathogenesis of all these types is to adhere to the intestinal microvilli with or without inducing morphological lesions and produce specific toxins acting locally on enterocytes and/or absorbed into the bloodstream. This action is assured by specific ligand (adhesins) and receptor interactions which are characteristic of the pathotypes involved and may vary according to the animal species. The host-adapted adhesin/receptor systems are targets of several preventive measures including vaccines, receptor blocking and breeding for genetic resistance. They also provide the basis for cross-species infections including zoonoses. Therefore they deserve the attention of epidemiologists, research scientists and technologists. Besides, they provide tools for increased understanding of molecular pathogenesis, and offer excellent models for comparative studies of different disease entities of enteric colibacillosis in humans.

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Identification of eae genes in Escherichia coli strains isolated from pigs with postweaning diarrhoea

Abstract: Ovine infectious keratoconjunctivitis: a microbiological study of clinically unaffected and affected sheep's eyes with special reference to Mycoplasma conjunctivae. Veterinary Record 125, 253-256

Cited by 5 publications

References 18 publications

Serotypes, virulence genes, and PFGE profiles of Escherichia coliisolated from pigs with postweaning diarrhoea in Slovakia

Serotypes, virulence genes, and PFGE profiles of Escherichia coliisolated from pigs with postweaning diarrhoea in Slovakia

Identification of Escherichia coli 01 57:H7‐ strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR

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