Identification of embryonic stem cell activities in an embryonic cell line derived from marine medaka (Oryzias dancena)

Abstract

This study was conducted to identify embryonic stem cell (ESC) activities of a long-term cultured embryonic cell line previously derived from blastula-stage Oryzias dancena embryos. Five sub-cell lines were established from the embryonic cell line via clonal expansion of single cells. ESC activities, including clonogenicity, alkaline phosphatase (AP) activity, and differentiation capacity, were examined in the five sub-cell lines. We observed both clonogenicity and AP activity in all five sub-cell lines, but the proportion of cells that exhibited both properties was significantly different among them. Even though we detected different formation rates and sizes of embryoid body (EB) among these cells, all lines were stably able to form EBs and further induction for differentiation showed their capability to differentiate into other cell types in a spontaneous manner. From this study, we determined that the embryonic cell lines examined possessed heterogeneous ESC activities and can be utilized as a marine model system for fish ESC-based research.

Toxicity assessment of pollutants sorbed on environmental sample microplastics collected on beaches: Part I-adverse effects on fish cell line

“…The North Pacific has experienced a dramatic increase in meso and macro-plastics since the 1990s (Barnes et al, 2009). Particularly high levels of microplastic contamination were recorded on sandy beaches in Korea (Lee et al, 2015). Law pieces/km -2 .…”

Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

“…Significant differences in cell growth were observed between the cells cultured in L15 and DMEM. On the basis of many previous studies that reported successful fish cell culture in an air atmosphere (Abdul Majeed et al, 2013;Kim et al, 2014;Lee et al, 2015), we used an air atmosphere instead of CO 2 gas to culture S. schlegeli ovary-dissociated cells. L15 medium was originally designed for use in culture without CO 2 , and DMEM was supplemented with HEPES to control the physiological pH .…”

Effects of the Developmental Stage of Extract Donor Embryos on the Culture of Marine Medaka Oryzias dancena Embryonic Stem Cell-like Cells

“…300 (ODESCLC1; Lee et al, 2015), AP 염색 AP , (Thomson et al, 1995;Pera et al, 2000;Ivanova et al, 2006), AP Béjar et al, 2002;Chen et al, 2003a Extracts from 100 embryos of each developmental stage were used for measurement of protein concentration. Significant high protein concentration was detected in the extracts from stage 9 embryos (P<0.0001) and the concentration decreased as development is progressed.…”

“…( Lee et al, 2015). In this case, the effects of two developmental stages, 9 and 28 stages, of extract donor embryos were investigated on the growth, colony forming ability, alkaline phosphatase (AP) activity, and embryoid body (EB) formation of ESC-like cells.…”