Identification of estrogen-responsive genes in the testis of sea bream (Sparus auratus) using suppression subtractive hybridization

Pinto, Patrícia I. S.; Teodósio, Rita; Galay-Burgos, Malyka; Power, Deborah M.; Sweeney, Glen E.; Canário, Adelino V. M.

doi:10.1002/mrd.20402

Cited by 45 publications

(23 citation statements)

References 62 publications

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“…Although we classified the biological process of transferrin as transport, a glycoprotein responsible for the transport and delivery of iron to cells, it is also related to the innate immune response of fish because of the importance of free iron for bacterial infections (Stafford and Belosevic, 2003;Pardo et al, 2008). The transferrin gene, together with other genes that we found in liver such as apolipoprotein and fibrinogen, has been shown to be regulated by estrogens in females (Pinto et al, 2006). In our analysis we found the transferrin transcript only in females whereas apolipoprotein and fibrinogen transcripts were detected in both sexes.…”

Section: Discussionmentioning

confidence: 99%

Comparative expression analysis in mature gonads, liver and brain of turbot (Scophthalmus maximus) by cDNA-AFLPS

Taboada

Robledo

Palacio

et al. 2012

Gene

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Turbot is one of the most important farmed fish in Europe. This species exhibits a considerable sexual dimorphism in growth and sexual maturity that makes the all-female production recommended for turbot farming. Our knowledge about the genetic basis of sex determination and the molecular regulation of gonad differentiation in this species is still limited. Our goal was to identify and compare gene expression and functions between testes and ovaries in adults in order to ascertain the relationship between the genes that could be involved in the gonad differentiation or related to the sex determination system. The identification of differentially expressed sex related genes is an initial step towards understanding the molecular mechanisms of gonad differentiation. For this, we carried out a transcriptome analysis based on cDNA-AFLP technique which allowed us to obtain an initial frame on sex-specific gene expression that will facilitate further analysis especially along the critical gonad differentiating period. With the aim of widening the study on sex-biased gene expression we reproduced the same experiments in two somatic tissues: liver and brain. We have selected the liver because it is the most analyzed one regarding sexual dimorphic gene expression and due to its importance in steroid hormones metabolism and brain because the functional relationship between brain and gonad is documented. We found slight but important differences between sexes which deserve further investigation.

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Section: Discussionmentioning

confidence: 99%

Comparative expression analysis in mature gonads, liver and brain of turbot (Scophthalmus maximus) by cDNA-AFLPS

Taboada

Robledo

Palacio

et al. 2012

Gene

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“…Fish used for the present coldchallenge experiment were age matched (7 months post-hatch). Potential sex-related differences were not expected because the sea bream is a hermaphrodite and during the first year mature as males (Pinto et al, 2006;Zohar et al, 1978). However, significant differences in mass and length existed between fish from the different thermal regimes (P<0.001; Table 1).…”

Section: Materials and Methods Early Life Programmingmentioning

confidence: 99%

Thermal imprinting modifies bone homeostasis in cold challenged sea bream (Sparus aurata, L.)

Mateus

Costa

Gisbert

et al. 2017

Journal of Experimental Biology

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Fish are ectotherms and temperature plays a determinant role in their physiology, biology and ecology, and is a driver of seasonal responses. The present study assessed how thermal imprinting during embryonic and larval stages modified the response of adult fish to low water temperature. We targeted the gilthead sea bream, which develops a condition known as winter syndrome when it is exposed to low water temperatures. Eggs and larvae of sea bream were exposed to four different thermal regimes and then the response of the resulting adults to a low temperature challenge was assessed. Sea bream exposed to a high-low thermal regime as eggs and larvae (HLT; 22°C until hatch and then 18°C until larvae-juvenile transition) had increased plasma cortisol and lower sodium and potassium in response to a cold challenge compared with the other thermal history groups. Plasma glucose and osmolality were increased in coldchallenged HLT fish relative to the unchallenged HLT fish. Cold challenge modified bone homeostasis/responsiveness in the lowhigh thermal regime group (LHT) relative to other groups, and ocn, ogn1/2, igf1, gr and trα/β transcripts were all downregulated. In the low temperature group (LT) and HLT group challenged with a low temperature, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) activities were decreased relative to unchallenged groups, and bone calcium content also decreased in the LT group. Overall, the results indicate that thermal imprinting during early development of sea bream causes a change in the physiological response of adults to a cold challenge.

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“…Protein profiling revealed lower levels for apoA-I and apoA-IV precursor proteins in the liver, but the picture for the other apolipoproteins is not clear and additional studies are required to confirm this trend, especially in view of controversial and incomplete studies reported in the literature (see below) for fish. Due to technical (60) and in the liver of zebrafish (28) and of the common carp (55) after 17␣-ethynylestradiol (EE 2 ) treatment. …”

Section: Inverse Relationship Between Vtgs and Apolipoproteinsmentioning

confidence: 99%

Insight into molecular pathways of retinal metabolism, associated with vitellogenesis in zebrafish

Levi

Ziv

Admon

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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Levi L, Ziv T, Admon A, Levavi-Sivan B, Lubzens E. Insight into molecular pathways of retinal metabolism, associated with vitellogenesis in zebrafish. Am J Physiol Endocrinol Metab 302: E626 -E644, 2012. First published December 28, 2011; doi:10.1152/ajpendo.00310.2011Retinal is the main retinoid stored in oviparous eggs of fish, amphibians, and reptiles, reaching the oocytes in association with vitellogenins, the yolk precursor proteins. During early presegmentation stages of zebrafish embryos, retinal is metabolized to retinoic acid (RA), which regulates genes involved in cell proliferation, differentiation, and tissue function and is therefore essential for normal embryonic development. While synthesis of vitellogenin and its regulation by 17␤-estradiol (E2) were extensively investigated, pathways for retinal synthesis remain obscure. We determined the expression pattern of 46 candidate genes, aiming at identifying enzymes associated with retinal synthesis, ascertaining whether they were regulated by E2, and finding pathways that could fulfill the demand for retinoids during vitellogenesis. Genes associated with retinal synthesis were upregulated in liver (rdh10, rdh13, sdr) and surprisingly also in intestine (rdh13) and ovary (rdh1, sdr), concomitantly with higher gene expression and synthesis of vitellogenins in liver but also in extrahepatic tissues, shown here for the first time. Vitellogenin synthesis in the ovary was regulated by E 2. Gene expression studies suggest that elevated retinal synthesis in liver, intestine, and ovary also depends on cleavage of carotenoids (by Bcdo2 or Bmco1), but in the ovary it may also be contingent on higher uptake of retinol from the circulatory system (via Stra6) and retinol synthesis from retinyl esters (by Lpl). Decrease in oxidation (by Raldh2 or Raldh3) of retinal to RA and/or degradation of RA (by Cyp26a1) may also facilitate higher hepatic retinal levels. Together, these processes enable meeting the putative demands of retinal for binding to vitellogenins. Bioinformatic tools reveal multiple hormone response elements in the studied genes, suggesting complex and intricate regulation of these processes. extrahepatic vitellogenin synthesis; regulatory response elements; retinoids; carotenoids RETINAL IS THE PREDOMINANT RETINOID in eggs and oocytes of marine fish and some freshwater fish species and may constitute almost the entire pool of retinoids in these eggs. In oviparous eggs of nonmammalian vertebrates such as teleosts, amphibians, and birds, maternally derived retinal is bound via a Schiff-base linkage to vitellogenins (Vtgs) (15, 32, 62, 63, 64, 72 reviewed in 50). The retinal in fish oocytes and eggs consists of several isomers, whereas the all-trans form is the most abundant in zebrafish embryos (15,44). The use of the all-trans isomer of retinal for major retinoid storage is unique to reptiles, anamniotic vertebrates (teleosts and amphibians), and eggs of ascidian (33,34). This is in contrast to higher vertebrates (birds and mammals), where retinol is the...

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Identification of estrogen-responsive genes in the testis of sea bream (Sparus auratus) using suppression subtractive hybridization

Cited by 45 publications

References 62 publications

Comparative expression analysis in mature gonads, liver and brain of turbot (Scophthalmus maximus) by cDNA-AFLPS

Comparative expression analysis in mature gonads, liver and brain of turbot (Scophthalmus maximus) by cDNA-AFLPS

Thermal imprinting modifies bone homeostasis in cold challenged sea bream (Sparus aurata, L.)

Insight into molecular pathways of retinal metabolism, associated with vitellogenesis in zebrafish

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