Identification of exon 1 of prostase/KLK-L1 gene

Obiezu, Christina V.; Yousef, George M.; Diamandis, Eleftherios P.

doi:10.1016/s0009-9120(00)00060-6

Cited by 5 publications

(8 citation statements)

References 3 publications

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“…1A) was used to amplify all KLK4 transcripts (Fig. 1A) (Obiezu & Diamandis 2000, Korkmaz et al 2001, Myers & Clements 2001. While the KLK4-254 and exon 1 deleted KLK4-205 transcripts would give the same PCR product of 526 bp (Obiezu & Diamandis 2000, Korkmaz et al 2001, Myers & Clements 2001, four other transcripts could potentially be amplified.…”

Section: Resultsmentioning

confidence: 99%

“…While the KLK4-254 and exon 1 deleted KLK4-205 transcripts would give the same PCR product of 526 bp (Obiezu & Diamandis 2000, Korkmaz et al 2001, Myers & Clements 2001, four other transcripts could potentially be amplified. These are exon 1 deleted versions of KLK4-195 and KLK4-159 (609 and 389 bp) with an intron III insertion or exon 4 deletion that would encode hK4 proteins of 146 and 110 aa respectively, and thus are designated KLK4-146 and KLK4-110 (Obiezu & Diamandis 2000, Korkmaz et al 2001, Myers & Clements 2001. KLK4-75 and its exon 4 deleted forms would give PCR products of 538 and 401 bp respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The kallikrein 4 gene (KLK4) consists of five exons, and several alternative KLK4 transcripts (reviewed by Kurlender et al 2005) have been reported (Obiezu & Diamandis 2000, Korkmaz et al 2001, Myers & Clements 2001. These include fulllength KLK4 (KLK4-254), exon 1 deleted forms that encode proteins missing the signal peptide and proregion of the zymogen, and several transcripts with splice variations between exons 2 and 5.…”

Section: Introductionmentioning

confidence: 99%

“…These include fulllength KLK4 (KLK4-254), exon 1 deleted forms that encode proteins missing the signal peptide and proregion of the zymogen, and several transcripts with splice variations between exons 2 and 5. The latter exhibit a frame shift in the coding region and generate premature stop codons giving rise to truncated protein products that do not have the essential serine and/or aspartic acid residues of the catalytic triad (Obiezu & Diamandis 2000, Korkmaz et al 2001, Myers & Clements 2001. However, two KLK4 transcripts, the full-length KLK4-254 and one of the exon 1 deleted transcripts (KLK4-205), encode potentially active serine proteases.…”

Section: Introductionmentioning

confidence: 99%

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Compartmentalized expression of kallikrein 4 (KLK4/hK4) isoforms in prostate cancer: nuclear, cytoplasmic and secreted forms

Dong

Bui²,

Odorico³

et al. 2005

Endocr Relat Cancer

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The prostate-specific antigen-related serine protease gene, kallikrein 4 (KLK4), is expressed in the prostate and, more importantly, overexpressed in prostate cancer. Several KLK4 mRNA splice variants have been reported, but it is still not clear which of these is most relevant to prostate cancer. Here we report that, in addition to the full-length KLK4 (KLK4-254) transcript, the exon 1 deleted KLK4 transcripts, in particular, the 5 0 -truncated KLK4-205 transcript, is expressed in prostate cancer. Using V5/His6 and green fluorescent protein (GFP) carboxy terminal tagged expression constructs and immunocytochemical approaches, we found that hK4-254 is cytoplasmically localized, while the N-terminal truncated hK4-205 is in the nucleus of transfected PC-3 prostate cancer cells. At the protein level, using anti-hK4 peptide antibodies specific to different regions of hK4-254 (N-terminal and C-terminal), we also demonstrated that endogenous hK4-254 (detected with the N-terminal antibody) is more intensely stained in malignant cells than in benign prostate cells, and is secreted into seminal fluid. In contrast, for the endogenous nuclearlocalized N-terminal truncated hK4-205 form, there was less difference in staining intensity between benign and cancer glands. Thus, KLK4-254/hK4-254 may have utility as an immunohistochemical marker for prostate cancer. Our studies also indicate that the expression levels of the truncated KLK4 transcripts, but not KLK4-254, are regulated by androgens in LNCaP cells. Thus, these data demonstrate that there are two major isoforms of hK4 (KLK4-254/hK4-254 and KLK4-205/hK4-205) expressed in prostate cancer with different regulatory and expression profiles that imply both secreted and novel nuclear roles.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Compartmentalized expression of kallikrein 4 (KLK4/hK4) isoforms in prostate cancer: nuclear, cytoplasmic and secreted forms

Dong

Bui²,

Odorico³

et al. 2005

Endocr Relat Cancer

View full text Add to dashboard Cite

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“…KLK4, a more recently identified member of the KLK family, is a highly prostate-enriched gene that is also androgen regulated (13)(14)(15)(16)(17). The KLK4 gene encodes several splice isoforms (16,(18)(19)(20), and it is now clear that it gives rise to at least eight different mRNA forms through alternative splicing and/or alternative transcription start sites (21). These transcripts are expected to encode at least seven different protein moieties (21).…”

Section: Introductionmentioning

confidence: 99%