Identification of expressed sequence tags preferentially expressed in human placentas by in silico subtraction

Abstract

In silico subtraction identified 44 previously studied genes involved in placental physiology as well as 63 EST clusters preferentially expressed in placental tissue, which may serve as targets for future studies seeking novel markers for prenatal diagnosis or to better understand placental genetics.

Placental Regulation of Peptide Hormone and Growth Factor Activity by proMBP1

“…All tissues analyzed express PAPPA and proMBP [20]. The genes encoding PAPPA and proMBP are some of the most highly expressed in the human placenta [41], and the levels of circulating PAPPA increases during pregnancy, reaching approximately 25 mg/L at term [5]. PAPPA is implicated in placental growth and function, and low PAPPA levels in the circulation of pregnant women are associated with low birth weight [42], Down syndrome [43], Cornelia de Lange syndrome [44], and miscarriage [45].…”

In silico strategy for detection of target candidates for antibody therapy of solid tumors

“…Instead we used a collection of prior determined genes and transcripts as an entry point. Here we preferred the RefSeq database, over the frequently used UniGene database (Miner and Rajkovic, 2003;Pao et al, 2006). RefSeq offers a comprehensive non-redundant set of genomic, transcript and protein sequences.…”

“…The principle of using EST source distribution for in silico prediction of transcript tissue specificity, also referred to as 'electronic Northern', 'eNorthern', 'DigiNorthern' or 'in silico subtraction' is described as an efficient approach for the identification of tissue specific transcripts (Schmitt et al, 1999;Rajkovic et al, 2001;Katsanis et al, 2002;Miner and Rajkovic, 2003;Pao et al, 2006).…”

Census of orthologous genes and self-organizing maps of biologically relevant transcriptional patterns in chickens (Gallus gallus)

“…PEGs were likely to be critical to specialized functions in tissues/organs (Takeda et al, 1993;Liew et al, 1994;Miner and Rajkovic, 2003). For example, the bursa of Fabricius is a special gut-associated lymphoid tissue of the chicken located behind the cloaca, where chicken B cells create their immunoglobulin repertoire by gene conversion.…”