Identification of four Donax species by PCR–RFLP analysis of cytochrome c oxidase subunit I (COI)

Nantón, Ana; Freire, Ruth; Arias‐Pérez, Alberto; Gaspar, Miguel B.; Méndez, Josefina

doi:10.1007/s00217-015-2416-z

Cited by 11 publications

(13 citation statements)

References 24 publications

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“…2012 [ 18 ] and Nantón et al . 2015 [ 19 ] molecular protocols developed in our laboratory. Voucher specimens and their shells were deposited at the malacology collections of the Museo Nacional de Ciencias Naturales (MNCN), Madrid (Spain) ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

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First complete female mitochondrial genome in four bivalve species genus Donax and their phylogenetic relationships within the Veneroida order

et al. 2017

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BackgroundFour species of the genus Donax (D. semistriatus, D. trunculus, D. variegatus and D. vittatus) are common on Iberian Peninsula coasts. Nevertheless, despite their economic importance and overexploitation, scarce genetic resources are available. In this work, we newly determined the complete mitochondrial genomes of these four representatives of the family Donacidae, with the aim of contributing to unveil phylogenetic relationships within the Veneroida order, and of developing genetic markers being useful in wedge clam identification and authentication, and aquaculture stock management.Principal findingsThe complete female mitochondrial genomes of the four species vary in size from 17,044 to 17,365 bp, and encode 13 protein-coding genes (including the atp8 gene), 2 rRNAs and 22 tRNAs, all located on the same strand. A long non-coding region was identified in each of the four Donax species between cob and cox2 genes, presumably corresponding to the Control Region. The Bayesian and Maximum Likelihood phylogenetic analysis of the Veneroida order indicate that all four species of Donax form a single clade as a sister group of other bivalves within the Tellinoidea superfamily. However, although Tellinoidea is actually monophyletic, none of its families are monophyletic.ConclusionsSequencing of complete mitochondrial genomes provides highly valuable information to establish the phylogenetic relationships within the Veneroida order. Furthermore, we provide here significant genetic resources for further research and conservation of this commercially important fishing resource.

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Section: Methodsmentioning

confidence: 99%

“…trunculus in natural beds may contain other species of the genus with lesser economical value and may be marketed together. However, despite their overexploitation and economic importance, relatively few genetic resources are available for this species [ 15 , 17 ] and the whole genus [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

First complete female mitochondrial genome in four bivalve species genus Donax and their phylogenetic relationships within the Veneroida order

et al. 2017

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“…Until recently2526 and despite the overexploitation threat faced by this shellfish resource, D. trunculus has not been studied from a conservation biology or genetics perspective. The few studies that have paid any attention to its genetic makeup have focused on its genome structure27 or its mitochondrial DNA inheritance2528 only, but not with the aim to characterise its diversity.…”

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confidence: 99%

“…The few studies that have paid any attention to its genetic makeup have focused on its genome structure27 or its mitochondrial DNA inheritance2528 only, but not with the aim to characterise its diversity. However, the availability of highly polymorphic codominant genetic markers, such as microsatellites, allows essential research on the population structure, its effective population size, contemporary phylogeography and conservation genetics of wedge clamps.…”

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confidence: 99%

Implications for management and conservation of the population genetic structure of the wedge clam Donax trunculus across two biogeographic boundaries

Marie

Lejeusne

Karapatsiou

et al. 2016

Sci Rep

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In a resource management perspective, the understanding of the relative influence of the physical factors on species connectivity remains a major challenge and is also of great ecological and conservation biology interest. Despite the overfishing threat on the wedge clam Donax trunculus in Europe, relatively little information is known about its population genetic structure and connectivity and their consequences on conservation policies. We employed 16 microsatellite loci to characterise the genetic diversity and population structure of D. trunculus. A total of 514 samples from seven different localities along the Atlantic-Mediterranean transition, from the Atlantic (Gulf of Cádiz) to the north-western Mediterranean were genotyped. The analysis of the population genetic structure displayed a clear distinction along the Atlantic-Mediterranean transition with different clusters in the Atlantic Ocean, the Alboran Sea and the northwestern Mediterranean. Consequently, we recommend that these three areas should be considered as different management units. We showed that all populations seem to be at high long-term risk of extinction with the exception of the protected Doñana National Park population which still seems to have evolutionary potential. Therefore, our results emphasized the necessity of protection of this economic resource and the validity of molecular tools to evaluate the population dynamics.

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“…To molecularly characterize the studied tellin shells, a fragment of the mitochondrial COI gene was amplified in all karyotyped specimens using the primers developed by Nantón et al [24] for Donacidae.…”

Section: Methodsmentioning

confidence: 99%

Karyotype differentiation in tellin shells (Bivalvia: Tellinidae)

2017

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BackgroundAlthough Tellinidae is one of the largest and most diverse families of bivalves, its taxonomy is utterly chaotic. This is mainly due to the morphological diversity and homoplasy displayed by their shells and to the scarcity of the molecular phylogenetic studies performed on them. A molecular cytogenetic analysis of four tellin shell species, Bosemprella incarnata, Macomangulus tenuis, Moerella donacina and Serratina serrata, was performed. To molecularly characterize the analyzed specimens, the sequence of a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) was also studied.ResultsThe karyotypes of the four species were composed of different amounts of bi-armed and telocentric chromosomes. The chromosomal mapping of 45S and 5S rDNA and H3 histone gene clusters by fluorescent in situ hybridization also revealed conspicuous differences on the distribution of these DNA sequences on their karyotypes. Vertebrate type telomeric sequences were located solely on both ends of each chromosome in all four tellin shells.ConclusionWe present clear evidence of the valuable information provided by FISH signals in both analyzing chromosome evolution in Tellinidae and as a further tool in identifying tellin shell specimens for molecular phylogenies.

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Identification of four Donax species by PCR–RFLP analysis of cytochrome c oxidase subunit I (COI)

Cited by 11 publications

References 24 publications

First complete female mitochondrial genome in four bivalve species genus Donax and their phylogenetic relationships within the Veneroida order

First complete female mitochondrial genome in four bivalve species genus Donax and their phylogenetic relationships within the Veneroida order

Implications for management and conservation of the population genetic structure of the wedge clam Donax trunculus across two biogeographic boundaries

Karyotype differentiation in tellin shells (Bivalvia: Tellinidae)

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