Identification of four type I IFNs from Japanese eel with differential expression properties and Mx promoter inducibility

Huang, Bei; Wang, Z.X.; Liang, Ying; Zhai, Shaowei; Huang, Wen; Nie, Pin

doi:10.1016/j.dci.2018.09.012

Cited by 12 publications

(2 citation statements)

References 86 publications

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“…The generation of type I interferon (IFN) in mammals is induced by the phosphorylation of IRF3 and/or IRF7, which results in their dimerization and nuclear translocation [33]. Huang et al [34] cloned four type I IFN (IFN1, 2, 3, 4) from Japanese eel and found that overexpression of IFN1-4 induced the luciferase activity of the Mx gene reporter. Hence, AjCRFB5a positively regulates host antiviral immune responses through the activation of IRF3 and IRF7-mediated type I IFN signaling pathways, resulting in the expression of Mx proteins or other antiviral proteins.…”

Section: Discussionmentioning

confidence: 99%

CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

Wang,

Lin,

Wang

et al. 2023

Animals

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IFNAR1, one of the type I IFN receptors, is crucial to mammalian host defense against viral invasion. However, largely unknown is the immunological role of the fish teleost protein IFNAR1, also known as CRFB5. We have successfully cloned the whole cDNA of the Japanese eel’s (Anguilla japonica) CRFB5a homolog, AjCRFB5a. The two fibronectin-3 domains and the transmembrane region (238–260 aa) of AjCRFB5a are normally present, and it shares a three-dimensional structure with zebrafish, Asian arowana, and humans. According to expression analyses, AjCRFB5a is highly expressed in all tissues found, particularly the liver and intestine. In vivo, Aeromonas hydrophila, LPS, and the viral mimic poly I:C all dramatically increased AjCRFB5a expression in the liver. Japanese eel liver cells were reported to express AjCRFB5a more strongly in vitro after being exposed to Aeromonas hydrophila or being stimulated with poly I: C. The membranes of Japanese eel liver cells contained EGFP-AjCRFB5a proteins, some of which were condensed, according to the results of fluorescence microscopy. Luciferase reporter assays showed that AjCRFB5a overexpression strongly increased the expression of immune-related genes in Japanese eel liver cells, such as IFN1, IFN2, IFN3, IFN4, IRF3, IRF5, and IRF7 of the type I IFN signaling pathway, as well as one of the essential antimicrobial peptides LEAP2, in addition to significantly inducing human IFN-promoter activities in HEK293 cells. Additionally, RNA interference (RNAi) data demonstrated that knocking down AjCRFB5a caused all eight of those genes to drastically lower their expression in Japanese eel liver cells, as well as to variable degrees in the kidney, spleen, liver, and intestine. Our findings together showed that AjCRFB5a participates in the host immune response to bacterial infection by inducing antimicrobial peptides mediated by LEAP2 and favorably modulates host antiviral immune responses by activating IRF3 and IRF7-driven type I IFN signaling pathways.

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Section: Discussionmentioning

confidence: 99%

CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

Wang,

Lin,

Wang

et al. 2023

Animals

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“…previously (Lutfalla et al [10] (tetraodon); Casani et al [11] (sea bass); Kitao et al [12] (carp); Pereira et al [13] (turbot); Maekawa et al [14] (medaka); Hu et al [15] (flounder); Ding et al [16] (croaker); Huang et al [17] (Japanese eel)), the exact number of each subgroup present and their genomic location were not typically available. Data for the IFN loci/genes in zebrafish (Danio rerio) and stickleback (Gasterosteus aculeatus) were already available and included without further analysis [8].…”

Section: Teleost Fish Genomesmentioning

confidence: 99%

Evolution of IFN subgroups in bony fish - 2. analysis of subgroup appearance and expansion in teleost fish with a focus on salmonids

Liu

Wang

Petit

et al. 2020

Fish & Shellfish Immunology

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Cytokines and Immunity

Secombes

2022

Principles of Fish Immunology

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Identification of four type I IFNs from Japanese eel with differential expression properties and Mx promoter inducibility

Cited by 12 publications

References 86 publications

CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

CRFB5a, a Subtype of Japanese Eel (Anguilla japonica) Type I IFN Receptor, Regulates Host Antiviral and Antimicrobial Functions through Activation of IRF3/IRF7 and LEAP2

Evolution of IFN subgroups in bony fish - 2. analysis of subgroup appearance and expansion in teleost fish with a focus on salmonids

Cytokines and Immunity

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