Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L.)

Suo, Jinfeng; Liang, Xiaoe; Pu, Li; Zhang, Yansheng; Xue, Yongbiao

doi:10.1016/j.bbaexp.2003.08.009

Cited by 156 publications

(153 citation statements)

References 40 publications

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“…Likewise, transcription factors participating in cotton fiber development have been reported by several studies. For example, a few MYB transcription factors were found to play important roles in cotton fiber initiation (Cedroni et al, 2003;Suo et al, 2003;Wang et al, 2004;Pu et al, 2008;Machado et al, 2009;Guan et al, 2011;Walford et al, 2011). In addition, it was recently reported that GbTCP (from sea-island cotton), which shares 47% protein sequence identity with GhTCP14, is involved in cotton fiber development, possibly through JA signaling (Hao et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

The Cotton Transcription Factor TCP14 Functions in Auxin-Mediated Epidermal Cell Differentiation and Elongation

Wang

Zhao²,

Cheng³

et al. 2013

Plant Physiology

112

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Plant-specific TEOSINTE-BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors play crucial roles in development, but their functional mechanisms remain largely unknown. Here, we characterized the cellular functions of the class I TCP transcription factor GhTCP14 from upland cotton (Gossypium hirsutum). GhTCP14 is expressed predominantly in fiber cells, especially at the initiation and elongation stages of development, and its expression increased in response to exogenous auxin. Induced heterologous overexpression of GhTCP14 in Arabidopsis (Arabidopsis thaliana) enhanced initiation and elongation of trichomes and root hairs. In addition, root gravitropism was severely affected, similar to mutant of the auxin efflux carrier PIN-FORMED2 (PIN2) gene. Examination of auxin distribution in GhTCP14-expressing Arabidopsis by observation of auxin-responsive reporters revealed substantial alterations in auxin distribution in sepal trichomes and root cortical regions. Consistent with these changes, expression of the auxin uptake carrier AUXIN1 (AUX1) was up-regulated and PIN2 expression was down-regulated in the GhTCP14-expressing plants. The association of GhTCP14 with auxin responses was also evidenced by the enhanced expression of auxin response gene IAA3, a gene in the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) family. Electrophoretic mobility shift assays showed that GhTCP14 bound the promoters of PIN2, IAA3, and AUX1, and transactivation assays indicated that GhTCP14 had transcription activation activity. Taken together, these results demonstrate that GhTCP14 is a dual-function transcription factor able to positively or negatively regulate expression of auxin response and transporter genes, thus potentially acting as a crucial regulator in auxin-mediated differentiation and elongation of cotton fiber cells.

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Section: Discussionmentioning

confidence: 99%

The Cotton Transcription Factor TCP14 Functions in Auxin-Mediated Epidermal Cell Differentiation and Elongation

Wang

Zhao²,

Cheng³

et al. 2013

Plant Physiology

112

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“…GhMYB109 is present as a uniquecopy gene in cotton genome and RNA expression analysis showed that it was specifically expressed in cotton fibre initial cells as well as elongating fibres (Suo et al 2003). Wang et al (2010) revealed distinct relationships between GL2 and single MYBs in the regulation of trichome vs. root hair development which could provide new insights into the molecular mechanism of epidermal patterning.…”

Section: Trichome Developmentmentioning

confidence: 97%

“…In cotton, GhMYB109 was specifically expressed in cotton fiber initial cells as well as elongating fibers (Suo et al 2003). GhMYB7 and GhMYB9 were expressed in flowers and fibers, and their expression in fibers is developmentally regulated (Chuan et al 2005).…”

Section: Evolution Of Mybmentioning

confidence: 99%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

838

544

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Regulation of gene expression at the level of transcription controls many crucial biological processes. Transcription factors (TFs) play a great role in controlling cellular processes and MYB TF family is large and involved in controlling various processes like responses to biotic and abiotic stresses, development, differentiation, metabolism, defense etc. Here, we review MYB TFs with particular emphasis on their role in controlling different biological processes. This will provide valuable insights in understanding regulatory networks and associated functions to develop strategies for crop improvement.

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“…Therefore, deciphering the molecular control of fiber development will be important for cotton improvement by genetic engineering. In this study, we examined the role of GhMYB109 (Suo et al 2003), similar to AtGL1/WER, in cotton fiber development using a reverse genetics approach. Our results provide an insight into the molecular mechanism regulating cotton fiber development and reveal a largely conserved mechanism in cell fate determination in plants.…”

mentioning

confidence: 99%

The R2R3 MYB Transcription Factor GhMYB109 Is Required for Cotton Fiber Development

et al. 2008

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Cotton (Gossypium hirsutum L.) fibers are single highly elongated cells derived from the outer epidermis of ovules. A large number of genes are required for fiber differentiation and development, but so far, little is known about how these genes control and regulate the process of fiber development. Here we examine the role of the cotton-fiber-specific R2R3 MYB gene GhMYB109 in cotton fiber development. Transgenic reporter gene analysis revealed that a 2-kb GhMYB109 promoter was sufficient to confirm its fiber-specific expression. Antisense-mediated suppression of GhMYB109 led to a substantial reduction in fiber length. Consistently, several genes related to cotton fiber growth were found to be significantly reduced in the transgenic cotton. Our results showed that GhMYB109 is required for cotton fiber development and reveal a largely conserved mechanism of the R2R3 MYB transcription factor in cell fate determination in plants.

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Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L.)

Cited by 156 publications

References 40 publications

The Cotton Transcription Factor TCP14 Functions in Auxin-Mediated Epidermal Cell Differentiation and Elongation

The Cotton Transcription Factor TCP14 Functions in Auxin-Mediated Epidermal Cell Differentiation and Elongation

MYB transcription factor genes as regulators for plant responses: an overview

The R2R3 MYB Transcription Factor GhMYB109 Is Required for Cotton Fiber Development

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