Identification of Hepatitis B Surface Antigen Variants with Alterations outside the “a” Determinant in Immunized Singapore Infants

Oon, Chong Jin; Chen, Ning; S, Koh; Keow, Lim Gek

doi:10.1086/314553

Cited by 71 publications

(28 citation statements)

References 13 publications

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“…Such mutants have also been reported in association with escape from vaccineinduced immunity [Oon et al, 1995;Carman, 1997;Ho et al, 1998;Chong-Jin et al, 1999;He et al, 2001;Lee et al, 2001;Seddigh-Tonekaboni et al, 2001] and with failures of therapy with specific immunoglobulin in newborns from carrier women and in liver-transplant recipients [Harrison et al, 1994;Oon et al, 1996Oon et al, , 2002Brind et al, 1997;Carman, 1997;Ngui et al, 1997;Protzer-Knolle et al, 1998;Santantonio et al, 1999;Roznovsky et al, 2000;Liu et al, 2002]. The amino-acid substitution Gly145Arg has been reported most frequently [Francois et al, 2001], and is known to be stable [Zuckerman and Zuckerman, 1999].…”

Section: Introductionmentioning

confidence: 96%

Frequency of hepatitis B virus ‘a’ determinant variants in unselected Spanish chronic carriers

Avellón

Echevarría

2005

Journal of Medical Virology

101

125

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The prevalence in the population of hepatitis B virus (HBV) surface antigen (HBsAg) variants that may impair diagnosis, or allow the virus to escape vaccine‐induced immunity or passive immunoglobulin therapy is unknown. A genome fragment encoding HBsAg amino acids 112–212 was amplified and sequenced from the sera of 272 unselected DNA‐positive, HBV‐chronic carriers from Spain. The genotype and the HBsAg subtype were predicted from the sequences. Analysis of amino‐acid positions 112–157 revealed single or multiple substitutions in 39% of the carriers studied. Mutations were not detected for residues 121, 135, 137, 139, 140, 141, 142, 146, 147, 148, 149, 151, 152, 153, 155, 156, and 157. Substitutions reported previously to be in association with failures of diagnostic tests or with vaccine or immunoglobulin therapy escape were found in 12.5%, 6.6%, and 9.2% of carriers, respectively. Met133Thr (2.2%); Gln129His, Met133Ile, Phe/Tyr134Asn (1.8%); Phe/Tyr134Leu, Gly145Ala (1.5%), and Pro120Thr (1.1%) were the most frequent. Other substitutions, including Gly145Arg (0.4%), were found at a frequency of less than 1%. Samples containing HBV mutants were tested with three commercial assays for HBsAg screening. Almost all the mutants reacted to the upper cut‐off values of the assays, but six samples with weak reactivity with one or more of the methods were also found. Thus, HBV mutants with a potential impact on clinical and public health issues are moderately frequent among chronic carriers from Spain, although their influence on the performance of diagnostic tests seems to be slight. J. Med. Virol. 78:24–36, 2006. © 2005 Wiley‐Liss, inc.

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Section: Introductionmentioning

confidence: 96%

Frequency of hepatitis B virus ‘a’ determinant variants in unselected Spanish chronic carriers

Avellón

Echevarría

2005

Journal of Medical Virology

101

125

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“…These mutants may lead to reinfection, because it replicates through an RNA intermediate synthesized by reverse transcriptase of viral genomes (Kreutz, 2002;Ohishi et al, 2004) and quasi-species are generated (Torresi, 2002;Liu et al, 2002;Hsu et al, 2004). This resulted in the production of viral mutants during naturally occurring infections (Chong-Jin et al, 1999). Alterations of the structure of HBsAg can disrupt the binding ability of polyclonal antibodies to it, because they contain several epitopes for T or B cells.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel D11 Hepatitis B Surface Antigen Subgenotype in Jeddah, Kingdom of Saudi Arabia

Hadad¹,

Alakilli²,

Rabah³

et al. 2015

Biotechnology

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A B S T R A C T Hepatitis B virus (HBV) infection remains to be a worldwide health problem. In Saudi Arabia, HBV is the most predominant type of hepatitis followed by hepatitis C and hepatitis A while little is known about the molecular epidemiology of the prevalence of HBV genotype/subgenotype particularly in Jeddah. Serum samples were collected from HBV chronic patients and subjected to HBsAg gene amplification. Sequencing and phylogenetic analysis of the entire HBsAg gene sequences revealed that 11 isolates belonged to HBV/D while 4 isolates were associated with HBV/C. Interestingly, HBV/D subgenotypes identified eight HBV/D present isolates belonged to HBV/D1 while three isolates showed a new cluster supporting by a branch with 99% bootstrap value and 4.3-5.8% nucleotide divergence over the entire HBsAg gene from other known subgenotypes D1 to D10, despite they were appearing more related to HBV/D5. The three strains of the new D subgenotype showed unique amino acid sequences consisting of Thr7non, 75Pro in the preS1 gene, 112Ile, 161Gly in the preS2 gene and 196Glu, 197Ala, 238Ser, 259Cys in the S gene. In addition to three amino acid residues in the S gene (373Ile, 374Ala and 381Thr) were specified S118S isolate. Subsequently, it have been verified that HBV/D1 is the most prevalent HBV subgenotype in Jeddah as well as we proposed a novel subgenotype designated HBV/D11. The identification of HBV/D11 novel subgenotypes in the present study suggested that further studies with a large number of subjects in previously examined and unexamined areas may lead to discovering new HBV strain genotypes and/or subgenotypes circulating in Saudi Arabia.

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“…In Vietnam, three M133L mutations have been found in the first loop of the "a" determinant, no mutation was identified within the second loop, while T126M was found in one sample (Thuy et al, 2005). In Singapore, HBsAg mutation was found in neonates of HBsAg positive mothers; 16 (39%) of 41 neonates showed breakthrough infection despite HBIg and a full course of a plasma-derived vaccine being given (Chong-Jin et al, 1999). However, in Thailand, 22.4% of vaccinated and unvaccinated children were found to have varying mutations in the "a" determinant region of HBV (Poovorawan et al, 2012).…”

Section: Hepatitis B Surface Mutationmentioning

confidence: 99%

An Overview of Hepatitis B Virus Surface Antigen Mutant in the Asia Pacific

Hudu

Malik²,

Taib³

et al. 2014

Current Issues in Molecular Biology

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Hepatitis B virus infection is a serious health problem worldwide, and more than 350 million people are chronic carriers, constituting a major global threat. Southeast Asia and the Western Pacific have the highest levels of endemicity in the world, with an estimated seroprevalence ranging between 2% and 31%. Mutations in the hepatitis B surface antigen (HBsAg) have been reported in many parts of the world but are most common in Asian infants; such mutants have several clinical effects, such as the development of hepatocellular carcinoma. Diagnostic failures by commercial assays have reduced the diagnostic effectiveness of HBsAg detection. For example the substitution of an amino acid in the major hydrophilic region of the S gene reduces the binding of hepatitis B surface antibodies leading to immune escape. The safety of blood transfusion may be compromised by current screening tests due to escape from being neutralised by antibodies induced by HBsAg mutants, and undetectable levels of viral surface protein. Data on the epidemiology of HBsAg mutation in Asia Pacific are scant; however, this manuscript has reviewed the available information on the epidemiology of HBsAg mutation in Asia Pacific.

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Identification of Hepatitis B Surface Antigen Variants with Alterations outside the “a” Determinant in Immunized Singapore Infants

Cited by 71 publications

References 13 publications

Frequency of hepatitis B virus ‘a’ determinant variants in unselected Spanish chronic carriers

Frequency of hepatitis B virus ‘a’ determinant variants in unselected Spanish chronic carriers

Identification of Novel D11 Hepatitis B Surface Antigen Subgenotype in Jeddah, Kingdom of Saudi Arabia

An Overview of Hepatitis B Virus Surface Antigen Mutant in the Asia Pacific

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