Identification of IgG-κ type macroprolactin found in the serum of an 8-year-old girl

Transient human anti-mouse antibody generated with immune enhancement in a carbohydrate antigen 19-9 immunoassay after surgical resection of recurrent cancer

“…Size-exclusion HPLC was carried out on a Superose 12 column (10 Â 300 mm, GE Healthcare, Buckinghamshire, England) as previously described. 14 Briefly, 200 mL of serum were injected. The elution buffer was 150 mM NaCl and 16 mM phosphate buffer (pH 7.4).…”

“…Immunoprecipitation and the addition of mouse immunoglobulins were performed using polyclonal anti-human IgM 14,17 and mouse immunoglobulins, using ST AIA-PACK Myoglobin (TOSHO), respectively. One hundred microliters of antiserum or mouse antibody was mixed with 100 mL of the patient's serum and incubated at 4 C overnight in the immunoprecipitation reaction.…”

“…Covinsky et al 12 demonstrated that an IgM lambda antibody to Escherichia coli produces false-positive results in multiple immunometric assays. Moreover, immunoglobulin binds to hormones, macro prolactin, 13,14 macro thyroidstimulating hormone, 15 and macro follicle-stimulating hormone, 16 resulting in incorrect clinical interpretation due to low or zero bioactivity. Therefore, it is important to take into account this possibility when the laboratory results disagree with the clinical course.…”

Laboratory and clinical features of abnormal macroenzymes found in human sera

“…Unfortunately, that is not fully understood at present. However, immune complexes with numerous serum bioactive substances have been also reported in recent years [135,[196][197][198][199][200][201][202][203][204][205][206][207][208]. For example, blood coagulation factor, various hormones (triiodothyroxine, thyroxine, chorionic gonadotropin, pituitary hormones and so on), vitamin B12, myocardial markers and so on complexed with immunoglobulin.…”