Identification of immunoreactive proteins from the dog heartworm (Dirofilaria immitis) differentially recognized by the sera from dogs with patent or occult infections

Oleaga, Ana; Pérez–Sánchez, Ricardo; Pagés, Elaine; Marcos‐Atxutegi, C.; Simón, F.

doi:10.1016/j.molbiopara.2009.03.005

Cited by 24 publications

(31 citation statements)

References 41 publications

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“…Together with mass spectrometry, proteomics allowed the simultaneous identification of numerous proteins, comprising 39 proteins from D. immitis and 15 from D. repens, many of which were represented by several isoforms (157,158,313). These proteins belong primarily to four functional groups, including metabolic enzymes, enzymes with redox or detoxification potential, and molecules involved in motility and the stress response ( Table 1).…”

Section: Classical and Proteomic Approaches To The Study Of Proteins mentioning

confidence: 99%

“…Many glycolytic enzymes have been found in D. immitis (70). The presence of several isoforms of some of those glycolytic enzymes has been demonstrated for both D. immitis and D. repens (158,313), which could provide parasites with an increased ability to thwart any host interference in the energy generation processes required for parasite survival. In contrast, the aerobic metabolism of carbohydrates predominates in microfilariae (188), and oxygen is required for motility although apparently not for survival (207).…”

Section: Energy Generation and Molting In Dirofilaria Speciesmentioning

confidence: 99%

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Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic

et al. 2012

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SUMMARY Dirofilariasis represents a zoonotic mosaic, which includes two main filarial species ( Dirofilaria immitis and D. repens ) that have adapted to canine, feline, and human hosts with distinct biological and clinical implications. At the same time, both D. immitis and D. repens are themselves hosts to symbiotic bacteria of the genus Wolbachia , the study of which has resulted in a profound shift in the understanding of filarial biology, the mechanisms of the pathologies that they produce in their hosts, and issues related to dirofilariasis treatment. Moreover, because dirofilariasis is a vector-borne transmitted disease, their distribution and infection rates have undergone significant modifications influenced by global climate change. Despite advances in our knowledge of D. immitis and D. repens and the pathologies that they inflict on different hosts, there are still many unknown aspects of dirofilariasis. This review is focused on human and animal dirofilariasis, including the basic morphology, biology, protein composition, and metabolism of Dirofilaria species; the climate and human behavioral factors that influence distribution dynamics; the disease pathology; the host-parasite relationship; the mechanisms involved in parasite survival; the immune response and pathogenesis; and the clinical management of human and animal infections.

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Section: Classical and Proteomic Approaches To The Study Of Proteins mentioning

confidence: 99%

Section: Energy Generation and Molting In Dirofilaria Speciesmentioning

confidence: 99%

Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic

et al. 2012

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“…Sequence analysis indicated that Di-iPGM and wDi-iPGM share a high degree of amino acid identity with the well-characterized iPGM from B. stearothermophilus and possess all 15 residues predicted to be involved in catalysis, including the catalytic serine (Jedrzejas et al, 2000a,b). Interestingly, in recent proteomic studies using heartworm extracts, peptides likely corresponding to iPGM were found to react with sera from dogs (Oleaga et al, 2009) and cats (González-Miguel et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of the cofactor-independent phosphoglycerate mutases of Dirofilaria immitis and its Wolbachia endosymbiont

Galvin

Raverdy

et al. 2011

Veterinary Parasitology

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“…A number of diverse native and recombinant antigens have been used in serological diagnostic tests [10][11][12] ; however, it has been revealed that the recombinant antigens are more sensitive and specific than crude antigens [13] . The major surface antigen of D. immitis infective larvae, previously characterized in its native form [14] , has already been produced as a recombinant antigen [15] .…”

Section: Introductionmentioning

confidence: 99%

Development of New Recombinant DgK Antigen for Diagnosis of Dirofilaria immitis Infections in Dogs Using ELISA Technique and Its Comparison to Molecular Methods

Solgi¹,

Sadjjadi²,

Mohebali³

et al. 2018

IBJ

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Background: Dirofilaria immitis is a cosmopolitan zoonotic, vector-borne parasite of carnivorous animals causing dirofilariasis in human beings. Common commercial serodiagnostic tests for canine dirofilariasis usually lead to different results in their sensitivity and specificity. The present study reports development of recombinant DgK (rDgK) antigen of D. immitis for accurate immunodiagnosis of D. Immitis-infected dogs using indirect ELISA test. Methods: The rDgK coding sequence was successfully sequenced, codon optimized and cloned in pET-24a(+) expression vector and then expressed in Escherichia coli. The recombinant DgK was affinity purified using Ni² + -charged HiTrap chelating column, followed by testing in Western blotting and enzyme-linked immunosorbent assays (ELISA) with dog sera from a dirofilariasis endemic area. The performance of rDgK ELISA was evaluated using 60 sera collected from suspected dogs, while molecular technique was used as a reference test. Results: Sera from positive control D. immitis infection produced a strong IgG antibody response to rDgK both in ELISA and Western blotting tests. The sensitivity and specificity related to diagnostic potential of rDgK for ELISA were 92.5% and 87.5%, respectively. The results of rDgK ELISA showed a high agreement (0.764) with molecular identification. Conclusions: The findings revealed that the developed new rDgK antigen is sensitive and specific for immunodiagnosis of canine dirofilariasis using ELISA test.

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Identification of immunoreactive proteins from the dog heartworm (Dirofilaria immitis) differentially recognized by the sera from dogs with patent or occult infections

Cited by 24 publications

References 41 publications

Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic

Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic

Identification and characterization of the cofactor-independent phosphoglycerate mutases of Dirofilaria immitis and its Wolbachia endosymbiont

Development of New Recombinant DgK Antigen for Diagnosis of Dirofilaria immitis Infections in Dogs Using ELISA Technique and Its Comparison to Molecular Methods

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