Identification of Infectious Diseases Patterns in the Combined Use of Bacteriological Diagnostics and MALDI Biotyper

Voropaeva, N. M.; Belkova, Natalia; Nemchenko, U. M.; Grigorova, E. V.; Kungurtseva, E. A.; Носкова, О. А.; Chemezova, N. N.; Савилов, Е. Д.

doi:10.29413/abs.2020-5.6.10

Acta biomedica scientifica

2021

DOI: 10.29413/abs.2020-5.6.10

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Identification of Infectious Diseases Patterns in the Combined Use of Bacteriological Diagnostics and MALDI Biotyper

N. M. Voropaeva

Natalia Belkova

U. M. Nemchenko

et al.

Abstract: В многопрофильных стационарах имеются условия, способствующие возникновению инфекций, связанных с оказанием медицинской помощи: высокая концентрация лиц со сниженным иммунитетом на ограниченной территории, наличие значительного числа источников инфекции (больных и носителей), изменение биоценоза слизистых оболочек и кожных покровов у пациентов и медицинского персонала под влиянием широкого применения антибиотиков и цитостатиков. Цель исследования состояла в сопоставлении стандартизированных бактериологических … Show more

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Cited by 2 publications

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Biological properties and genetic structure of clinic isolates of Klebsiella pneumoniae species

Belkova,

Klimenko,

Nemchenko

et al. 2024

Acta biomedica scientifica

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Klebsiella pneumoniae (Kp) species complex is a genetically and ecologically diverse group of bacteria that causes a wide range of infections in humans and animals.The aim. To carry out biological characterization and genotyping based on the study of different loci of Klebsiella pneumoniae clinical isolates.Materials and methods. The object of the study was three Klebsiella pneumoniae clinical isolates from different biotopes of patients from a regional children’s multidisciplinary hospital. We used a complex of bacteriological, molecular genetic and bioinformatic methods. Genotyping of the isolates was carried out using the Pasteur Institute service for strains of the K. pneumoniae species complex.Results. All strains were sensitive to antimicrobial drugs from carbapenem (imipenem, meropenem) and tetracycline groups (tigecycline), and demonstrated high sensitivity to the Klebsiella polyvalent bacteriophage. The antibiotic resistance of the Kp ODKB-16 and ODKB-81 isolates to seven and eight antimicrobial drugs, respectively, was registeredBased on the results of multi-locus sequence typing, all strains were assigned to Kp1 phylogroup, K2 type and differed in sequence type, scgMLST629 profile and KL type. Kp ODKB-16 strain was identified as ST-65, scgST-11107, KL2; ODKB-07 strain – as ST-219, scgST-6401, KL125KL114; ODKB-81 strain – as ST-86, scgST-2800, KL2KL30. The virulence gene clusters AbST, CbST, YbST, SmST, and RmST have been characterized only in the genome of the Kp ODKB-16 isolate, allowing it to be characterized as highly virulent with multidrug resistance (MDR). Additionally, genes responsible for the synthesis of types 1 and 3 fimbrial adhesins were registered in all strains, and ter operon loci were identified only in Kp ODKB-16. Resistome analysis showed that all strains had 2b genotype. Plasmids were found in the genomes of Kp ODKB-81 (IncI2) and ODKB-16 (IncFIA + IncFIB + IncHI1B).Conclusion. We used a comprehensive framework for genomic taxonomy of clinical isolates, which can contribute to the unification of global and regional peculiarities of the developing and microevolution of bacterial pathogens.

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Biological properties and genetic structure of clinic isolates of Klebsiella pneumoniae species

Belkova,

Klimenko,

Nemchenko

et al. 2024

Acta biomedica scientifica

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Structure and Antibiotic Resistance of the Main Causative agents of Infections Associated with the Provision of Medical care

Voropaeva

Nemchenko

Grigorova

et al. 2023

Epidemiology and Vaccinal Prevention

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Relevance. The problem of healthcare-associated infections (HCAI) is becoming increasingly important in epidemiological, social and economic terms. The most serious threat to hospital patients is multidrug-resistant Gram-negative organisms such as Pseudomonas aeruginosa and Klebsiella pneumoniae. The purpose of the study was to determine the etiological structure and sensitivity to antimicrobial agents of infectious agents associated with the provision of medical care in a children's multidisciplinary hospital. Materials and methods. The strains used in the work were obtained from patients aged from one to 15 years with severe infectious diseases. Additionally, swabs were taken from environmental objects. Bacteriological identification of selected strains was performed using standardized bacteriological algorithms and MALDI-TOF direct protein profiling of non-spore-forming microorganisms. Results. P. aeruginosa and K. pneumoniae were identified as the main causative agents of HAI with multiple resistance to AMPs in the children's multidisciplinary hospital, the source of which was mainly the respiratory tract. Multiple resistance to AMP was higher in K. pneumoniae, 38.9% of the studied isolates showed resistance to four drugs simultaneously. P. aeruginosa in 23.1% of cases was resistant to one AMP, in 15.4% - to two, four and seven AMPs. Conclusion. For a reasonable choice and optimization of antibacterial treatment of hospital patients, it is necessary to take into account the fact of wide circulation of hospital strains. Systematic monitoring of the constantly changing microbiological landscape of hospitals based on microbiological monitoring data and determining the levels of resistance to antimicrobial chemotherapy drugs will help reduce the risk and prevent the development of infections associated with healthcare.

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Identification of Infectious Diseases Patterns in the Combined Use of Bacteriological Diagnostics and MALDI Biotyper

Cited by 2 publications

References 13 publications

Biological properties and genetic structure of clinic isolates of Klebsiella pneumoniae species

Biological properties and genetic structure of clinic isolates of Klebsiella pneumoniae species

Structure and Antibiotic Resistance of the Main Causative agents of Infections Associated with the Provision of Medical care

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