IDENTIFICATION OF JAVAN LANGUR (Trachypithecus auratus) IN JAVAN LANGUR CENTER (JLC) COBAN TALUN-BATU BASED ON D-LOOP SEQUENCES

Abstract

Javan Langur (Trachypithecus auratus) is one of endemic primates in Indonesia and classified into vulnerable level. Based on its morphological characteristic and geographic area, Javan Langur divided into 2 subspecies, those are Trachypithecus auratus auratus and Trachypithecus auratus mauritius. The identification of its morphological characteristic and genetic in species or subspecies level is important before releasing because it is correlated with inbreeding grouping and releasing place. The aim of this study is to identify the Javan Langur (Trachypithecus auratus) in Javan Langur Center (JLC) Coban Talun-Batu based on D-Loop sequence. The blood sample used in this study was taken from two adult female Javan Langur named Ijem and Embun, that have different morphological characteristics. Both (primates) are suggested to be T.a.mauritius and T.a.auratus respectively. The amplification of D-Loop sequence by mean of PCR technique was performed using specific designed primers forward D-LoopRs 5'-GTACTTAACTCCACCACCAA-3' and reverse D-LoopRs 5'-GTTGAGTTGGGTATGCTCGA-3'. The phylogenetic tree reconstruction based on MEGA 5 using Maximum Likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model shows that Ijem and Embun are placed in different clade. Pairwise distance from those samples value is 11,4%, it prove that they are interspecies. This result supports the morphological characteristic and previous research used Cyt-b sequence, Ijem is Trachypithecus auratus mauritius and Embun is Trachypithecus auratus auratus.