Identification of Linear B Cell Epitopes on CD2V Protein of African Swine Fever Virus by Monoclonal Antibodies

Rui, Jia; Zhang, Gaiping; Bai, Yilin; Liu, Hongliang; Chen, Yumei; Ding, Peiyang; Zhou, Jingming; Feng, Hao; Li, Mingyang; Tian, Yuanyuan; Wang, Aiping

doi:10.1128/spectrum.01052-21

Cited by 18 publications

(11 citation statements)

References 47 publications

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“…The full identification of ASFV's antigenic epitopes is essential for the creation of targeted serological assays and the development of efficacious subunit vaccines. As of right now, several important antigenic epitopes have been identified, mainly in relation to the proteins p30 ( Zhou et al., 2022 ), p54, CD2v ( Jia et al., 2022 ; Ren et al., 2022 ) and p72 ( Heimerman et al., 2018 ), which have the potential to be targets for serological assays and subunit vaccine components. Among them, p30 protein has garnered significant attention in studies due to its potent immunogenicity and status as the primary early protein of ASFV replication ( Liberti et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel linear B-cell epitope on the p30 protein of African swine fever virus using monoclonal antibodies

Tian,

Sun,

Wang

et al. 2024

Virus Research

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Section: Discussionmentioning

confidence: 99%

Identification of a novel linear B-cell epitope on the p30 protein of African swine fever virus using monoclonal antibodies

Tian,

Sun,

Wang

et al. 2024

Virus Research

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“…The results showed that the affinity of MHC-I and MHC-II for Mink CAdV-1 100 K protein was significantly better than that of Fox CAdV-1 100 K protein. Jia identified CD2v B-cell epitopes using scanning peptides and bioinformatics (Jia et al, 2022 ). By predicting and calculating the potential B and T cell epitopes of several EBV proteins, these proteins could mediate epithelial cell attachment and diffusion, capsid self-assembly, DNA synthesis, and replication (Olotu and Soliman, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

First identification of canine adenovirus 1 in mink and bioinformatics analysis of its 100 K protein

Hou,

Xu,

Wang

et al. 2023

Front. Microbiol.

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IntroductionAnimal trade favors the spreading of emerging canine adenovirus 1 (CAdV-1) in mink. Because the 100K protein is not exposed to the viral surface at any stage, it can be used to differentiate the vaccine from wild virus infection. However, no related research has been conducted. This study aimed to find evidence of CAdV-1 in mink and predict the character of the 100K protein in the current circulating CAdV-1 strain of mink.MethodIn this experiment, the identification of CAdV-1, the phylogenetic tree, homology, and bioinformatics analysis of 100K were conducted.ResultsThe results showed that the CAdV-1 was identified in the mink and that its Fiber was located in a separate branch. It was closely related to strains isolated from Norwegian Arctic fox and Red fox. 100K was located in a separate branch, which had the closest genetic relationship with skunks, porcupines, raccoons, and hedgehogs and a far genetic relationship with the strains in dogs. 100K protein is an unstable and hydrophobic protein. It had evidence of selective pressure and recombination, 1 glycosylation site, 48 phosphorylation sites, 60 dominant B cell epitopes, and 9 peptides of MHC-I and MHC-II. Its subcellular localization was mainly in the endoplasmic reticulum and mitochondria. The binding sites of 100K proteins were DBP proteins and 33K proteins.DiscussionThe stains in the mink were different from fox. The exploration of its genomic characteristics will provide us with a deeper understanding of the prevention of canine adenovirus.

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“…However, the CD2v protein, as the crucial functional antigen, there are few studies on B-cell epitopes. Only several B-cell epitopes have been reported recently (Jia et al, 2022;Ren et al, 2022). Therefore, the extracellular domain of CD2v was selected as the immunogen of immunized animals for the expression and purification procedures in this study.…”

Section: Discussionmentioning

confidence: 99%

Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v

Liu

Ding

et al. 2022

Front. Microbiol.

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African swine fever virus (ASFV), a DNA double-stranded virus with high infectivity and mortality, causing a devastating blow to the pig industry and the world economy. The CD2v protein is an essential immunoprotective protein of ASFV. In this study, we expressed the extracellular region of the CD2v protein in the 293F expression system to achieve proper glycosylation. Monoclonal antibodies (mAbs) were prepared by immunizing mice with the recombinant CD2v protein. Eventually, four mAbs that target the extracellular region of the ASFV CD2v protein were obtained. All four mAbs responded well to the ASFV HLJ/18 strain and recognized the same linear epitope, 154SILE157. The specific shortest amino acid sequence of this epitope has been accurately identified for the first time. Meaningfully, the 154SILE157 epitope was highly conformed in the ASFV Chinese epidemic strain and Georgia2008/1 strains according to the analysis of the conservation and have a fair protective effect. These findings contribute to further understanding of the protein function of CD2v and provide potential support for the development of diagnostic tools and vaccines for ASFV.

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Identification of Linear B Cell Epitopes on CD2V Protein of African Swine Fever Virus by Monoclonal Antibodies

Abstract: The ASFV CD2V protein is a crucial antigen on the outer envelopes of virus particles. A modified ASFV CD2V dimeric protein was expressed in the Bac-to-Bac baculovirus expression vector system.

Cited by 18 publications

References 47 publications

Identification of a novel linear B-cell epitope on the p30 protein of African swine fever virus using monoclonal antibodies

Identification of a novel linear B-cell epitope on the p30 protein of African swine fever virus using monoclonal antibodies

First identification of canine adenovirus 1 in mink and bioinformatics analysis of its 100 K protein

Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v

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