1996
DOI: 10.1016/0014-5793(96)00120-2
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Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization

Abstract: The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin were examined. Reverse transcription-polymerasc chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further conf'wmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyelonal antibody against human recombinant MIF. Immunohistochemical studies showed… Show more

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Cited by 131 publications
(109 citation statements)
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“…Very recently, we reported the presence of MIF in human epidermal keratinocytes and corneal epithelial cells (25,26). In this study, we showed that MIF was expressed in human kidney, using a human kidney tissue specimen and cultured renal tubule epithelial cells.…”
Section: Vol 40 No 6 1996mentioning
confidence: 62%
“…Very recently, we reported the presence of MIF in human epidermal keratinocytes and corneal epithelial cells (25,26). In this study, we showed that MIF was expressed in human kidney, using a human kidney tissue specimen and cultured renal tubule epithelial cells.…”
Section: Vol 40 No 6 1996mentioning
confidence: 62%
“…11,12 Recent studies have shown that MIF may function to regulate cellular differentiation and proliferation in normal and tumor-derived cell lines, and may also contribute to the neovascularization of murine tumors. [13][14][15][16] Accordingly, in an approach to identify candidate genes for the hypoxic tumor phenotype, MIF mRNA was found to be induced by hypoxia in human carcinoma cells, 17 and one investigation detected enhanced levels of MIF transcripts in GBM tissues using microarrays. 18 Mutations of the tumor-suppressor gene p53 were found to be associated with the progression of individual gliomas.…”
mentioning
confidence: 99%
“…In the skin, MIF is expressed in the epidermal keratinocytes and fibroblasts (Shimizu et al, 1996, Watanabe et al, 2004. MIF is known to play an important role in the skin with regard to inflammation, the immune response, cutaneous wound healing (Zhao et al, 2005, Dewor et al, 2007 and skin diseases, such as atopic dermatitis (Shimizu et al, 1999a, Hamasaka et al, 2009).…”
Section: The Role Of Mif In the Skin After Uv Radiationmentioning
confidence: 99%