Identification of Major Cultivable Aerobic Bacteria in the Oral Cavity of Malaysian Subjects

Abstract

Culture dependent and culture independent methods have shown that about 600 species of bacteria inhabit the human oral cavity. While some oral microorganisms have a direct link to dental caries, periodontal disease and halitosis, opportunistic pathogens may be responsible for systemic diseases such as bacterial endocarditis, aspiration pneumonia, osteomyelitis in children, preterm low birth weight, coronary heart disease and cerebral infarction (or stroke). This study employs bacterial 16S rDNA sequences to rapidly identify the major cultivable aerobic bacteria in the oral cavity of Malaysian subjects. The data obtained shows that the oral cavity of healthy volunteers contains a number of potentially pathogenic organisms including Streptococcus pneumoniae and Staphylococcus aureus. The need to profile and characterize these microorganisms using rapid detection methods can go a long way in developing future management strategies in clinical setting to enhance oral health in the Malaysian population.

Oral Bacterial Communities in Individuals with Type 2 Diabetes Who Live in Southern Thailand

“…The frequent occurrence of Streptococcus sanguinis, Actinomyces viscosus, Neisseria subflava, and Lautropia sp. in supragingival plaque in the Thai subjects is broadly in agreement with a previous Malaysian study which similarly reported the presence of Streptococcus oralis, Streptococcus sanguinis, Actinomyces viscosus, Actinomyces naeslundii, Lautropia sp., Kingella oralis, Neisseria subflava, Neisseria mucosa, and Rothia mucilaginosa on the tooth surfaces of healthy persons (34 In the present study, Neisseria flavescens was detected only in dental plaque and not in the saliva of either nondiabetic or diabetic individuals. Neisseria, an obligate aerobe, has been implicated in the early stages of dental plaque development (48).…”

“…This approach has been previously used to profile the composition of oral microbial communities (17,34,35). DGGE analysis has been previously used in a limited number of studies to produce fingerprints of saliva (36,37) and supragingival plaque diversity (38,39), and some of these studies have combined DGGE, cluster analysis, and sequencing.…”

Frictional Properties of Self-Ligating Brackets and Low-Friction Ligatures

“…The magnitude of force during orthodontic treatment will result in optimal tissue response and rapid tooth movement. Therefore orthodontic movement should be impressed with low forces (Berger, 2000), thus ensuring treatment efficiency in respect of biologic principles (Thorstenson and Kusy, 2003), oral flora (Koshy et al, 2008) arch forms (Mohammad et al, 2011) and allowing correct jaw movements (Zhuohua et al, 2010). Friction at the bracket archwire interface might prevent the attainment of optimal force levels in the supporting tissues.…”

An in Vitro Evaluation of the Effectiveness of Gotu Kola ( Centella Asiatica ) on Inhibiting the Growth of Selected Microorganisms in Human Saliva

“…Many microorganisms including Staphylococcus species, Streptococcus species, and Lactobacillus species are found in human saliva (5) . Dental caries has been known to be caused by acidogenic and aciduric bacteria, such as mutans streptococci and lactobacilli (6,7) .…”