Identification of maternal transcripts that progressively disappear during the cleavage period of rabbit embryos

Henrion, Gwénola; Brunet, Adeline; Renard, Jean‐Paul; Duranthon, Véronique

doi:10.1002/(sici)1098-2795(199708)47:4<353::aid-mrd1>3.0.co;2-j

Cited by 30 publications

(8 citation statements)

References 46 publications

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“…Egg activation also induces the destabilization of a subset of maternal transcripts. This phenomenon has been observed in a variety of model organisms such as mouse, rabbit, zebrafish, Xenopus , and Drosophila (Bachvarova and De Leon, 1980; Duval et al, 1990; Henrion et al, 1997, 2000; Bashirullah et al, 1999; Brunet‐Simon et al, 2001; Kishida and Callard, 2001).…”

Section: Posttranscriptional Control During Egg Activation and Early mentioning

confidence: 85%

Setting the stage for development: mRNA translation and stability during oocyte maturation and egg activation in Drosophila

Tadros

Lipshitz

2005

Developmental Dynamics

109

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Early animal development is controlled by maternally encoded RNAs and proteins, which are loaded into the egg during oogenesis. Oocyte maturation and egg activation trigger changes in the translational status and the stability of specific maternal mRNAs. Whereas both maturation and activation have been studied in depth in amphibians and echinoderms, only recently have these processes begun to be dissected using the powerful genetic and molecular tools available in Drosophila. This review focuses on the mechanisms and functions of regulated maternal mRNA translation and stability in Drosophila-and compares these mechanisms with those elucidated in other animal models, particularly Xenopus-beginning late in oogenesis and continuing to the mid-blastula transition, when developmental control is transferred to zygotically synthesized transcripts. Developmental Dynamics 232:593-608, 2005.

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Section: Posttranscriptional Control During Egg Activation and Early mentioning

confidence: 85%

Setting the stage for development: mRNA translation and stability during oocyte maturation and egg activation in Drosophila

Tadros

Lipshitz

2005

Developmental Dynamics

109

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“…In vitro embryos were recovered at the one cell stage (19 h post-coitum) from superovulated females treated as described by Henrion et al . [ 19 ]. They were cultured from the one cell stage onwards (19 h pc ) until the early morula (58 h pc ) and blastocyst (100 h pc ) stages respectively in four different culture media: B2 medium (Laboratoire C.C.D.…”

Section: Methodsmentioning

confidence: 99%

SSH adequacy to preimplantation mammalian development: Scarce specific transcripts cloning despite irregular normalisation

et al. 2005

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Background: SSH has emerged as a widely used technology to identify genes that are differentially regulated between two biological situations. Because it includes a normalisation step, it is used for preference to clone low abundance differentially expressed transcripts. It does not require previous sequence knowledge and may start from PCR amplified cDNAs. It is thus particularly well suited to biological situations where specific genes are expressed and tiny amounts of RNA are available. This is the case during early mammalian embryo development. In this field, few differentially expressed genes have been characterized from SSH libraries, but an overall assessment of the quality of SSH libraries is still required. Because we are interested in the more systematic establishment of SSH libraries from early embryos, we have developed a simple and reliable strategy based on reporter transcript follow-up to check SSH library quality and repeatability when starting with small amounts of RNA.

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“…New Zealand White female rabbits ( Oryctolagus cuniculus ) were superovulated as described by Henrion et al (1997) and mated with normal males (giving rise to wild-type embryos) or vasectomized males (for clones). One-cell stage embryos were flushed from oviducts with PBS at 19 h postcoitum (hpc).…”

Section: Methodsmentioning

confidence: 99%

Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos

et al. 2018

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Mammalian embryo cloning by nuclear transfer has a low success rate. This is hypothesized to correlate with a high variability of early developmental steps that segregate outer cells, which are fated to extra-embryonic tissues, from inner cells, which give rise to the embryo proper. Exploring the cell lineage of wild-type embryos and clones, imaged in toto until hatching, highlights the respective contributions of cell proliferation, death and asymmetric divisions to phenotypic variability. Preferential cell death of inner cells in clones, probably pertaining to the epigenetic plasticity of the transferred nucleus, is identified as a major difference with effects on the proportion of inner cell. In wild type and clones, similar patterns of outer cell asymmetric divisions are shown to be essential to the robust proportion of inner cells observed in wild type. Asymmetric inner cell division, which is not described in mice, is identified as a regulator of the proportion of inner cells and likely gives rise to resilient clones.

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Identification of maternal transcripts that progressively disappear during the cleavage period of rabbit embryos

Cited by 30 publications

References 46 publications

Setting the stage for development: mRNA translation and stability during oocyte maturation and egg activation in Drosophila

Setting the stage for development: mRNA translation and stability during oocyte maturation and egg activation in Drosophila

SSH adequacy to preimplantation mammalian development: Scarce specific transcripts cloning despite irregular normalisation

Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos

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