Identification of Medically Important Candida Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of the rDNA ITS1 and ITS2 Regions

Bayraktar, Suphi; Duran, Nizami; Duran, Gülay Gülbol; Eryilmaz, Naciye; Aslan, Hayat; Önlen, Cansu; Özer, Burçin

doi:10.4103/ijmm.ijmm_17_102

Cited by 7 publications

(11 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aslani et al have also reported that C. albicans isolates are highly resistant to ITC, VRC, and FLU [ 64 ]. According to the findings of previous studies, resistance to azoles in C. glabrata has a multifactorial nature [ 65 ]. Based on the results of a study conducted by Amirrajab N. et al [ 66 ], the overall rates of resistance to ITC, VRC, and FLU were 72.5%, 47.5%, and 10% respectively, which are inconsistent with those of the present study.…”

Section: Discussionmentioning

confidence: 99%

High prevalence of asymptomatic nosocomial candiduria due to Candida glabrata among hospitalized patients with heart failure: a matter of some concern?

Âghili¹,

Abastabar²,

Soleimani³

et al. 2021

CMM

View full text Add to dashboard Cite

Background and Purpose: Heart failure is a leading cause of hospitalization, and asymptomatic candiduria is common in hospitalized patients with low morbidity. However, in most patients, it is resolved spontaneously on the removal of the catheter. Despite the publication of guidelines, there are still controversies over the diagnosis and management of candiduria. However, in hospitalized patients with heart failure, the decision to treat candiduria is especially important since the nosocomial infections are associated with an increase in morbidity, mortality, length of hospital stay, and healthcare costs. Some species of Candida, such as Candida glabrata, are increasingly resistant to the first-line and second-line antifungal medications. The present study aimed to investigate the incidence of asymptomatic Candida urinary tract infection due to C. glabrata and antifungal susceptibility of Candida isolates in hospitalized patients with heart failure. Materials and Methods: In total, 305 hospitalized patients with heart failure were studied to identify asymptomatic nosocomial candiduria during 2016-17 in one private hospital in the north of Iran. The Sabouraud’s dextrose agar culture plates with a colony count of >104 colony-forming unit/ml of urine sample were considered as Candida urinary tract infection. Candida species were identified based on the morphology of CHROMagar Candida (manufactured by CHROMagar, France) and PCR-RFLP method with MspI restriction enzyme. Antifungal susceptibility testing of the isolates was performed using five mediations, including itraconazole, voriconazole, fluconazole, amphotericin B, and caspofungin by broth microdilution method according to CLSI M27-S4. Results: In this study, the rate of asymptomatic Candida urinary tract infection was 18.8%, which was more common in people above 51 years old and females (70%). In addition to the urinary and intravascular catheter, the occurrence of candiduria in hospitalized patients had significant relationships with a history of surgical intervention, diastolic heart failure, and use of systemic antibiotics (P>0.05). Among Candida spp., non-albicans Candida species was the most common infectious agent (59.7%). Moreover, C. glabrata (n=27, 40.3%) (alone or with other species) and Candida albicans (n=27, 40.3%) were the most common agents isolated in Candida urinary tract infection. Based on the results of the in vitro susceptibility test, the C. glabrata isolates were 15%, 59%, 70%, 74%, and 85% susceptible to caspofungin, amphotericin B, itraconazole, voriconazole, and fluconazole, respectively. Conclusion: According to the findings, there was a high prevalence of asymptomatic Candida urinary tract infection in hospitalized patients with heart failure. Besides, it was suggested that there was a shift towards non-albicans Candida, especially C. glabrata, in these patients. Therefore, asymptomatic candiduria in hospitalized patients with heart failure should be considered significant. Furthermore, the identification of Candida species along with antifungal susceptibility is essential and helps the clinicians to select the appropriate antifungal agent for better management of such cases.

show abstract

Section: Discussionmentioning

confidence: 99%

High prevalence of asymptomatic nosocomial candiduria due to Candida glabrata among hospitalized patients with heart failure: a matter of some concern?

Âghili¹,

Abastabar²,

Soleimani³

et al. 2021

CMM

View full text Add to dashboard Cite

show abstract

“…The most important mechanism of resistance to penicillin is beta-lactamase production, which inactivates penicillin by hydrolysis of its beta-lactam ring. Another mechanism is associated with penicillin-binding protein 2a (PBP2a), encoded by mecA [9]. MRSA strains carry the mecA gene, which is part of a larger piece of foreign DNA, known as the SCC mec element, which is not normally found in S. aureus.…”

mentioning

confidence: 99%

Microarray Technology for the Detection of Antimicrobial Resistance Genes Present in Hospital and Community Acquired Methicillin Resistant S.aureus Strains

Licker¹,

Anghel²,

Moldovan³

et al. 2017

Rev. Chim.

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) represents a real burden for the modern medicine. One of the most frecvently isolated hospital acquired (HA) pathogens wordlwide, is Methicillin resistant Staphylococcus aureus (MRSA). Recently not only HA, but also community-acquired MRSA (CA-MRSA) infections have been reported. A prospective study was performed between February 2009 and October 2010, with the aim to investigate bacterial resistance of CA-MRSA and HA-MRSA. DNA microarray technology has been used for the detection of 4 AMR genes for the studied MRSA strains. A number of 218 HA- S.aureus strains have been isolated, from which 89 (40. 82%) were MRSA. In the community, 1.553 S.aureus strains were isolated, out of which, 356 (22. 92%) were MRSA. From these, a number of 17 HA and 12 CA �MRSA strains have been analyzed by DNA microarray technology. From 100% phenotypically described HA- MRSA, we identified mecA gene in 10 strains (58. 83%). Other 6 strains (35. 29%) have been erm(A) positive and 4 (23. 53%) - tet(O) positive. 83. 33% (10 strains) from the CA strains had mecA gene, only one (8. 33%) was erm(A) positive and 4 (33. 33%) were erm(C) positive. DNA microarray is a method allowing the concomitant scan of multiple genes and can be done within a few hours. That type of rapid and reliable methods for antimicrobial sensitivity tests are important to start an appropriate therapy.

show abstract

“…It may be argued that Msp I and Bln I restriction enzyme fragments can be used in the identification of medically important Candida spp. Further studies are needed to develop this kind of restriction profile to be used in the identification of candidal strains [237]. Restriction enzyme analysis of C. albicans and non- Candida albicans Candida spp.…”

Section: Molecular Methods Used To Detect Yeastsmentioning

confidence: 99%

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

et al. 2019

View full text Add to dashboard Cite

Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.

show abstract

Identification of Medically Important Candida Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of the rDNA ITS1 and ITS2 Regions

Cited by 7 publications

References 25 publications

High prevalence of asymptomatic nosocomial candiduria due to Candida glabrata among hospitalized patients with heart failure: a matter of some concern?

High prevalence of asymptomatic nosocomial candiduria due to Candida glabrata among hospitalized patients with heart failure: a matter of some concern?

Microarray Technology for the Detection of Antimicrobial Resistance Genes Present in Hospital and Community Acquired Methicillin Resistant S.aureus Strains

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

Contact Info

Product

Resources

About