Identification of medicinal plants within the Apocynaceae family using ITS2 and psbA-trnH barcodes

Lv, Yana; Yang, Chun-Yong; Shi, Linchun; Zhang, Zhonglian; Xu, An-Shun; Zhang, Lixia; Li, Xuelan; Li, Haitao

doi:10.1016/s1875-5364(20)30071-6

Cited by 23 publications

(15 citation statements)

References 62 publications

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“…Therefore, ITS2 was identified as a good barcode for the purposes of identification and authentication of herbal medicines used as raw materials, processed materials, and finished products [ 39 , 40 , 41 , 42 ]. Additionally, many research articles have reported the need to integrate trnH-psbA and ITS2 regions for plant species identification [ 43 , 44 , 45 , 46 ]. In order to establish more convenient and rapid identification processes, the ITS2 secondary structures of these three species were predicted.…”

Section: Discussionmentioning

confidence: 99%

“…It has been stated that the secondary structure of ITS2 can be useful for species identification at the molecular morphological characteristic level. This structure can be applied to identify various herbal medicines such as Ligusticum sinense (Apiaceae) [ 47 ]; Rhodiola crenulata and R. rosa (Crassulacea e ) [ 48 ]; Trachelospermum jasminoides (Apocynaceae) [ 49 ], the Chinese medicinal plant in Apocynaceae [ 46 ]; and various other ingredients of the traditional Chinese herbal remedy “Mu Tong” [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

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Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than

Thariwong

Intharuksa

Sirisa-ard

et al. 2021

Plants

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The Pikad Tri-phol-sa-mut-than (TS) remedy, a Thai traditional medicine, is officially recorded in Tamra Paetsart Sonkrau Chabub Anurak for its capabilities in treating kidney deficiency. TS remedy is composed of three fruit species—Aegle marmelos (L.) Corrêa., Coriandrum sativum L., and Morinda citrifolia L.—in an equal part by weight. The quality of the raw material is one of the essential factors that can affect the effectiveness and safety of treatment by herbal remedy. The pharmacognostic evaluation and DNA barcode of the three fruit species and TS remedy were performed in this study to authenticate them from contamination, and to provide the scientific database for further uses. Macroscopic and microscopic examination, chemical profile by TLC, and DNA barcoding were employed to positively identify the raw materials bought from the herbal market, especially the powder form. Consequently, the outcomes of this investigation can be used to develop an essential and effective tool for the authentication of crude drugs and herbal remedies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than

Thariwong

Intharuksa

Sirisa-ard

et al. 2021

Plants

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“…The success rate of amplification and sequencing of psbA-trnH and trnC-petN fragment was 100%, and the identification rate of two marker combinations was 87.5%. The PCR amplification and sequencing success rates for psbA-trnH in 122 plant samples of Apocynaceae were 100% and 61%, and the identification efficiency at the species level is 82% [ 31 ]. A study used trnC-petN and other markers to construct the relationships and biogeographic diversification history of Cissus [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and phylogenetic analysis of the genus Syringa based on chloroplast genomic DNA barcoding

Yao

Runfang²,

Liu³

et al. 2022

PLoS ONE

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DNA barcoding is a supplementary tool in plant systematics that is extensively used to resolve species-level controversies. This study assesses the significance of using two DNA barcoding loci (e.g., psbA-trnH and trnC-petN) in distinguishing 33 plant samples of the genus Syringa. Results showed that the average genetic distance K2P of psbA-trnH DNA marker was 0.0521, which is much higher than that of trnC-petN, which is 0.0171. A neighbor-joining phylogenetic tree based on psbA-trnH and trnC-petN indicated that the identification rate of psbA-trnH and trnC-petN alone were 75% and 62.5%, respectively. The barcode combination of psbA-trnH+trnC-petN could identify 33 samples of the genus Syringa accurately and effectively with an identification rate of 87.5%. The 33 Syringa samples were divided into four groups: Group I is series Syringa represented by Syringa oblata; Group II is series Villosae represented by Syringa villosa; Group III is series Pubescentes represented by Syringa meyeri; and Group IV is section Ligustrina represented by Syringa reticulata subsp. pekinensis. These research results provided strong evidence that the combinatorial barcode of psbA-trnH+trnC-petN had high-efficiency identification ability and application prospects in species of the genus Syringa.

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“…The barcodes ITS and psbA-trnH showed the highest number of polymorphic sites and nucleotide diversity with values of 27/0.0543 and 26/0.0336, respectively (Table 2). Studies conducted with ITS and psbA-trnH show that both loci are powerful for differentiating Apocynaceae species [64]. The psbA-trnH region has been used as a DNA barcode to authenticate several plants with similar morphological characteristics [58,65].…”

Section: Dna Barcode Analysismentioning

confidence: 99%

Essential Oil Composition and DNA Barcode and Identification of Aniba species (Lauraceae) Growing in the Amazon Region

et al. 2021

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Lauraceae species are widely represented in the Amazon, presenting a significant essential oil yield, large chemical variability, various biological applications, and high economic potential. Its taxonomic classification is difficult due to the accentuated morphological uniformity, even among taxa from a different genus. For this reason, the present work aimed to find chemical and molecular markers to discriminate Aniba species collected in the Pará State (Brazil). The chemical composition of the essential oils from Aniba canelilla, A. parviflora, A. rosaeodora, and A. terminalis were grouped by multivariate statistical analysis. The major compounds were rich in benzenoids and terpenoids such as 1-nitro-2-phenylethane (88.34–70.85%), linalool (15.2–75.3%), α-phellandrene (36.0–51.8%), and β-phellandrene (11.6–25.6%). DNA barcodes were developed using the internal transcribed spacer (ITS) nuclear region, and the matK, psbA-trnH, rbcL, and ycf1 plastid regions. The markers psbA-trnH and ITS showed the best discrimination for the species, and the phylogenic analysis in the three- (rbcL + matK + trnH − psbA and rbcL + matK + ITS) and four-locus (rbcL + matK + trnH − psbA + ITS) combination formed clades with groups strongly supported by the Bayesian inference (BI) (PP:1.00) and maximum likelihood (ML) (BS ≥ 97%). Therefore, based on statistical multivariate and phylogenetic analysis, the results showed a significant correlation between volatile chemical classes and genetic characteristics of Aniba species.

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Identification of medicinal plants within the Apocynaceae family using ITS2 and psbA-trnH barcodes

Cited by 23 publications

References 62 publications

Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than

Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than

Identification and phylogenetic analysis of the genus Syringa based on chloroplast genomic DNA barcoding

Essential Oil Composition and DNA Barcode and Identification of Aniba species (Lauraceae) Growing in the Amazon Region

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