Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides

Sun, Genlou; Fahima, Tzion; Korol, Abraham B.; Turpeinen, Timo; Grama, A.; Ronin, Yefim; Nevo, Eviatar

doi:10.1007/s001220050604

Cited by 114 publications

(45 citation statements)

References 22 publications

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“…However, only the two T. dicoccoides-derived stripe rust resistance genes Yr15 and YrH52 have been tagged using molecular markers (Sun et al, 1997 ;Langridge & Chalmers, 1998 ;Peng et al, 1999). Yr15 has been associated with six markers, including four RAPDs, one RFLP and one microsatellite (Sun et al, 1997 ;Chague! et al, 1999).…”

Section: Wheat Stripe Rust Resistance Locimentioning

confidence: 99%

“…Results from QTL studies cannot be reimplemented through marker-assisted selection because of uncertainty about whether the QTLs identified are real, and whether the identified QTLs segregate in the breeding population (Spelman & Bovenhuis, 1998b). Marker-assisted selection of economically important traits conferred by single major genes has also been mentioned or generally discussed in many publications (Talbert, 1993 ;Williams et al, 1994 ;Feuillet et al, 1995 ;Hartl et al, 1995 ;McCouch et al, 1997 ;Sun et al, 1997 ;Hausner et al, 1999) and even conducted in tomato for the Tm-2 locus (Young & Tanksley, 1989) and for the nematode resistance gene Gro1 (Ballvora et al, 1995). Markers associated with stress responses have practical application in accelerating breeding programmes (marker-assisted selection) aimed at improving the barley crop by introgressing genes from selected wild genotypes (Forster et al, 1997).…”

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confidence: 99%

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Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F₂ generation in wild emmer wheat

et al. 2000

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A new stripe rust resistance gene, YrH52, derived from the unique Mount Hermon population of wild emmer wheat (Triticum dicoccoides) in Israel, was previously located on chromosome 1B. The main objectives of the present study were to increase marker density in the vicinity of the YrH52 gene using additional microsatellite markers, and to evaluate the accuracy and efficiency of marker-assisted selection on this gene in the F # generation. By means of 70 additional microsatellite primer pairs, 150 individuals of the F # mapping population were genotyped. Among 202 marker loci, 20 were found to be linked to the YrH52 gene with log-likelihood (LOD) scores ranging from 3.84 to 58.82, and linkage distances ranging from 0.33 to 41.39 cM. A genetic map was constructed of chromosome 1B, consisting of 23 markers and the YrH52 gene, with a total map length of 149.5 cM. Most of the markers were located in the region close to YrH52, which was flanked by Xgwm413 and Xgwm273a with map distances of 1.3 and 2.7 cM, respectively. The accuracy and efficiency of marker-assisted selection were calculated as AMAS and EMAS, respectively, for homozygous resistant genotypes of YrH52 gene in the F # generation. AMAS and EMAS for homozygous resistant genotypes of the YrH52 gene in the F # generation showed linear and significant negative correlation with the map distance between marker and target gene or between the two bracketing markers. It was concluded that the YrH52 region on chromosome 1B is significantly more enriched by microsatellite markers than the previously published map ; that a single microsatellite marker is efficient for marker-assisted selection of homozygous resistant genotypes of YrH52 gene in the F # generation when the map distance is 5.0 cM ; and that when two markers are used AMAS can be dramatically improved and becomes relatively stable, whereas EMAS will not be obviously improved and will still vary linearly with map distance.

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Section: Wheat Stripe Rust Resistance Locimentioning

confidence: 99%

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confidence: 99%

Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F₂ generation in wild emmer wheat

et al. 2000

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“…The utilization of these genes has minimized the application of fungicides, contributing to reduce both environmental contamination and production costs. Therefore, it is not surprising that rust resistance genes were frequent targets in restriction fragment length polymorphism (RFLP) mapping studies in wheat (Schachermayr et al 1994(Schachermayr et al , 1995Autrique et al 1995;Dedryver et al 1996;Nelson et al 1997;Sun et al 1997;Dubcovsky et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Development of PCR markers for the wheat leaf rust resistance gene Lr47

Helguera¹,

Khan²,

Dubcovsky³

2000

Theor Appl Genet

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The leaf rust resistance gene Lr47 confers resistance to a wide spectrum of leaf rust strains. This gene was recently transferred from chromosome 7 S of Triticum speltoides to chromosome 7 A of hexaploid wheat Triticum aestivum. To facilitate the transfer of Lr47 to commercial varieties, the completely linked restriction fragment length polymorphism (RFLP) locus Xabc465 was converted into a PCR-based marker. Barley clone ABC465 is orthologous to the type-I wheat sucrose synthase gene and primers were designed for the conserved regions between the two sequences. These conserved primers were used to amplify, clone and sequence different alleles from T. speltoides and T. aestivum. This sequence information was then used to identify the T. speltoides sequence, detect allele-specific mutations, and design specific primers. Cosegregation of the PCR product of these primers and the T. speltoides chromosome segment was confirmed in four backcross-populations. To complement this dominant marker, a cleavage amplified polymorphic sequence (CAPS) was developed for the 7 A allele of Xabc465. This CAPS marker is useful to select homozygous Lr47 plants from F 2 or backcross-F 2 segregating populations, and in combination with the T. speltoides-specific primers is expected to facilitate the deployment of Lr47 in new bread wheat varieties.

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“…For common bunt, also known as stinking smut and covered smut, the polymorphic RAPD marker, BW553, was identiied between resistant and susceptible NILs [58]. Furthermore, identiication of RAPD markers linked to the Yr15 gene controlling stripe rust resistance was conducted using 340 RAPD primers, six of which were detected to be polymorphic [59]. The OPB13 RAPD marker was the only one that produced polymorphism in 123 F 2 individuals and showed that it was linked to Yr15 through screening a series of NILs each consisted of a diferent gene for Hessian ly resistance using 1600 random 10-mer primers.…”

Section: Random Ampliied Polymorphic Dna (Rapd) Markersmentioning

confidence: 99%

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Kim¹,

Kim²,

Jang³

2017

Wheat Improvement, Management and Utilization

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This chapter addresses the development and use of molecular markers for yield enhancement in wheat. Since their key goal for breeding is to maximize yield, extensive eforts have been made toward the improvement of yield. Agronomic traits related to yield, yield-related, disease resistance, and abiotic stresses are considered to be quantitative traits (QTLs), also known as complex traits, because they are controlled by numerous genes and are afected by environmental factors. Researchers have been studying such traits in the past decades for the development of molecular markers which can be used in various wheat breeding studies mainly involving restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), single nucleotide polymorphism (SNP), random ampliied polymorphic DNA (RAPD), and ampliied fragment length polymorphism (AFLP). Furthermore, the advent of next-generation sequencing (NGS) has accelerated the discovery of agronomically important genes. All of the technologies have enabled great advances for increasing the productivity of wheat. Here, the past history of irst-generation sequencing, present status of second-generation sequencing, and future potential of translational genomics linked to the yield will be discussed.

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Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides

Cited by 114 publications

References 22 publications

Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F₂ generation in wild emmer wheat

Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F₂ generation in wild emmer wheat

Development of PCR markers for the wheat leaf rust resistance gene Lr47

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

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Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides

Cited by 114 publications

References 22 publications

Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F2 generation in wild emmer wheat

Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F2 generation in wild emmer wheat

Development of PCR markers for the wheat leaf rust resistance gene Lr47

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

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Product

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Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F₂ generation in wild emmer wheat

Microsatellite high‐density mapping of the stripe rust resistance gene YrH52 region on chromosome 1B and evaluation of its marker‐assisted selection in the F₂ generation in wild emmer wheat