Identification of monocot chimeric jacalin family reveals functional diversity in wheat

Ma, Qing‐Hu; Han, Jiaqi

doi:10.1007/s00425-020-03548-2

Cited by 10 publications

(15 citation statements)

References 40 publications

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“…For example, BGAF from Sorghum bicolor is highly selective towards GalNac [ 35 ], whereas maize BGAF (H95 Inbred Line) shows a recognition pattern similar to OsJAC1, thus responding to galactose and mannose [ 36 ]. Recently, Ma et al showed that homologs of OsJAC1 in wheat are able to interact with galactose and mannose [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Initial Structural Characterization of the Monocot Chimeric Jacalin OsJAC1

Huwa

Weiergräber

Kirsch

et al. 2021

IJMS

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The monocot chimeric jacalin OsJAC1 from Oryza sativa consists of a dirigent and a jacalin-related lectin domain. The corresponding gene is expressed in response to different abiotic and biotic stimuli. However, there is a lack of knowledge about the basic function of the individual domains and their contribution to the physiological role of the entire protein. In this study, we have established a heterologous expression in Escherichia coli with high yields for the full-length protein OsJAC1 as well as its individual domains. Our findings showed that the secondary structure of both domains is dominated by β-strand elements. Under reducing conditions, the native protein displayed clearly visible transition points of thermal unfolding at 59 and 85 °C, which could be attributed to the lectin and the dirigent domain, respectively. Our study identified a single carbohydrate-binding site for each domain with different specificities towards mannose and glucose (jacalin domain), and galactose moieties (dirigent domain), respectively. The recognition of different carbohydrates might explain the ability of OsJAC1 to respond to different abiotic and biotic factors. This is the first report of specific carbohydrate-binding activity of a DIR domain, shedding new light on its function in the context of this monocot chimeric jacalin.

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Section: Discussionmentioning

confidence: 99%

Biochemical and Initial Structural Characterization of the Monocot Chimeric Jacalin OsJAC1

Huwa

Weiergräber

Kirsch

et al. 2021

IJMS

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show abstract

“…In addition, the DIR-c subfamily is only found in angiosperm monocots. The DIR genes of the DIR-c subfamily always combine with the jacalin-like domain to contribute to the defense against insects [15,51,52]. P. trichocarpa has three subfamilies (DIR-a, b/d, and e).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization of Dirigent Proteins in Populus: Gene Expression Variation and Expression Pattern in Response to Marssonina brunnea and Phytohormones

Sun

Zhou

Wei

et al. 2021

Forests

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Marssonina brunnea causes a major disease that limits poplar growth. Lignin and lignan play essential roles in protecting plants from various biological stresses. Dirigent (DIR) proteins are thought to control the stereoselective coupling of coniferyl alcohol in the formation of lignan and lignin. DIR family members have been well studied in several plant species, but no previous detailed genome-wide analysis has been carried out in forest trees, such as poplar. We identified 40 PtDIR genes in Populus trichocarpa and classified them into three subgroups (DIR-a, DIR-b/d, and DIR-e) based on phylogenetic analyses. These genes are distributed on 11 poplar chromosomes, and 80% of PtDIRs (32/40) are intronless. The cis-element analysis inferred that PtDIRs possess many types of biological and abiotic stress-response cis-elements. We also analyzed intra- and inter-specific collinearity, which provided deep insights into the evolutionary characteristics of the poplar DIR genes. Analyses of the protein tertiary structure and critical amino acid residues showed that PtDIR7–10 and PtDIR13–16, which belong to the DIR-a subfamily, might be involved in the regio- and stereo-selectivity of bimolecular phenoxy radical coupling in poplars. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis revealed different expression patterns for the PtDIR genes of P. trichocarpa and the PeDIR genes of ‘Nanlin 895’ in various tissues. Additionally, we analyzed responses of PeDIRs to M. brunnea and different phytohormone treatments (abscisic acid, salicylic acid, methyl jasmonate, and ethylene) in ‘Nanlin 895’. The results showed that at least 18 genes responded strongly to M. brunnea, and these PeDIRs also showed significant responses to phytohormones. These results suggest that DIR genes are involved in the poplar defense response against M. brunnea, and this study will provide fundamental insights for future research on poplar DIR genes.

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“…Chimeric lectins containing a Kelch motif, an F-box motif, a tyrosine protein kinase domain or an NB-ARC domain have also been documented. Many of these chimeric lectins have been proposed to play a role in plant immunity and defense [12,83,[113][114][115]. Jacalin domains have also been identified as part of nucleotide-binding and leucine-rich repeat receptors in cereals and were suggested to act as decoys, trapping effectors of pathogens and triggering a defense responses [116].…”

Section: Stress-inducible Jacalin-related Lectinsmentioning

confidence: 99%

“…Cereal species contain extended families of chimeric jacalin-related lectins, encoding stress-inducible mannose-binding lectins, that play a role in plant defense and stress signaling [123] Tobacco overexpression lines for the mannose-binding jacalin-related lectins with a dirigent domain from wheat showed increased tolerance to dehydration for TaMCJ3, whereas TaMCJ1 lines revealed reduced bacterial growth for P. syringae [113,114]. Similarly the mannose-binding OsJAC1 from rice contributes to broad-spectrum resistance to several pathogens such as Magnaporthe oryzae, Rhizoctonia solani, Xanthomonas oryzae [124].…”

Section: Stress-inducible Jacalin-related Lectinsmentioning

confidence: 99%

Review: The multiple roles of plant lectins

Coninck

Damme

2021

Plant Science

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Identification of monocot chimeric jacalin family reveals functional diversity in wheat

Cited by 10 publications

References 40 publications

Biochemical and Initial Structural Characterization of the Monocot Chimeric Jacalin OsJAC1

Biochemical and Initial Structural Characterization of the Monocot Chimeric Jacalin OsJAC1

Genome-Wide Characterization of Dirigent Proteins in Populus: Gene Expression Variation and Expression Pattern in Response to Marssonina brunnea and Phytohormones

Review: The multiple roles of plant lectins

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